Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Degradation of Crystalline Cellulose by the Brown-rot Basidiomycete Fomitopsis palustris

  • Yoon Jeong-Jun (Department of Forest Products, College of Forest Science, Kookmin University) ;
  • Kim Young-Kyoon (Department of Forest Products, College of Forest Science, Kookmin University)
  • Published : 2005.12.01
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Abstract

This study demonstrated that the brown rot basidiomycete Fomitopsis palustris was able to degrade crystalline cellulose (Avicel). This fungus could also produce the three major cellulases (exoglucanases, endoglucanases, and $\beta-glucosidase$) when the cells were grown on $2.0\%$ Avicel. Avicel degraded by F. palustris showed a decrease in relative crystallinity from $83\%\;to\;78.5\%$ after 14 days of incubation. The characterization study indicated that optimum pH was 4.5 and optimum temperature was $70^{\circ}C$ for exoglucanase (cellobiohydrolase) activity. Hydrolysis of Avicel by the crude enzyme from F. palustris yielded 1.6 mg/ml of glucose after 43 h, which corresponded to a cellulose conversion degree of $3.2\%$. Therefore, this study revealed for the first time that the brown rot basidiomycete F. palustris produces cellulases capable of yielding soluble sugars from crystalline cellulose.

Keywords

References

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