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Effect of a Common Medium on the Growth of Nitrogen Fixer Rhizobium and Phosphate Solubilizer Bacillus megaterium  

Poonguzhali, Selvaraj (Department of Agricultural Chemistry, Chungbuk National University)
Thangaraju, Muthu (Department of Agricultural Microbiology, Tamil Nadu Agricultural University)
Ryu, Jyung-Hyun (Department of Agricultural Chemistry, Chungbuk National University)
Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University)
Chung, Keun-Yook (Department of Agricultural Chemistry, Chungbuk National University)
Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.38, no.1, 2005 , pp. 8-14 More about this Journal
Abstract
Mass culturing of two beneficial organisms used as biofertilizers for crops would reduce the risks in production and minimize the capital involved and this demands appropriate media that supports both organism and also selection of organisms that are not antagonistic to each other. A study was initiated to culture a nitrogen fixer (Rhizobium) and phosphate solubilizer (Bacillus megaterium) in a single medium and to study their growth patterns and shelf life in carrier. The growth of Rhizobium and Bacillus megaterium was assessed in different media and a slight modification in the traditional yeast extract mannitol media promoted the growth of both the organisms. The growth of the individual organisms in the modified medium was assessed by estimating the population at regular intervals and compared to their original medium. Maximum population of Rhizobium and phosphobacteria was at 60 hr when the phosphiobacteria inoculation of later was after 48 hr of Rhizobium inoculation. The shelf life of the individual inoculants in the inoculant containing both the organism in a sterile carrier base revealed no significant differences compared to individual organisms inoculated in a sterilized carrier. The population of both organisms in carrier based mixed inoculant remained at $10^8$ cells till 90 days.
Keywords
Rhizobium sp.; Bacillus megaterium; Dual inoculation; N-fixer; Phosphate solubilizer;
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1 Azcon. C. G., D. Aguilar, and J. M. Barea. 1978. Effects of interactions between different culture fractions of phosphobacteria and Rhizobium on mycorrhizal infection. Can. J. Microbiol. 24:520-523   DOI   ScienceOn
2 Baldev, R, and R G. Pareek. 2000. Etlect of phosphorous, sulphur and phosphate solubilizing bacteria on yield. oil content and nutrient uptake by mustard (Brassica juncea L.) (zem and coss). Agric. Sci. Digest. 20:241-243
3 Glenn. A. R., I. A. Mckay, R. Arwas, and M. J. Dilworth. 1984. Sugar metabolism and the symbiotic properties of carbohydrate mutants of Rhizobium leguminosarum. J. Gen. Microbiol. 130:231-237
4 Gupta, R. S., P. Singh, and M. S. Pandher. 1998. Role of phosphorus solubilizing microorganisms in P economy and crop yield. p. 95-101. In B. D. Kaushik (ed.) Soil plant microbe interaction in relation to integrated nutrient management. IARI. New Delhi. India
5 Joshi, N. V. 1920. Studies on the root nodule organisms of the leguminous plants, India Dept. Agrl. Mem. Bact. Sect. 1:247-276
6 Meyer. J. R., and R G. Lindennan. 1986. Response of subterranean clover to dual inoculation with vesicular arbuscular mycorrhizal fungi and a plant growth promoting bacterium, Pseudomonas putida. Soil Biol. Biochem. 18: 185-190   DOI   ScienceOn
7 Saxena, A. K, and K. V. B. R. Tilak. 1994. Interaction among beneficial soil microorganisms. Indian J. Microbiol. 34:91-106
8 Balasubramanian, A. 1996. Potential of biofertilizers in organic farming. p. 58-65. In G. K Veeresh. K Shivashankar. and M. A. Singlachar (ed.) Organic farming and sustainable agriculture, Ravi Publication. Bangalore, India
9 Pal, M. 1998. Microorganisms as fertilizer and plant nutrition. p. 91-94. In B. D. Kaushik (ed.) Soil plant microbe interaction in relation to integrated nutrient management. JARI. New Delhi, India
10 Romanov, V. I.. and E. Martinez Romero. 1994. Sucrose transport and hydrolysis in R. tropicl. Plant Soil 161 :91-96   DOI   ScienceOn
11 Tiwari, V. N., A. N. Pathak, and K. K. Lehri. 1988. Manurial value of compost enriched with rock phosphate and microbial inoculants to green gram. J. Ind. Soc. Soil. Sci. 36:280-283
12 Frank. 2001. Biofertilizers in IPNS. Fertilizer News 46: 15-24
13 Lopreto, C. R., L. A. Mazza, and A. P. Balatti. 1972. Infiuencia ce los components del media de cultivo subre el tiempo de generacion de una cepa de R. japonicum. An. Soc. Cient Argent. p. 35-47
14 Smith, R. S. 1992. Legume inoculant formulation and application. Can. J. Microbiol. 38:485-492   DOI
15 Gaind, S.. and A. C. Gaur. 1990. Role of phosphate solubilizing inoculants as influenced by type of carrier, high temperature and low moisture. Can. J. Microbiol. 36:846-849   DOI
16 Senthilkumar. M. 1999. Improved methodologies for legume inoculant production. M.S. (Ag.) Thesis, Tamil Nadu Agricultural University. Coimbatore, India
17 Ramesh. N., and R. N. Sabale. 2001. Effect of phosphate fertilization phosphate solubilizer and plant production on yield and quality of summer groundnut. Indian J. Agron. 46: 156-161
18 Lakshmi Priya, D. 1997. Studies on phosphate solubilizing bacteria. M.Sc. (Ag.) Thesis, Tamil Nadu Agricultural University, Coimbatore, India
19 Tippannavar, C. M., S. Janagirdar, and V. S. Vemmimath. 2001, Influence of Rhizobium inoculants on pigeon pea in dry zone of Kamataka. Agric. Sci. Digest 21:167-169
20 Baldwin. I. L.. and E. B. Fred. 1927. Fermentation characters of the root nodule bacteria of the leguminosae. Soil Sci. 24:217-230   DOI
21 Stevenson, F. J. 1986. Cycles of soil carbon, nitrogen, phosphorous, sulfur micronutrients. Wiley, New York, NY, USA
22 Dube. J. N., S. L. Namdeo, and M. S. Johar. 1975. Coal as a carrier of rhizobia. Curr. Sci. 44:434