The Antioxidant Effect of Lactobacillus gasseri KACC 91155 Isolated from Korean Infant in Jurkat T Cells

유아의 분변에서 분리한 Lactobacillus gasseri KACC 91155의 Jurkat T Cells에서 항산화 효과

  • Published : 2005.12.01

Abstract

In the present study, we investigate the protective effect of antioxidant strain Lactobacillus gasseri KACC 91155, isolated from Korean infant feces(Obstetrics & Gynecology, Suwon, Korea) on the oxidative stress damage on the Jurkat T cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA(malondialdehyde) production was measured Furthermore, cell viability was detected by the MTT assay, DNA damage was tested by the comet assay. Cell grown in medium with or without L gasseri lysate$(100\~1,000{\mu}g)$ were treated with $H_2O_2,\;Fe^{2+}$ as an oxidative stimulus. From the result obtained, the supplementation of Jurkat T cells with L. gasseri lysate significantly decreased in MDA production (1,250 vs. 835 nmol/mg protein), and DNA damage(31.6 vs. 22.6 tail moment). Also L gasseri increase cell viability against oxidative damage. We concluded that the L. gasseri KACC 91155 showed a protective effect against oxidative stress.

본 연구에서는 유아의 분변에서 분리한 항산화 효과를 갖는 유산균인 Lactobacillus gasseri KACC 91155의 Jurkat T cell line에서의 지질 과산화 억제 효과, 세포 보호 효과 및 DNA 손상 억제 효과를 측정하였다. 그 결과 1. gasseri 91155는 세포 산화에 의해 생성된 지질 과산화물인 MDA의 생성을 방지하였으며, 또한 뚜렷하게 세포 생존성을 증가시켰다. 그리고 산화적 손상으로부터 DNA 손상을 줄여주는 효과를 나타내었다.

Keywords

References

  1. Annuk, H., Shchepetova, J., Kullisaar, T., Songisepp, E., Zilmer, M., and Mikelsaar, M. (2003) Characterization of intestinal lactobacilli as putative probiotic candidates. J. Appl. Microbiol. 94, 403-412 https://doi.org/10.1046/j.1365-2672.2003.01847.x
  2. Aruoma, O. I. (1996) Characterization of drugs as antioxidant prophylactics. Free Radical Bio. Med. 20, 671-683
  3. Bonnes-Taourei, D., Guerin, M. C., and Torreilles, J. (1992) Is malondialdehyde a valuable indicatior of lipid peroxidation. Biochem. Pharmacol. 44, 985-988 https://doi.org/10.1016/0006-2952(92)90132-3
  4. Cantoni, O., Cattabeni, F., Stocchi, V., Meyn, R. E., Cerutti, P., and Murray, D. (1989) Hydrogen peroxide insult in cultures mammalian cells; relationship between DNA single-strand breakage, poly(ADP-ribose) metabolism, and cell killing. Biochem. Biophys. Acta. 1014, 1-7 https://doi.org/10.1016/0167-4889(89)90234-6
  5. Chandra, J., Samali, A., and Orrenius, S. (2000) Triggering and modulation of apoptosis by oxidative stress. Free Radic. Biol. Med. 29, 323-333 https://doi.org/10.1016/S0891-5849(00)00302-6
  6. Davies, K. J. A. (1995) Oxidative stress: the paradox of aerobic life. Biochemical Society Symposium No. 61. London. pp. 1-35
  7. Duthie, S. J., Ma, A., Ross, M. A., and Collins, A. R. (1996) Antioxidant supplementation decrease oxidative DNA damage in human lymphocytes. Cancer Res. 56, 1291-1295
  8. Erba, D., Riso, P., Criscuoli, F., and Testolin, G. (2003) Malondialdehyde production in Jurkat T cells subjected to oxidative stress. Nutr. 19, 545-548 https://doi.org/10.1016/S0899-9007(02)01010-9
  9. Finkel, T. (2000) Redox-dependent singnal transduction. FEBS Lett. 476, 52-54 https://doi.org/10.1016/S0014-5793(00)01669-0
  10. Halliwell, B. (1991) Drug antioxidant effects: a basis for drug selection. Drugs. 42, 569-605 https://doi.org/10.2165/00003495-199142040-00003
  11. Halliwell, B. and Chirico, S. (1993) Lipid peroxidation: its mechanism, measurement, and significance. Am. J. Clin. Nutr. 57, 715-724
  12. Halliwell, B., Clement, M. V., and Long, L. H. (2000) Hydrogen peroxide in the human body. FEBS Lett. 486, 10-13 https://doi.org/10.1016/S0014-5793(00)02197-9
  13. Han, Y. T., Han, Z. W., Yu, G. Y., Wang, Y. J. Cui, R. Y. and Wang, C. B. (2004) Inhibitory effect of polypeptide from Chlamys farreri on ultraviolet A-induced oxidative damage on human skin fibroblasts in vitro. Pharmacol. Res. 49, 265-274 https://doi.org/10.1016/j.phrs.2003.09.009
  14. Humpries, K. M. and Sweda, L. I. (1998). Selective inactivation of ${\alpha}$-ketoglutarate dehydrogenase: reaction of lipoic acid with 4-hydroxy-2-nonenal. Biochem. 37, 15835-15841 https://doi.org/10.1021/bi981512h
  15. Kim, H. S., Chae, H. S., Jeong, S. G., Ham, J. S., Im, S. K., Ahn, J. N., and Lee, J. M. (2005) Antioxidative activity of some yogurt starter cultures. Asian-Aust. J. Anim. Sci. 18, 255-258
  16. Konicova, E., Babusikova, O., Kusenda, J., and Glasova, M. (1992) Detection of cytoplasmatic and surface membrane markers in cells of some human hematopoietic cell lines. Neoplasma. 39, 337-342
  17. Kullisaar, T., Zilmer, M., Mikelsaar, M., Vihalemm, T., Annuk, H., Kairane, C., and Kilk, A. (2002) Two antioxidative lactobacilli strains as promising probiotics. Intl. Food Microbiol. 72, 215-224. https://doi.org/10.1016/S0168-1605(01)00674-2
  18. Li, L. and Lau, B. H. (1993) A simplified in vitro model of oxidant injury using vascular endothelial cells. In Virto Cell Dev. Biol. Anim. 29, 531-536 https://doi.org/10.1007/BF02634146
  19. Lin, M. Y. and Chang, F. J. (2000) Antioxidative effect of intestinal bacteria Bifidobactenum longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356. Digestive Diease Sci. 45, 1617-1622 https://doi.org/10.1023/A:1005577330695
  20. Riso, P., Erba, D., Criscuoli, F., and Testolin, G. (2002) Effect of green tea extract on DNA repair and oxidative damage due to $H_{2}O_{2}$ in Jurkat T cell. Nurt. Res. 22, 1143-1150
  21. Riso, P., Santangelo, A., and Porrini, M. (1999) The comet assay for the evaluation of cell resistance to oxidative stress. Nurt. Res. 19, 325-333
  22. Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. (1998) A simple technique for quantitation of low levels of DNA damage in individuals cells. Exp. Cell. Res. 175, 184-191
  23. Tanaka, K. I., Fujita, N., and Yoshioka, M. (2001) Immunosuppressive and non-immunosuppressive immunophilin ligands improve $H_{2}O_{2}$-induced cell damage by increasing glutathione levels in NG108-15 cells. Brain Res. 889, 225-228
  24. Thannickal, V. J. and Fanburg, B. L. (2000) Reactive oxygen species in cell signaling. Am. J. Physiol. Lung Cell Mol. Physiol. 279, 11005-11028
  25. Uchida, K. (2000) Role of active aldehyde in cardiovascular disease. Free Radic. Biol. Med. 28. 1685-1696 https://doi.org/10.1016/S0891-5849(00)00226-4
  26. Wang, L., Nishida, H., Ogawa, Y., and Konishi, T. (2003) Prevention of oxidative injury in PC12 cells by a traditional Chinese medicine, Shengmai San, as a model of an antioxidant-based composite formula. Biol. Pharm. Bull. 26, 1000-1004 https://doi.org/10.1248/bpb.26.1000
  27. Xiao, X. O., Lee, N. T. K., Carlier, P. R., Pang, Y. P., and Han, Y. F. (2000) Bis(7)-tacrine-induced injury in rat pheochromocytoma cells: comparison with tacrine. Neurosci. Lett. 209, 197-200 https://doi.org/10.1016/0304-3940(96)12646-X