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Protective Effect of the Stem Bark of Syringa velutina on Bisphenol-A in the Human Breast Cancer Cell Line and Immature Rat  

Jo Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Yang Se-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Cho Sung-Dae (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Jung Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Park Joon-Suk (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Hwang Jae-Woong (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Lee Seong-Hun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Park Jung-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Lee Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Kang Kyung-Sun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Publication Information
Toxicological Research / v.21, no.1, 2005 , pp. 31-37 More about this Journal
Abstract
The inhibitory activity against bisphenol-A (BPA), one of well-known endocrine disrupters was examined with the water extracts prepared from the Stem Bark of Syringa velutina (SBS). In this study, we have investigated the effect of SBS on the toxicity caused by BPA in human breast cancer cell line, MCF-7 cells and immature Sprague-Dawley rats. In the estrogen receptor-mediated proliferation assay using MCF-7 cells, BPA (16 ng/ml) induced the cell proliferation, but the water extract of SBS inhibited BPA-induced cell proliferation in a dose-dependent manner. These results are associated with PARP degradation and specific cleavage of anti-apoptotic protein Bcl-2 of apoptotic regulatory factors. Additionally, the BPA (400 mg/100 g) significantly induced the increase of the uterine and virginal weights, while SBS (50 mg/100 g) showed the inhibitory action against BPA, i.e. caused the increase of estrogen-related organ weights in immature rat uterotrophic assay. Taken together, the present data suggest that SBS may have anti-toxicity activities against BPA in vitro and in vivo systems. SBS may be capable of inhibiting adverse effects of BPA such as reproductive disorder.
Keywords
Bisphenol-A; MCF-7 cells; Syringa velutina; Uterotrophic assay; PARP; Bcl-2;
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