Archives of Pharmacal Research

  • 제28권11호
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  • Pages.1244-1250
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  • 2005
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Inhibitory Effects of Eutigosides Isolated from Eurya emarginata on the Inflammatory Mediators in RAW264.7 Cells

  • Park Soo-Yeong (Biodiversity Research Institute, Jeju HiDI) ;
  • Lee Hye-Ja (Department of pharmacology, College of Medicine, Cheju National University) ;
  • Yoon Weon-Jong (Department of pharmacology, College of Medicine, Cheju National University) ;
  • Kang Gyoung-Jin (Department of pharmacology, College of Medicine, Cheju National University) ;
  • Moon Ji-Young (Department of Chemistry, College of Natural Science, Cheju National University) ;
  • Lee Nam-Ho (Department of Chemistry, College of Natural Science, Cheju National University) ;
  • Kim Se-Jae (Technology Innovation Center for Life Science, Cheju National University, Cheju National University) ;
  • Kang Hee-Kyoung (Department of pharmacology, College of Medicine, Cheju National University) ;
  • Yoo Eun-Sook (Department of pharmacology, College of Medicine, Cheju National University)
  • 발행 : 2005.11.01
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초록

The anti-inflammatory activity of Eurya emarginata (Thumb) Makino, of which leaves have been traditionally used to treat ulcers or diuretic in Jeju Island, has been investigated in the present study. Through the phytochemical study from the methanol extract of E. emaginiata, eutigosides Band C were isolated as the active components. Sseveral inflammatory markers including TNF-$\alpha$, IL-1$\beta$, IL-6, NO, iNOS, and COX-2 were examined. Eutigosides Band C potentially inhibited production of pro-inflammatory cytokines (IL-6 and TNF-$\alpha$) in a dose-dependent manner. Additionally, the intracellular contents of iNOS protein were markedly decreased after treatment with eutigosides Band C. The inhibition of iNOS activity was correlated with the decrease in nitrite levels. These results suggest that eutigoside Band C from E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines (TNF-$\alpha$ and IL-6), iNOS and COX-2.

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