Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Protective Effect of the Stem Bark of Syringa velutina on Bisphenol-A in the Human Breast Cancer Cell Line and Immature Rat

사람의 유방암 세포주와 미성숙 랫드에서 정향피의 비스페놀 A 독성방어 효과

  • Jo Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Yang Se-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Cho Sung-Dae (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park Joon-Suk (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Hwang Jae-Woong (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Lee Seong-Hun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park Jung-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Lee Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kang Kyung-Sun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
  • 조은혜 (서울대학교 수의과대학 공중보건학교실) ;
  • 양세란 (서울대학교 수의과대학 공중보건학교실) ;
  • 조성대 (서울대학교 수의과대학 공중보건학교실) ;
  • 정지원 (서울대학교 수의과대학 공중보건학교실) ;
  • 박준석 (서울대학교 수의과대학 공중보건학교실) ;
  • 황재웅 (서울대학교 수의과대학 공중보건학교실) ;
  • 이성훈 (서울대학교 수의과대학 공중보건학교실) ;
  • 박정란 (서울대학교 수의과대학 공중보건학교실) ;
  • 이영순 (서울대학교 수의과대학 공중보건학교실) ;
  • 강경선 (서울대학교 수의과대학 공중보건학교실)
  • Published : 2005.03.01
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Abstract

The inhibitory activity against bisphenol-A (BPA), one of well-known endocrine disrupters was examined with the water extracts prepared from the Stem Bark of Syringa velutina (SBS). In this study, we have investigated the effect of SBS on the toxicity caused by BPA in human breast cancer cell line, MCF-7 cells and immature Sprague-Dawley rats. In the estrogen receptor-mediated proliferation assay using MCF-7 cells, BPA (16 ng/ml) induced the cell proliferation, but the water extract of SBS inhibited BPA-induced cell proliferation in a dose-dependent manner. These results are associated with PARP degradation and specific cleavage of anti-apoptotic protein Bcl-2 of apoptotic regulatory factors. Additionally, the BPA (400 mg/100 g) significantly induced the increase of the uterine and virginal weights, while SBS (50 mg/100 g) showed the inhibitory action against BPA, i.e. caused the increase of estrogen-related organ weights in immature rat uterotrophic assay. Taken together, the present data suggest that SBS may have anti-toxicity activities against BPA in vitro and in vivo systems. SBS may be capable of inhibiting adverse effects of BPA such as reproductive disorder.

Keywords

References

  1. Burlacu, A. (2003): Regulation of apoptosis by BcI-2 family proteins. J. Cell Mol. Med., 7, 249-257 https://doi.org/10.1111/j.1582-4934.2003.tb00225.x
  2. Fioravanti, L., Miodini, P., Cappelletti, V. and DiFronzo, G. (1998): Synthetic analogs of vitamin D3 have inhibitory effects on breast cancer cell lines. Anticancer Res., 18, 1703-1708
  3. Gaido, K.W., Leonard, L.S., Lovell, S., Gould, J.C., Babai, D., Protier, C.J. and McDonnell, D.P. (1997): Evaluation of chemicals with endocrine modulating activity in a yeastbased steroid hormone receptor gene transcription assay. Toxicol. Appl. Pharmacol., 143, 205-212 https://doi.org/10.1006/taap.1996.8069
  4. Golden, R.J., Noller, K.L., T1tus-Ernstroff, L., Koufman, R.H., Mitterndof, R., Stillman, R. and Reese, E.A. (1998): Environmental endocrine modulators and human health: An assessment of the biological evidence. Crit. Rev. Toxicol., 28, 109-227 https://doi.org/10.1080/10408449891344191
  5. Kang, K.S., Kim, H.S., Ryu, D.Y., Che, J.H. and Lee, Y.S. (2000): Immature uterotrophic assay is more sensitive than ovariectomized uterotrophic assay for the detection of estrogenicity of p-nonylphenol in Sprague-Dawley rats. Toxicol. Lett., 118, 109-115 https://doi.org/10.1016/S0378-4274(00)00272-1
  6. Kim, J.C., Lim, K.H., Suh, J.E., Wee, J.J., Nam, K.Y. and Chung, M.K. (2001): Effect of korean red ginseng water extract on bisphenol A-induced developmental toxicity in rats. J. Toxicol. Pub. Health, 17, 225-234
  7. Milligan, S.R., Khan, O. and Nash, M. (1998): Competitive binding of xenobiotic oestrogen to rat alpha-fetoprotein and to sex steroid binding proteins in human and rainbow trout (Oncorhynchus mykiss) plasma. Gen. Com. Endocrinol., 112, 89-95 https://doi.org/10.1006/gcen.1998.7146
  8. Park, H.J., Lee, M.S., Lee, K.T., Sohn, I.C., Han, Y.N. and Miyamoto, K. (1999): Studies on constituents with cytotoxic activity from the stem bark of Syringa velutina. Chem. Pharm. Bull., 47, 1029-1031 https://doi.org/10.1248/cpb.47.1029
  9. Schafer, T.E., Lapp, C.A., Hanes, C.M., Lewis, J.B., Wataha, J.C. and Schuster, G.S. (1999): Estrogenicity of bisphenol A and bisphenol A dimethacrylate in vitro. J. Biomed. Mater. Res., 45, 192-197 https://doi.org/10.1002/(SICI)1097-4636(19990605)45:3<192::AID-JBM5>3.0.CO;2-A
  10. Simbulan-Rosenthal, C.M., Rosenthal, D.S., Iyer, S., Boulares, H. and Smulson, M.E. (1999): Involvement of PARP and poly(ADP-ribosyl)ation in the early stages of apoptosis and DNA replication. Mol. Cell Biochem., 193, 137-148 https://doi.org/10.1023/A:1006988832729
  11. Soto, A.M., Justica, H., Wray, J.W. and Sonnenschein, C. (1991): p-Nonylphenol: An estrogenic xenobiotic released from 'modified' polystyrene. Environ. Health Perspect, 92, 167-173 https://doi.org/10.2307/3431154
  12. Yamasaki, K., Sawaki, M. and Takatsuki, M. (2000): Immature rat uterotrophic assay of bisphenol A. Environ. Health Perspect., 108, 1147-1150 https://doi.org/10.2307/3434826
  13. Yang, S.R., Hong, H.D., Ahn, N.S., Jung, J.W., Park, J.S., Jo, E.H., Lee, Y.S. and Kang, K.S. (in process): Assessment of hormonal activities using recombinant yeast assay system containing estrogen receptor or androgen receptor and inhibition of MCF-7 cells from plants, foods and herbs. J. Microbio. Biotech.
  14. 식품의약품안전청 (1999): 의약품 등의 독성시험기준, 식품의약품안전청 고시 제 1999-61호
  15. 식품의약품안전청 (2000): 비임상시험기준, 식품의약품안정청 고시 제 2000-63호