Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Effect of a Common Medium on the Growth of Nitrogen Fixer Rhizobium and Phosphate Solubilizer Bacillus megaterium

질소고정균(Rhizobium)과 인산가용화균(Bacillus megaterium)의 동시배양을 위한 배양조건 탐색

  • Poonguzhali, Selvaraj (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Thangaraju, Muthu (Department of Agricultural Microbiology, Tamil Nadu Agricultural University) ;
  • Ryu, Jyung-Hyun (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Chung, Keun-Yook (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • Received : 2005.01.14
  • Accepted : 2005.02.07
  • Published : 2005.02.28
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Abstract

Mass culturing of two beneficial organisms used as biofertilizers for crops would reduce the risks in production and minimize the capital involved and this demands appropriate media that supports both organism and also selection of organisms that are not antagonistic to each other. A study was initiated to culture a nitrogen fixer (Rhizobium) and phosphate solubilizer (Bacillus megaterium) in a single medium and to study their growth patterns and shelf life in carrier. The growth of Rhizobium and Bacillus megaterium was assessed in different media and a slight modification in the traditional yeast extract mannitol media promoted the growth of both the organisms. The growth of the individual organisms in the modified medium was assessed by estimating the population at regular intervals and compared to their original medium. Maximum population of Rhizobium and phosphobacteria was at 60 hr when the phosphiobacteria inoculation of later was after 48 hr of Rhizobium inoculation. The shelf life of the individual inoculants in the inoculant containing both the organism in a sterile carrier base revealed no significant differences compared to individual organisms inoculated in a sterilized carrier. The population of both organisms in carrier based mixed inoculant remained at $10^8$ cells till 90 days.

다양한 기능을 보유하고있는 농업용 유용미생물을 경제적으로 대량생산하기 위하여는 동시에 다양한 미생물이 상호간에 길항관계를 갖지않고 잘자랄수있는 배지의 선택이 필수적으로 요망된다. 본실험은 질소고정균 (Rhizobium)과 인산가용화균 (Phosphobacteria)을 yeast extract mannitol변형배지를 포함한 다양한배지에서 함께 배양시 각각의 성장을 극대화할 수 있는 접종시차를 규명하고 각 조건에서 생산된 미생물의 효능유지기간을 비교하였다. 인산가용화균과 질소고정균을 동시배양시 최대 수율을 얻기위한 조건은 인산가용화균을 먼저접종한 후 48시간후에 질소고정균을 접종한 후 60시간 배양하는것이었다. 이러한 조건으로 배양하였을 때 생산된 균의 효능유지기간은 각각의 균을 단독배양한것과 비교하여 차이가 나타나지 않았다. 또한, 이러한 조건에서 생산된 미생물의 개체수는 담체에 고정시킨후에도 60일까지 변화없이 유지되었다.

Keywords

References

  1. Azcon. C. G., D. Aguilar, and J. M. Barea. 1978. Effects of interactions between different culture fractions of phosphobacteria and Rhizobium on mycorrhizal infection. Can. J. Microbiol. 24:520-523 https://doi.org/10.1139/m78-085
  2. Balasubramanian, A. 1996. Potential of biofertilizers in organic farming. p. 58-65. In G. K Veeresh. K Shivashankar. and M. A. Singlachar (ed.) Organic farming and sustainable agriculture, Ravi Publication. Bangalore, India
  3. Baldev, R, and R G. Pareek. 2000. Etlect of phosphorous, sulphur and phosphate solubilizing bacteria on yield. oil content and nutrient uptake by mustard (Brassica juncea L.) (zem and coss). Agric. Sci. Digest. 20:241-243
  4. Baldwin. I. L.. and E. B. Fred. 1927. Fermentation characters of the root nodule bacteria of the leguminosae. Soil Sci. 24:217-230 https://doi.org/10.1097/00010694-192709000-00006
  5. Dube. J. N., S. L. Namdeo, and M. S. Johar. 1975. Coal as a carrier of rhizobia. Curr. Sci. 44:434
  6. Frank. 2001. Biofertilizers in IPNS. Fertilizer News 46: 15-24
  7. Gaind, S.. and A. C. Gaur. 1990. Role of phosphate solubilizing inoculants as influenced by type of carrier, high temperature and low moisture. Can. J. Microbiol. 36:846-849 https://doi.org/10.1139/m90-146
  8. Glenn. A. R., I. A. Mckay, R. Arwas, and M. J. Dilworth. 1984. Sugar metabolism and the symbiotic properties of carbohydrate mutants of Rhizobium leguminosarum. J. Gen. Microbiol. 130:231-237
  9. Gupta, R. S., P. Singh, and M. S. Pandher. 1998. Role of phosphorus solubilizing microorganisms in P economy and crop yield. p. 95-101. In B. D. Kaushik (ed.) Soil plant microbe interaction in relation to integrated nutrient management. IARI. New Delhi. India
  10. Joshi, N. V. 1920. Studies on the root nodule organisms of the leguminous plants, India Dept. Agrl. Mem. Bact. Sect. 1:247-276
  11. Lakshmi Priya, D. 1997. Studies on phosphate solubilizing bacteria. M.Sc. (Ag.) Thesis, Tamil Nadu Agricultural University, Coimbatore, India
  12. Lopreto, C. R., L. A. Mazza, and A. P. Balatti. 1972. Infiuencia ce los components del media de cultivo subre el tiempo de generacion de una cepa de R. japonicum. An. Soc. Cient Argent. p. 35-47
  13. Meyer. J. R., and R G. Lindennan. 1986. Response of subterranean clover to dual inoculation with vesicular arbuscular mycorrhizal fungi and a plant growth promoting bacterium, Pseudomonas putida. Soil Biol. Biochem. 18: 185-190 https://doi.org/10.1016/0038-0717(86)90025-8
  14. Pal, M. 1998. Microorganisms as fertilizer and plant nutrition. p. 91-94. In B. D. Kaushik (ed.) Soil plant microbe interaction in relation to integrated nutrient management. JARI. New Delhi, India
  15. Ramesh. N., and R. N. Sabale. 2001. Effect of phosphate fertilization phosphate solubilizer and plant production on yield and quality of summer groundnut. Indian J. Agron. 46: 156-161
  16. Romanov, V. I.. and E. Martinez Romero. 1994. Sucrose transport and hydrolysis in R. tropicl. Plant Soil 161 :91-96 https://doi.org/10.1007/BF02183088
  17. Saxena, A. K, and K. V. B. R. Tilak. 1994. Interaction among beneficial soil microorganisms. Indian J. Microbiol. 34:91-106
  18. Senthilkumar. M. 1999. Improved methodologies for legume inoculant production. M.S. (Ag.) Thesis, Tamil Nadu Agricultural University. Coimbatore, India
  19. Smith, R. S. 1992. Legume inoculant formulation and application. Can. J. Microbiol. 38:485-492 https://doi.org/10.1139/m92-080
  20. Stevenson, F. J. 1986. Cycles of soil carbon, nitrogen, phosphorous, sulfur micronutrients. Wiley, New York, NY, USA
  21. Tippannavar, C. M., S. Janagirdar, and V. S. Vemmimath. 2001, Influence of Rhizobium inoculants on pigeon pea in dry zone of Kamataka. Agric. Sci. Digest 21:167-169
  22. Tiwari, V. N., A. N. Pathak, and K. K. Lehri. 1988. Manurial value of compost enriched with rock phosphate and microbial inoculants to green gram. J. Ind. Soc. Soil. Sci. 36:280-283