Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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RPK118, a PX Domain-containing Protein, Interacts with Peroxiredoxin-3 through Pseudo-Kinase Domains

  • Liu, Lungling (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University) ;
  • Yang, Chenyi (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University) ;
  • Yuan, Jian (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University) ;
  • Chen, Xiujuan (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University) ;
  • Xu, Jianing (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University) ;
  • Wei, Youheng (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University) ;
  • Yang, Jingchun (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University) ;
  • Lin, Gang (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University) ;
  • Yu, Long (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
  • Received : 2004.08.12
  • Accepted : 2004.09.15
  • Published : 2005.02.28
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Abstract

RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed by pull-down assays and co-immunoprecipitation experiments. Deletion studies showed that RPK118 interacted with PRDX3 through its pseudokinase domains, and with early endosomes through its PX domain. Double immunofluorescence experiments demonstrated that PRDX3 co-localized with RPK118 on early endosomes in COS7 cells. PRDX3 is a member of the antioxidant family of proteins synthesized in the cytoplasm and functioning in mitochondria. Our findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other membrane structures via endosome trafficking.

Keywords

References

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