한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제22권4호
  • /
  • Pages.336-341
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  • 2005
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개에서 불용성 근위축과 골절 치유에 대한 Nandrolone decanoate의 효과

Effect of Nandrolone Decanoate on Disuse Muscle Atrophy and Bone Beating in Dogs

  • Yun Seong-jin (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Seoul National University) ;
  • Lim Ji-hey (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Seoul National University) ;
  • Rahman Md. Mizanur (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Seoul National University) ;
  • Byeon Ye-eun (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Seoul National University) ;
  • Kim Wan-hee (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Seoul National University) ;
  • Kweon Oh-kyeong (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Seoul National University)
  • 발행 : 2005.12.01
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초록

개에서 Nandrolone decanoate(ND)의 수술 후 외고정 등에 의한 불용성 근위축과 골절 치유에 미치는 영향을 평가하기 위하여 본 실험을 실시하였다 $3.3\~5.5kg,\;2\~4$세의 건강한 잡종견 20두를 대조군(A군, 4두), 1.5mg/kg ND 투여군(수술 직후 투여 - B군, 8두), 7.5mg/kg ND 투여군(수술 직후 투여 - C군, 8두)으로 나누어 무작위로 실험에 사용하였다. 요골의 골간에 골절을 유발하여 1mm 간격을 유지하며 plate로 고정하였다 ND (Deca-Durabolin 50mg/ml, 한화제약 주식회사)투여 군은 1.5mg/kg혹은 7.5mg/kg용량의 ND를 수술 직후, 일주일에 일회씩 근육 내로 8주간 투여하였다. 수술 후 4주간 로버트 존스 포대법을 실시한 후 제거하였다. 체중과 근육의 변화를 수술 직후, 수술 2, 4, 8주 후에 각각 측정하였다 ND 투여 후 4주와 8주에 조직학적 검사를 실시하였다. 근육량의 변화는 A, B 및 C 각 군에서 수술 8주 후에 수술 전 근육량의 $-2.75\pm0.16\%,\;1.68\pm0.11\%$및 $1.74\pm0.48\%$의 변화를 보였다. 근육량은 대조군과 비교하여 투여군에서 투여 4주 후부터 유의적으로 증가하였다 (p<0.05). 투여군에서 용량 의존적 차이는 보이지 않았다. 조직학적 평가에서 ND투여 4주 후 투여군에서 골절된 골 사이에 형성된 섬유 결합조직층이 대조군과 비교하여 더 많이 형성되었다. 5주 후 투여군에서 섬유결합 조직층이 더욱 치밀해졌다. 이상의 결과로 보아 ND투여는 수술 후 외고정 등에 의한 불용성 근위축의 예방과 골절 치유에 유용할 것으로 사료된다.

Anabolic steroid compounds are widely used for the increase of muscle mass, density of bone and athletic ability. The present study conducted to evaluate the effects of nandrolone decanoate (ND), one of the anabolic steroid compounds, on disuse muscle atrophy and healing process of bone in dogs. Twenty physically healthy dogs of both sexes were used in this experiment and divided into three groups: group A (control), group B (low dose M-1.5 mg/kg) and group C (high dose ND-7.5 mg/kg). One-mm strip of full thickness bone was excised from the radius below the pronator teres muscle for the artificial fracture and then the fractured ends were fixed in apposition with bone plate leaving 1mm gap, and finally immobilized externally by Robert John's bandage for 4 weeks. ND was administered intramuscularly once a week far 8 weeks. Body weight, muscle mass change and fracture gap of the bone were evaluated immediately after surgery, and 2, 4, and 8 weeks after surgery. The rates of muscle mass change 8 weeks after surgery were $-2.75\pm0.16\%,\;1.68\pm0.11\%\;and\;1.74\pm0.48\%$ in groups A, B and C, respectively. The significant increase (p<0.05) of muscle mass increments were found in the treated groups. The fibrous connective tissue layer in the fracture gaps of the treated groups increased more than the control, especially in the group C at 4th week. More dense fibrous connective tissue were found in the treated groups at 8th week. Collectively, our results suggested that ND was an effective anabolic agent for the immobilized disuse muscle and bone healing.

키워드

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