식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제35권2호
  • /
  • Pages.99-114
  • /
  • 2005
  • /
  • 1225-8318(pISSN)
  • /
  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
권호 표지

한반도 고유종 큰꽃땅비싸리의 배수성과 종분화

Polyploidy and speciation in Korean endemic species of Indigofera grandiflora (Leguminosae)

  • 김원희 (인하대학교 자연과학대학 생명과학과) ;
  • 김소영 (인하대학교 자연과학대학 생명과학과) ;
  • 최병희 (인하대학교 자연과학대학 생명과학과)
  • 투고 : 2005.01.27
  • 심사 : 2005.06.10
  • 발행 : 2005.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

한국산 땅비싸리속 식물의 종분화를 연구하기위해 염색체수, 형태적변이 및 RAPD분석을 실시하였다. 땅비싸리(2n=16) 와 좀땅비싸리(2n=32)의 염색체수는 기존의 보고와 일치하였다. 본 연구에서 큰꽃땅비싸리의 4배체(2n=32)와 6배체(2n=48)집단이 새로 조사되었다. 큰꽃땅비싸리는 가야산 부근에서 땅비싸리와 좀땅비싸리와 함께 자라고 있었다. 큰꽃땅비싸리는 이들 두 종에 비해 식물체 높이, 잎 및 꽃이 더 크며, 땅비싸리 근연종에서 중요한 분류형질인 잎과 꽃의 털에서 땅비싸리와 좀땅비싸리의 중간 형태를 보이고 있다. RAPD분석에서 큰꽃땅비싸리는 좀땅비싸리와 더 유사하였으나, 4배체와 6배체 간에 차이를 보였다. 이러한 결과는 한반도 고유종인 큰꽃땅비싸리가 공동분포지인 가야산 지역에서 땅비싸리와 좀땅비싸리 사이의 잡종현상과 함께 배수체현상에 의해 기원된 것으로 사료된다.

Chromosome number, morphological variation and RAPD analysis were investigated to study on the speciation of Indigofera in Korea. Chromosome numbers of I. kirilowii (2n=16) and I. koreana (2n=32) are consistent with the previous reports. In this study tetraploid (2n=32) and hexaploid (2n=48) of I. grandiflora are newly observed. Indigofera grandiflora is distributed around Mt. Kaya area together with I. kirilowii and I. koreana. The former species has the larger sizes in plant height, leaves and flowers than the latter two and shows intermediate form between the two species in hairs on leaves and flowers which are one of the most important taxonomic characters in this group. In the RAPD analysis, I. grandiflora is similar to I. koreana than I. kirilowii but RAPD band patterns revealed difference between tetra- and hexaploid of the species. These results suggested that Korean endemic species of I. grandiflora (2n=16, 32, 48) might has multiple origin through polyploidization and/or hybridization between I. kirilowii (2n=16) and I. koreana (2n=32) around Mt. Kaya area where the latter two grow together.

키워드

참고문헌

  1. Caraway, V., G. D. Carr and C. W. Morden. 2001. Assessment of hybridization and introgression in lava-colonizing Hawaiian Dubautia (Asteraceae: Madiinae) using RAPD markers. Amer. J. Bot. 88: 1688-1694 https://doi.org/10.2307/3558414
  2. Cho, S. K. 1995. A taxonomic study of Indigofera kirilowii and its related species (Leguminosae) in East Asia. Master's thesis of Inha Univ. (In Korean)
  3. Cho, S. K, B. H. Choi and J. H. Kim. 1997. Morphological variations and taxonomy of Indigofera kirilowii and I koreana (Leguminosae). Korean J. Pl. Taxon. 27: 73-87. (In Korean)
  4. Choi, B. H. 1996. Indigofera grandiflora (Leguminosae), a new species from Korea. J. Plant Biol. 39: 145-148
  5. Choi, B. H. and J. H. Kim. 1997. ITS sequences and speciation on Far Eastern Indigofera (Leguminosae). J. Plant Res. 110: 339-346 https://doi.org/10.1007/BF02524932
  6. Cook, L. M., P. S. Soltis, S. J. Brunsfeld and D. E. Soltis. 1998. Multiple independent formations of Tragopogon tetraploids (Asteraceae): evidence from RAPD markers. Molecular Ecology 7: 1293-1302 https://doi.org/10.1046/j.1365-294x.1998.00453.x
  7. Dice, L. R. 1945. Measures of the amount of ecological association between species. Ecology 26: 295-302
  8. Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochern. Bull. 19: 11- 15
  9. Fang, Y.-Y. and C.-Z. Zheng, 1989. A study on the genus Indigofera Linn. from China. Acta Phytotax. Sin. 27: 161-177
  10. Gonzalez-Perez, M. A, J. Caujape-Castells and P. A Sosa. 2004. Molecular evidence of hybridization between the endemic Phoenix canariensis and the widespread P. dactylifera with Random Amplified Polymorphic DNA(RAPD) markers. Plant Syst, Evol. 247: 165-175
  11. Grant, V. 1963. The Origin of Adaptations. Columbia Univ. Press, New York
  12. Grant, V. 1981. Plant Speciation (2nd ed.). Columbia Univ. Press, New York
  13. Kawakami, I. 1930. Chromosome numbers in Leguminosae. Bot. Mag. (Tokyo) 44: 319-328 https://doi.org/10.15281/jplantres1887.44.319
  14. Kodama, A 1967. A cytological studies on root nodules of some species in Leguminosae 2. Bot. Mag. (Tokyo) 80: 92-99 https://doi.org/10.15281/jplantres1887.80.92
  15. Koontz, J. A, P. S. Soltis and S. J. Brunsfeld. 2001. Genetic diversity and tests of the hybrid origin of the endangered yellow larkspur. Conserv. Biol. 15: 1608-1618 https://doi.org/10.1046/j.1523-1739.2001.00098.x
  16. Kwon, S. K and B. H. Choi. 2001. Taxonomy of Korean endemic species of Vida hirticalycina based on morphological and genetic variations. Korean J. PI. Taxon. 31: 15-31. (In Korean)
  17. Levin, D. A 1983. Polyploidy and novelty in flowering plants. Am. Nat. 122: 1-25 https://doi.org/10.1086/284115
  18. Luo, M. C, K. K. Hwu and T. C. Huang. 2000. Taxonomic study of Taiwan Astragalus based on genetic variation. Taxon 49: 35-46 https://doi.org/10.2307/1223930
  19. Neuffer, B., H. Auge, H. Mesch, U. Amarell and R. Brandl. 1999. Spread of violets in polluted pine forests: morphological and molecular evidence for the ecological importance of interspecific hybridization. Mol. Ecol. 8: 365-377 https://doi.org/10.1046/j.1365-294X.1998.00566.x
  20. Otto, S. P. and J. Whitton, 2000. Polyploid incidence and evolution. Ann. Rev. Genet. 34: 401-437 https://doi.org/10.1146/annurev.genet.34.1.401
  21. Potokina, E., N. Tomooka, D. A Vaughan, T. Alexandrova and R.-Q. Xu. 1999. Phylogeny of Vicia subgenus Vicia (Fabaceae) based on analysis of RAPDs and RFLP of PCR-amplified chloroplast genes. Gen. Res. Crop Evol. 46: 149-161 https://doi.org/10.1023/A:1008640322198
  22. Rost, T. L., M. G. Barbour, C. R. Stocking and T. M. Murphy. 1998. Plant Biology. Wadsworth Publishing Company, California. Pp, 274
  23. Schrire, B. D. 1995. Evolution of the tribe Indigofera (Leguminosae-Papilionoideae). In Advance in Legume Systematics, Part 7: Phylogeny. M. D. Crisp & J. J. Doyle (eds.), Royal Botanic Gardens, Kew. Pp. 161-244
  24. Segraves, K. A, J. N. Thompson, P. S. Soltis and D. E. Soltis. 1999. Multiple origins of polyploidy and the geographic structure of Heuchera grossulariifolia. Molecular Ecology. 8: 253-262 https://doi.org/10.1046/j.1365-294X.1999.00562.x
  25. Seok, D. I. and B. H. Choi. 1998. Taxonomic relationships in East Asian Vicia species with unijugate leaves based on random amplified polymorphic DNA markers. J. Plant Biol. 41: 201-207 https://doi.org/10.1007/BF03030254
  26. Shim, H. B. and B. H. Choi. 2004. RAPD marker variations between and within the species of Korean Suaeda. Korean J. PI. Taxon. 34: 63-74. (In Korean)
  27. Soltis, D. E. and P. S. Soltis. 1993. Molecular data and the dynamic nature of polyploidy. Crit. Rev. Plant Sci. 12: 243-273 https://doi.org/10.1080/713608048
  28. Soltis, D. E. and P. S. Soltis. 1999. Polyploid: recurrent formation and genome evolution. Tree 14: 348-352
  29. Soltis, P. S., J. J. Doyle and D. E. Soltis. 1992. Molecular data and polyploid evolution in plant. In Molecular Systematics of Plants. Soltis, P. S., J. J. Doyle and D. E. Soltis (eds.), Chapman and Hall, New York. Pp. 177-201
  30. Soltis, P. S, G. M. Plunkett, S. J. Novak and D. E. Soltis. 1995. Genetic variation in Tragopogon species: additional origins of the allotetraploids T mirus and T miscellus (Compositae). Amer. J. Bot. 82: 1329-1341 https://doi.org/10.2307/2446255
  31. Stebbins, G. L. 1971. Chromosomal Evolution in Higher Plants. Addison-Wesley publishing. Massachusetts
  32. Sugiura, T. 1931. A list of chromosome numbers in angiospermous plant. Bot. Mag. (Tokyo) 45: 353-355 https://doi.org/10.15281/jplantres1887.45.353
  33. Theseng, F. S., T. K. Lin and S. T. Wu. 2000. Relations of genus Glycine subgenus Soja and Glycine formosana Hosok. collected from Taiwan: Revealed by RAPD analysis. J. Jap. Bot. 75: 270-279
  34. Urbanska, K. M., H. Hurka, E. Landolt, B. Neuffer and K. Mummenhoff. 1997 Hybridization and evolution in Cardamine (Brassicaceae) at Umerboden Central Switzerland: biosystematic and molecular evidence. Plant Syst. Evol. 20: 233-256
  35. Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful genetic markers. Nucl. Acid Res. 18: 6531-6535 https://doi.org/10.1093/nar/18.22.6531