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Inhibitory Effects of Eutigosides Isolated from Eurya emarginata on the Inflammatory Mediators in RAW264.7 Cells  

Park Soo-Yeong (Biodiversity Research Institute, Jeju HiDI)
Lee Hye-Ja (Department of pharmacology, College of Medicine, Cheju National University)
Yoon Weon-Jong (Department of pharmacology, College of Medicine, Cheju National University)
Kang Gyoung-Jin (Department of pharmacology, College of Medicine, Cheju National University)
Moon Ji-Young (Department of Chemistry, College of Natural Science, Cheju National University)
Lee Nam-Ho (Department of Chemistry, College of Natural Science, Cheju National University)
Kim Se-Jae (Technology Innovation Center for Life Science, Cheju National University, Cheju National University)
Kang Hee-Kyoung (Department of pharmacology, College of Medicine, Cheju National University)
Yoo Eun-Sook (Department of pharmacology, College of Medicine, Cheju National University)
Publication Information
Archives of Pharmacal Research / v.28, no.11, 2005 , pp. 1244-1250 More about this Journal
Abstract
The anti-inflammatory activity of Eurya emarginata (Thumb) Makino, of which leaves have been traditionally used to treat ulcers or diuretic in Jeju Island, has been investigated in the present study. Through the phytochemical study from the methanol extract of E. emaginiata, eutigosides Band C were isolated as the active components. Sseveral inflammatory markers including TNF-$\alpha$, IL-1$\beta$, IL-6, NO, iNOS, and COX-2 were examined. Eutigosides Band C potentially inhibited production of pro-inflammatory cytokines (IL-6 and TNF-$\alpha$) in a dose-dependent manner. Additionally, the intracellular contents of iNOS protein were markedly decreased after treatment with eutigosides Band C. The inhibition of iNOS activity was correlated with the decrease in nitrite levels. These results suggest that eutigoside Band C from E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines (TNF-$\alpha$ and IL-6), iNOS and COX-2.
Keywords
Eutigoside B; Eutigoside C; Pro-inflammatory cytokines; iNOS; COX-2;
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1 Cho, J. Y., Park, J., Yoo, E. S., Baik, K. U., Lee J., and Park, M. H., Inhibitory effect of sesquiterpene lactones from Saussurea lappa on tumor necrosis factor-$\alpha$ production in murine macrophage-like cell. Planta Med., 64, 594-597 (1998)   DOI   ScienceOn
2 Hersh, D., Weiss, J., and Zychlinsky, A., How bacteria initiate inflammation: aspects of the emerging story. Curr. Opin. Microbiol., 1, 43-48 (1998)   DOI   ScienceOn
3 MacMicking, J., Xie, Q. W., and Nathan, C., Nitric oxide and macrophage function. Annu. Rev. Immunol., 15, 323-350 (1997)   DOI   ScienceOn
4 Marletta, M. A., Yoon, P. S., Iyengar, R., Life, C. D., and Wishnok, J. S., Oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate. Biochemistry, 27, 8706-8711 (1988)   DOI   ScienceOn
5 Marin, J. and Rodriguez-Martinez, M. A., Role of vascular nitric oxide in physiological and pathological conditions. Pharmacol. Therap., 75, 111-134 (1997)   DOI   ScienceOn
6 Moncada, S., Palmer, R. M., and Higgs, E. A., Nitric oxide: physiology, pathophysiology. Pharmacol. Rev., 43, 109-142 (1992)
7 Needleman, P. and Isakson, P. C., Selective inhibition of cyclooxygenase 2. Sci. Med., 1, 35-36 (1998)
8 Oppenheim, J. J., Cytokines: past, present, and future. Int. J. Hematol., 74, 38 (2001)
9 Ryu, S. Y., Oak, M. H., Yoon, S. K., Cho, D. I., Yoo, G. S., Kim, T. S., and Kim, K. M., Anti-allergic and anti-inflammatory triterpenes from the herb of Prunella vulgaris. Planta Medica, 66, 358-360 (2000)   DOI   ScienceOn
10 Rouveix, B., Clinical pharmacology of cytokines. Eur. Cytokine Netw., 8, 291-293 (1997)
11 Xie, Q. W., Kashiwabara, Y., and Nathan, C., Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase. J. Biol. Chem., 69, 4705-4708 (1994)
12 Mukaida, N., Ishikawa, Y., Ikeda, N., Fujioka, N., Watanabe, S., Kuno, K., and Matsushima, K., Novel insight into molecular mechanism of endotoxin shock; biochemical analysis of LPS receptor signaling in a cell-free system targeting NF-kapperB and regulation of cytokine production/action through beta2 integrin in vivo. J. Leukoc. Biol., 59, 145-151 (1996)   DOI
13 Schutt, C. and Schumann, R., The endotoxin recepter CD14. Immun. Infekt., 21, 36-40 (1993)
14 Nicod, L. P., Cytokines: overview. Thorax, 48, 660-667 (1993)   DOI   PUBMED   ScienceOn
15 Grisham, M. B., Granger, D. N., and Lefer, D. J., Modulation of leukocyte-endothelial interactions by reactive metabolites of oxygen and nitrogen: relevance to ischemic heart disease. Free Radical Biol. Med., 25, 404-433 (1998)   DOI   ScienceOn
16 Dinarello, C. A., Pro-inflammatory cytokines. Chest., 118, 503- 508 (2000)   DOI   PUBMED   ScienceOn
17 Turcanu, V. and Williams, N. A., Cell identification and isolation on the basis of cytokine secretion: a novel tool for investigating immune responses. Nat. Med., 7, 373-376 (2001)   DOI   ScienceOn
18 Chung, M. G. and Epperson, B. K., Clonal and spatial genetic structure in Eurya emarginata (Theaceae). Heredity., 84, 170-177 (2000)   DOI   ScienceOn
19 Holloway, A. F., Rao, S., and Shannon, M. F., Regulation of cytokine gene transcription in the immune system. Mol. Immunol., 38, 567-580 (2002)   DOI   ScienceOn
20 Perry, E. K., Pickering, A. T., Wang, W. W., Houghton, P. J., and Perru, N. S., Medicinal plants and Alzheimer's disease: from ethnobotany to phytotherapy. J. Pharm. Pharmacol., 51, 527-534 (1999)   DOI
21 Sambrook, J. E., Fritsch, F., and Maniatis, T., In Molecular Cloning Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, United States of America. 7-8 (1989)
22 Diaz, A., Reginato, A. M., and Jimenez, S. A., Protein, nucleotide alternative splicing of human prostaglandin G/H synthase mRNA and evidence of differential regulation of the resulting transcripts by transforming growth factor beta 1,interleukin 1 beta, and tumor necrosis factor-alpha. J. Biol. Chem., 267, 10816-10822 (1992)
23 Rosselli, M., Keller, P. J., and Dubey, R. K., Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Hum. Reprod. Update., 4, 3-24 (1998)   DOI   ScienceOn
24 Khan, I. A., Erdelmeier, C. A. J., and Sticher, O., New phenolic glucosides from the leaves of Eurya tigang. J. Nat. Prod., 55, 1270-1274 (1992)   DOI   PUBMED
25 Smith, W. L. and Marnett, L. J., Prostaglandin endoperoxide synthase: structure and catalysis. Biochem. Biophys. Acta, 1083, 1-17 (1991)   DOI   ScienceOn
26 Lerner, U. H. and Modeer, T., Bradykinin B1 and B2 receptor agonists synergistically potentiate interleukin-1 induced prostaglandin biosynthesis in human gingival fibroblasts. Inflammation., 15, 427-436 (1991)   DOI   PUBMED
27 Lazarov, S., Balutsov, M., and Ianev, E., The role of bacterial endotoxins, receptors and cytokines in the pathogenesis of septic(endotoxin) shock. Vutr. Boles., 32, 33-40 (2000)
28 Ulevitch, R. J. and Tobias, P. S., Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu. Rev. Immunol., 13, 437-457 (1995)   DOI   ScienceOn
29 Axtelle, T. and Pribble, J., IC14, a CD14 specific monoclonal antibody is a potential treatment for patients with severe sepsis. J. Endotoxin Res., 7, 3599-3605 (2001)
30 Boraschi D., Cifone, M. G., Falk, W., Flad, H. -D., Tagliabue, A., and Martin, M. U., Cytokines in inflammation. Eur. Cytokine Netw., 9, 205-212 (1998)
31 Hla, T. and Neilson, K., Human cyclooxygenase-2 cDNA. Proc. Natl. Acad. Sci. U.S.A., 89, 7384-7388 (1992)   DOI   ScienceOn
32 Stein, B. and Sutherland, M. S. K., IL-6 as a drug discovery target. Drug Discov. Today, 3, 202-213 (1998)   DOI   ScienceOn
33 Nathan, C. and Xie, Q. W., Nitric oxide synthases: roles, tolls and controls. Cell, 78, 915-918 (1994)   DOI   ScienceOn
34 Repetto, M. G. and Llesuy, S. F., Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz. J. Med. Biol. Res., 35, 523–534 (2002)   DOI
35 Henderson, B., Poole, S., and Wilson, M., Bacterial modulins: a novel class of virulence factors which cause host tissue pathology by inducing cytokine synthesis. Microbiol. Rev., 60, 316-341 (1996)
36 Vane, J. R., Bakhle, Y. S., and Botting, R. M.. Cyclooxygenases 1 and 2. Annual Rev. Pharmacol. Toxicol., 38, 97-120 (1998)   DOI   ScienceOn
37 O'Sullivan, M. G., Chilton, F. H., Huggins, E. M. Jr., and McCall C. E., Lipopolysaccharide priming of alveolar macrophages for enhanced synthesis of prostanoids involves induction of a novel prostaglandin H synthase. J. Biological. Chem., 267, 14547-14550 (1992)
38 Hawkey, C. J., COX-2 inhibitors. Lancet, 353, 307-314 (1999)   DOI   ScienceOn
39 Scott, M. G. and Hancock, R. E., Cationic antimicrobial peptides and their imultifunctional role in the immune system. Crit. Rev. Immunol., 20, 407-431 (2000)
40 Henkel, T., Machleidt, T., Alkalay, I., Kronke, M., Ben-Neriah, Y., and Baeuerle, P. A., Rapid proteolysis of I kappa B-alpha is necessary for activation of transcription factor NF-kappa B. Nature, 365, 182-185 (1993)   DOI   PUBMED   ScienceOn
41 Wei, X. C. and Smith, I. G., Altered immune response in mice lacking inducible nitric oxide synthetase. Nature, 375, 408- 411 (1995)   DOI   ScienceOn