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Genomic Relationship Among 25 Species of Mammillaria Haw. as Revealed by Isozyme and Protein Polymorphism  

Mattagajasingh Ilwola (DNA Fingerprinting Laboratory, Regional Plant Resource Centre)
Acharya Laxmikanta (DNA Fingerprinting Laboratory, Regional Plant Resource Centre)
Mukherjee Arup Kumar (DNA Fingerprinting Laboratory, Regional Plant Resource Centre)
Das Premananda (DNA Fingerprinting Laboratory, Regional Plant Resource Centre)
Publication Information
Journal of Plant Biotechnology / v.7, no.2, 2005 , pp. 105-112 More about this Journal
Abstract
Buffer soluble protein and five isozymes were analyzed to assess the inter specific relationship among 25 species of the genus Mammillaria Haw. A total of 102 types of proteins were resolved, out of which eighty-six types were found to be polymorphic and only two were unique. A total of 248 bands (isoforms) were detected for 5 isozymes, among them only 4 were found to be monomorphic and 35 were exclusive. Mantel 'Z' statistics revealed wide variations in the correlation among different enzymes. The correlation value 'r' was the highest in case of esterase with pooled data of all the five enzymes. The dendrogram constructed on the basis of pooled data (protein and allozyme) divided the species into two major clusters containing 14 and 11 members respectively. The species M. matudae and M. bella were found to be the most closely related while M. decipience and M. camptroticha were distantly apart. The present study gave an indication of usefulness of the isozyme and protein markers for genetic discrimination between different species of Mammillaria.
Keywords
Isozyme; Cactus; Mammillaria; Protein; Genomic relations; Chemical characterization;
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