Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a New ${\gamma}$-Polyglutamic Acid Producer, Bacillus mesentericus MJM1, from Korean Domestic Chungkukjang Bean Paste

  • ZHAO , XIN-QING (Institute of Bioscience and Biotechnology and Department of Biological Science, Myong Ji University) ;
  • PARK, KWAN-HYONG (Institute of Bioscience and Biotechnology and Department of Biological Science, Myong Ji University) ;
  • JIN, YING-YU (Institute of Bioscience and Biotechnology and Department of Biological Science, Myong Ji University) ;
  • LEE, IN HYUNG (Food and Life Science Major, School of Techno Science, Kookmin University) ;
  • YANG, YOUNG-YELL (Institute of Bioscience and Biotechnology and Department of Biological Science, Myong Ji University) ;
  • JOO-WON SUH, (Institute of Bioscience and Biotechnology and Department of Biological Science, Myong Ji University)
  • Published : 2005.02.01
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Abstract

Poly-${\gamma}$-glutamic acid (PGA) is an unusual anionic polypeptide and has great potential as an environmentally and industrially significant biodegradable material. A new ${\gamma}$-PGA producer, Bacillus mesentericus MJM1, with high production capacity was isolated from Korean domestic Chungkuckjang bean paste. It produced ${\gamma}$-PGA at the level of 10 g/l in suitable media. The viscosities of 5% initially extracted mucin and purified ${\gamma}$-PGA solutions were 660 cps and 600 cps, respectively. The produced ${\gamma}$-PGA polymer consisted of 2,000 glutamic acid residues with even proportion of L and D types with molecular mass of about 200- 300 kDa. Bacillus mesentericus MJM1 displayed ${\gamma}$-glutamyltranspeptidase (${\gamma}$-GTP) activity that is known to play a key role in ${\gamma}$-PGA biosynthesis. The ${\gamma}$-GTP coding region was located on the plasmid of 5.8 kb. The plasmid, named pMMH1, is a rolling-circle replication (RCR) plasmid and additionally contained a replication origin and type I signal peptidase (sipP) coding region.

Keywords

References

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