Enterobacter cloacae YJ-1의 고정화세포에 의한 과일 폐기물로부터 수소생산

Hydrogen Production from Fruit Wastes by Immobilized Cells of Enterobacter cloacae VJ-1

본 연구에서는 과일 폐액으로부터 고정화 세포를 이용하여 수소생산을 위한 배양조건을 조사하였다. 각종 과일 폐액 중에 수박 폐액에서 환원당의 함량이 가장 높았으며, 수소생산량은 2319.2 mL/L이었다. 고정화 물질 sodium alginate의 농도와 크기에 따른 수소생산성 효과는 검토범위 내에서는 적었다. 고정화된 비드의 내부 관찰에서 세포가 왕성하게 생육하고 있음을 확인했다. 대사의 효소물질로 이용될 수 있는 각종 아미노산의 첨가는 종류에 관계없이 수소생산성에 영향을 거의 미치지 않았다. 금속이온 $FeSO_4$를 첨가한 결과 최적 농도는 1.2 g/L이고, 1.3배의 수소생산 증가를 나타났다. 수소생산정지 후 배양액의 유기산은 lactic acid와 butyic acid가 가장 많았다.

The hydrogen production using immobilized cellsl was conducted using fruit wastewaters at various culture conditions. Three kinds of fruit wastewaters, melon, watermelon and pear were used. Sodium alginate was used as immobilization material. Among them, concentration of reducing sugar which was one of the main components in fruit was the highest at watermelon wastewater, and also hydrogen production was the highest as 2319.2 mL/L in it. Although hydrogen production was not much changed according to sodium alginate concentration, its production was the most at 3%(w/v). As bead size as small, hydrogen production was higher. With inspection of interior, it confirmed that the cell grew well in bead. But the addition of amino acids using as agent for metabolite production had almost no affected on hydrogen productivity. The effective range of $FeSO_4$ addition on hydrogen production were up to 1.2 g/L, and above the concentration, it inhibited the productivity. Organic acids produced during watermelon fermentation were mainly lactic acid, butyric acid, abd acetic acid; and a little of propionic acid.