각시붕어, Rhodeus uyekii와 칼납자루, Acheilognathus koreensis에 대한 염산리도카인과 Clove Oil의 마취효과

Effect of Lidocaine Hydrochloride and Clove Oil as an Anaesthetic on Korean Rose Bitterling, Rhodeus uyekii and Oily Bifterling, Acheilognathus koreensis

  • 강언종 (국립수산과학원 내수면양식연구소) ;
  • 김은미 (한국해양대학교 해양환경 생명과학부) ;
  • 김영자 (한국해양대학교 해양과학기술연구소) ;
  • 임상구 (국립수산과학원 내수면양식연구소) ;
  • 심두생 (국립수산과학원 내수면양식연구소) ;
  • 김용호 (국립수산과학원 내수면양식연구소) ;
  • 박인석 (한국해양대학교 해양환경 생명과학부)
  • Kang Eon-Jong (Inland Aquaculture Research Institute National Fisheries Research & Development Institute) ;
  • Kim Eun-Mi (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Kim Young Ja (Research Institute of Marine Science and Technology Korea Maritime University) ;
  • Lim Sang Gu (Inland Aquaculture Research Institute National Fisheries Research & Development Institute) ;
  • Sim Doo Saing (Inland Aquaculture Research Institute National Fisheries Research & Development Institute) ;
  • Kim Yong-Ho (Inland Aquaculture Research Institute National Fisheries Research & Development Institute) ;
  • Park In-Seok (Division of Marine Environment and Bioscience, Korea Maritime University)
  • 발행 : 2005.11.01

초록

각시붕어, Rhodeus uyekii (Mori, 1935)와 칼납자루, Acheilognathus koreensis (Kim and Kim, 1990)에 대한 염산리도카인과 Clove oil의 $10^{\circ}C,\;15^{\circ}C,\;20^{\circ}C$$25^{\circ}C$ 수온 조건에서의 마취 효과를 조사하였다. 3분 미만의 완전마취(Stage A5)와 10분 미만의 완전회복(Stage R5)을 기준시, 마취 수온 $20^{\circ}C$에서의 염산리도카인 적정 농도는 각시붕어와 칼납자루에서 각각 250${\~}$550 ppm, 150${\~}$550 ppm 이었으며 Clove oil에서는 각각 40${\~}$200 ppm, 80${\~}$240 ppm 이었다. 본 실험의 2종을 대상으로 한 염산리도카인과 Clove oil에 의한 마취시 negative곡선의 농도 의존성을 보였다. 회복시간은 마취제 농도에 따라 다소 변이를 보였으나, 일반적으로 고농도 마취는 긴 회복시간을 보였다. 마취 수온과 마취 효과는 있어 양성의 상관관계가 있어, 저온에서의 마취시 마취시간과 회복시간이 더 소요되었다. 본 연구에서 사용된 어류 마취제 염산리도카인과 Clove oil은 본 실험에 사용된 종들에 효과적인 마취제임을 시사한다. 본 연구로부터의 결과는 각시붕어와 칼납자루의 사육시 어체 진정이 요구되는 취급이나, 이들의 양식시 유용할 것이다.

The efficacy of lidocaine hydrochloride and Clove oil as anaesthetics was evaluated in the Korean rose bitterling, Rhodeus uyekii (Mori, 1935) and oily bitterling, Acheilognathus koreensis (Kim and Kim, 1990) at four different temperatures of $10^{\circ}C,\;15^{\circ}C,\;20^{\circ}C$ and $25^{\circ}C$. When complete anaesthesia was acquired less than 3 min and recovery was acquired less than 10 min, the optimal dose range of lidocain hydrochloride at $20^{\circ}C$ was 250${\~}$550 ppm in Korean rose bitterling, and 150${\~}$550 ppm in oily bitterling, respectively. In case of Clove oil, the optimal dose range at $20^{\circ}C$ was 40${\~}$200 ppm in Korean rose bitterling and 80${\~}$240 ppm in oily bitterling, respectively. Both of lidocaine hydrochloride and Clove oil resulted in a negatively dose-dependent manner for anaesthesia induction time in these two species. Recovery times were more variable in relation to anaesthetic doses, but in general higher anaesthetic doses resulted in similar or longer recovery time. As expected, the lower temperature resulted in longer anaesthesia induction and recovery time. The study demonstrated that lidocaine hydrochloride and Clove oil can be used as effective anaesthetics in these two species. The results from this study could be useful for aquaculturists industry and other related husbandry practices that require anaesthesia of Korean rose bitterling and oily bitterling.

키워드

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