Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Morphological Characteristics and Pathogenicity of Streptococcus iniae

Streptococcus iniae의 형태학적 특성과 병원성

  • Kim, Hyun-Jeong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Woo, Sung-Ho (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Jin-Woo (Pathology Division, NFRDI) ;
  • Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 김현정 (부경대학교 수산생명의학과) ;
  • 우승호 (부경대학교 수산생명의학과) ;
  • 김진우 (국립수산과학원 병리연구팀) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Published : 20050800
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Abstract

Streptococcus iniae is one of the major bacterial pathogens of cultured olive flounder, Paralichthys olivaceus in Korea. This study was carried out to investigate the interaction between morphological characteristics and immune response such as phagocytosis and serum killing activity. They were differentiated phenotypically into two groups, the viscous colonies and the non-viscous colonies, but the strains in both groups were similar in physiological and biochemical characteristics. Electron microscopic examination of the viscous form revealed thick, electron-dense exopolysaccharide materials surrounding polycationic ferritin-stained cells, while the exopolysaccharide material was absent around the non-viscous form of S. iniae. The viscous type strains were disclosed more virulent than those of non-viscous type for olive flounder. The viscous strains were resistant to normal serum killing activity, while the non-viscous strains were susceptible to bactericidal activity of normal serum from olive flounder. The viscous strains were more resistant to opsonophagocytosis and decreased the chemiluminescence response of head kidney macrophages of olive flounder. All of the tested non-viscous S. iniae strains were efficiently destroyed by the macrophage after phagocytosis. Both the viscous and the non-viscous strains invaded and replicated in cultured fish cell line (CHSE-2l4). This cellular invasion may contribute to the pathogenesis of invasive S. iniae infection.

본 연구에서는 S. iniae의 병원성 요인으로 작용할 수 있는 capsule의 존재 여부를 확인하고 그에 따른 capsule과 병원성과의 상관 관계를 조사하였다. 즉, 집락의 형태학적 특성에 따라 viscous form (V+)과 non-viscous form (V-)의 균주로 구분하여 그 특성을 비교 분석하였다. 분리균의 생리학적 및 생화학적 특성으로는 숙주의 방어 기작에 대한 저항성이나 병원성 차이를 구분할 수 없었으나 형태학적으로 큰 차이를 있음을 알 수 있었다. 즉, V+인 경우 cell wall 표면에 capsule이 존재하지만 V-에서는 capsule이 존재하지 않는 것으로 확인되었다. V+는 넙치 혈청에서 증식할 수 있지만 V-는 넙치 혈청에 의해 살균되는 것으로 나타나 capsule의 유무에 따라 넙치의 체액성 면역 요소에 대한 저항력에서 차이가 나는 것을 알 수 있었다. 또한, V+는 넙치 신선 혈청으로 감작한 경우에도 V-에 비해 macrophage의 식균율이 낮았으며 식균된 이후에는 균 수가 지속적으로 감소하는 것을 확인할 수 있었고 일반 어류 주화 세포 내로 침입하여 생존 및 증식할 수 있는 것으로 나타났다. 이들 결과는 앞으로의 백신 개발에 유용한 기초 자료로 사용될 수 있을 것으로 사료되었다.

Keywords

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