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Combination Effects of 7β-Hydroxycholesterol and Radiation in Human Lung Cancer Cells  

KANG Kyoung Ah (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
LEE Kyoung Hwa (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
CHAE Sungwook (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
KIM Dae Yong (Laboratory of Radiation Effect, Korea Cancer Center Hospital)
PARK Moon Taek (Laboratory of Radiation Effect, Korea Cancer Center Hospital)
LEE Su Jae (Laboratory of Radiation Effect, Korea Cancer Center Hospital)
LEE Yun Sil (Laboratory of Radiation Effect, Korea Cancer Center Hospital)
HYUN Jin Won (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Publication Information
Biomolecules & Therapeutics / v.13, no.4, 2005 , pp. 220-226 More about this Journal
Abstract
The present study was performed to evaluate combination effect of 7$\beta$-hydroxycholesterol (7$\beta$-OHC) and $\gamma$-radiation in NCI-H460 human lung cancer cells. 7$\beta$-OHC in combination with $\gamma$-irradiation has an enhanced effect in decreasing clonogenic survival and increasing cellular DNA damage. Pretreatment of cells with 7$\beta$-OHC enhanced radiation-induced apoptosis. Apoptosis of the cells by combined treatment of 7$\beta$-OHC and $\gamma$-irradiation was associated with reactive oxygen species generation and loss of mitochondrial membrane potential, resulting in the activation of caspase 9 and caspase 3. The combined treatment also resulted in an increased G1 cell cycle distribution. These results indicate that 7$\beta$-OHC shows the additive effect of radiation sensitivity in human lung carcinoma cells in vitro.
Keywords
radiation sensitivity; NCI-H460 human lung cancer cells;
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