Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Genomic Relationship Among 25 Species of Mammillaria Haw. as Revealed by Isozyme and Protein Polymorphism

  • Published : 2005.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Buffer soluble protein and five isozymes were analyzed to assess the inter specific relationship among 25 species of the genus Mammillaria Haw. A total of 102 types of proteins were resolved, out of which eighty-six types were found to be polymorphic and only two were unique. A total of 248 bands (isoforms) were detected for 5 isozymes, among them only 4 were found to be monomorphic and 35 were exclusive. Mantel 'Z' statistics revealed wide variations in the correlation among different enzymes. The correlation value 'r' was the highest in case of esterase with pooled data of all the five enzymes. The dendrogram constructed on the basis of pooled data (protein and allozyme) divided the species into two major clusters containing 14 and 11 members respectively. The species M. matudae and M. bella were found to be the most closely related while M. decipience and M. camptroticha were distantly apart. The present study gave an indication of usefulness of the isozyme and protein markers for genetic discrimination between different species of Mammillaria.

Keywords

References

  1. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  2. Cardy BJ, Stuber CW, Goodman MM (1981) Techniques for starch gel electophoresis of enzymes from maize (lea mays L.). Institute of Statistics micrograph, series no. 1317, North Carolina State University, Raleigh, North Carolina
  3. Chang CS, Kim H, Park, TY (2003). Patterns of allozyme diversity in several selected rare species in Korea and implication for conservation. Biodiversity and Conservation 12: 529-544 https://doi.org/10.1023/A:1022424918477
  4. Choer E, Augustin E, Antunes IF, Dasilva JB, Peters JA (1999) Cultivar identification and genetic purity analysis of beans (Phaseolus vulgaris L.) through isoenzymatic patterns. Seed Science and Technology 27: 517-523
  5. Chrambach A, An Der Lan B, Mohrmann H, Felgenhauer K (1967) Electrophoresis 2: 279 https://doi.org/10.1002/elps.1150020505
  6. Dadlani M, Varier A (1993) Electrophoresis for variety identification. Technical Bulletin, Division of Seed Science Technology, I. A. R.I., New Delhi, India
  7. Desborough SL, Peloquin SJ (1966) Disc electrophoresis of tuber proteins from solanum species in interspecific hybrids. Phytochemistry 5: 727-733 https://doi.org/10.1016/S0031-9422(00)83653-5
  8. Desborough SL, Peloquin SJ(1968) Potato variety identification by use of electrophoretic patterns of tuber proteins and enzymes. Am Potato Journal 45: 220-229 https://doi.org/10.1007/BF02849396
  9. Desborough SL, Peloquin SJ (1971) Acid phsphatase isozymes in haploids and selfs of Group Tubersum potato cultivars. Phytochemistry 10: 571-572 https://doi.org/10.1016/S0031-9422(00)94699-5
  10. Eeswara JP, Peiris BCN (2001) Isoenzymes as markers for identification of Mungbean (Vigna radiata (L.) Wilcezk). Seed Science and Technology 29: 249-254
  11. Fang G, Hammer S, Groumet R (1992) A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biofeedback 13: 52-54
  12. Fullington JC, Cole EW, Kasarda DD (1983) Quantitative sodium dodecyl sulfate polyacrylamide gel electrophoresis of total proteins: Effects of protein content. Cereal Chemistry 60: 65-71
  13. Gao L.-z., Hong S. G. De-y. (2000) Allozyme variation and population genetic structure of common wildrice Oryza rufipogon Griff. in China. Theor Appl Genet 101 :494-502 https://doi.org/10.1007/s001220051508
  14. Geburek TH, Scholz F, Knabe K, Vornweg A (1987) Genetic studies by isozyme gene loci on tolerance and sensitivity in an air polluted Pinus sylvestris field trial. Silvae Genetica 36: 49-53
  15. Gray JR, Fairbrothers DE, Quinn JA (1973) Biochemical and anatomical population variation in the Danthonia sericea complex. Botanical Gazette 134: 166-173 https://doi.org/10.1086/336699
  16. Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds), Plant Population Genetics, Breeding and Genetic Resources, Sinauer, Sunderland, MA, pp 43-63
  17. Hamrick JL, Godt MJW (1996) Effects of life-history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society of London, series B, 351: 1291-1298 https://doi.org/10.1098/rstb.1996.0112
  18. Hartl DL, Clark AG (1989) Principle of population genetics, 2nd ed. Sinauer associates, Sunderland, MA
  19. Henry RJ (2001). Plant Genotyping : The DNA Fingerprinting of Plants, CAB I Publishing, Wallingford, UK, pp 325
  20. Hunt D (1987) A new review of Mammillaria names. British Cactus and Succulent Society, Oxford, pp 105-128
  21. Hunter RL, Markert CL (1957) Histochemica demonstration of enzyme separated in zone electrophoresis in starch gels. Science 125: 1294-1295
  22. Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bulletin de la Societe Vaudense des Scinses Naturelles 44: 223-270
  23. Kumar J., Agrawal R. L., Kumar A., Garg G. K. (2001) Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis for determination of genetic purity of sunflower hybrids. Seed Science and Technology 29: 647-652
  24. Lamb MB (1991) Letts guide to cacti of the world. Charles Letts and Co., London, pp 215
  25. Lee DW, Fairbrothers DE (1973) Enzyme differentiation between adjacent hybrid and parent populations of Typha. Bulletin Torrey Botanical Club 10: 3-11
  26. Machado MDPS, Mangolin CA, Collet SAD (2000) Somatic crossing-over can induce allozyme variation in somaclones of Cereus peruvianus Mill. (Cactaceae). Haseltonia 7: 77-80
  27. Mangolin CA, Prioli AJ, Machado MFPS (1997) Allozyme variability in plants regenerated from calli of Cereus. Biochemical Genetics 35: 189-204 https://doi.org/10.1023/A:1021906226291
  28. Mantel NA (1967) The detection of disease clustering and a generalised regression approach. Cancer Research 27: 209-220
  29. Marsden C (1957) Mammillaria. Cleaver-Hume Press, London, pp 407
  30. MartinezPalacios A, Eguiarte LE, Fumier GR (1999) Genetic diversity of the endangered endemic Agave victariaereginae (Agavaceae) in the Chihuahuan Desert. American Journal of Botany 86: 1093-1098 https://doi.org/10.2307/2656971
  31. Nassar JM, Hamrick JL, Fleming TH (2001) Genetic variation and population structure of the mixed-mating cactus, Melacactus curvispinus (Cactaceae). Heredity 87: 69-79 https://doi.org/10.1046/j.1365-2540.2001.00910.x
  32. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Nat Acad Sci USA 70: 3321-3323 https://doi.org/10.1073/pnas.70.12.3321
  33. Oleary MC, Boyle TH (1998) Inheritance and linkage relationship of Isozymes in easter cactus. Journal of the American society for Horticultural Science 123: 98-103
  34. Oleary MC, Boyle TH (1999) Cultivar identification and genetic diversity within a Hatiora (Cactaceae) clonal germplasm collection using Isozymes. Journal of the American Society for Horticultural Science 124: 373-376
  35. Oleary MC, Boyle TH (2000) Diversity and distribution of Isozymes in a Schlumbergera (Cactaceae) clonal germplasm collection. Journal of the American Society for Horticultural Science 125: 81-85
  36. Panda PC, Das P (1995) Genetic resources of cacti and other succcuints. In: Chadha KL, Bhattacharjee SK (eds), Advances in Horticulture Vol. 12-Ornamental Plants, Malhotra Publishing House, New Delhi, India, pp 243-266
  37. Payne RC, Fairbrothers DE (1976) Disc electrophoresis evidence for heterozygosity and phenotypic plasticity in selected lines of Caftea arabica L. Botanical Gazette 137: 1-6 https://doi.org/10.1086/336833
  38. Pilbeam J (1987) Cacti for the connoisseur- A guide for growers and collectors. BT Batsford, London, pp 167
  39. Rohlf, FJ. 1997. NTSYS-PC: numerical taxonomy and multivariate analysis system. Exeter software, New York Rowley J (1978) The illustrated Encyclopedia of succulents. Leisure Books, pp 256
  40. Rowley J (1978) The Illustrated Encyclopedia of succulents. Leisure Books pp 256
  41. Rychter A, Lewak ST (1969) Polyacrylamide gel electrophoresis of apple seed enzymes. Acta Biochemica Polonica 4: 333-338
  42. Scandalios JG (1969) Genetic control of multiple molecular forms of enzymes in plants: a review. Biochemical Genetics 3:37-79 https://doi.org/10.1007/BF00485973
  43. Schuster D (1990) The World of Cacti. Facts on File Ltd., Oxford, UK, pp 240
  44. Scogin R (1969) Allozyme polymorphism in natural population of the genus Baptista (Leguminosae). Phytochemistry 8: 1733-1737 https://doi.org/10.1016/S0031-9422(00)85961-0
  45. Scott KO, Playford J (1996) DNA extraction technique for PCR in rain forest plant species. Biotechniques 20: 974-978
  46. Sengupta S, Chattopadhyay NC (2000) Rice varietal identification by SOS-PAGE. Seed Science and Technology 28: 871-873
  47. Siaba R (1992) The illustrated guide to cacti. Chancellor Press, London, pp 224
  48. Stegemann H, Loeschke V (1977) Oas Europaische Kartoffelsortimentund seinelndexierung. Potato Research 20: 101-110 https://doi.org/10.1007/BF02360269
  49. Turner BL (1969) Chemosystematics: recent developments. Taxon 18: 134-151 https://doi.org/10.2307/1218672
  50. Weniger D (1969) Cacti of the SouthWest. University of Texas Press, London, pp 249
  51. Wright S (1951) The genetic structure of populations. Annals of Eugenics 15: 313-354
  52. Yaakor S, De Wet JMJ (1975) Comparative electrophoresis and allozyme analysis of seed proteins from cultivated races of Sorghum. American Journal of Botany 62: 254-266 https://doi.org/10.2307/2441859