Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Combination Effects of 7β-Hydroxycholesterol and Radiation in Human Lung Cancer Cells

  • KANG Kyoung Ah (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • LEE Kyoung Hwa (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • CHAE Sungwook (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • KIM Dae Yong (Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • PARK Moon Taek (Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • LEE Su Jae (Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • LEE Yun Sil (Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • HYUN Jin Won (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
  • Published : 2005.12.01
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Abstract

The present study was performed to evaluate combination effect of 7$\beta$-hydroxycholesterol (7$\beta$-OHC) and $\gamma$-radiation in NCI-H460 human lung cancer cells. 7$\beta$-OHC in combination with $\gamma$-irradiation has an enhanced effect in decreasing clonogenic survival and increasing cellular DNA damage. Pretreatment of cells with 7$\beta$-OHC enhanced radiation-induced apoptosis. Apoptosis of the cells by combined treatment of 7$\beta$-OHC and $\gamma$-irradiation was associated with reactive oxygen species generation and loss of mitochondrial membrane potential, resulting in the activation of caspase 9 and caspase 3. The combined treatment also resulted in an increased G1 cell cycle distribution. These results indicate that 7$\beta$-OHC shows the additive effect of radiation sensitivity in human lung carcinoma cells in vitro.

Keywords

References

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