• Korean Journal of Ichthyology
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• v.19 no.4
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• pp.286-291
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• 2007

The bitterling, Acheilognathus koreensis spermatozoon has been examined by electron microscopy. The epididymal spermatozoa of A. koreensis are representing typical characteristic of cyprinid spermatozoa including the lateral insertion of flagellum, the organization of centriolar complex in shallow nuclear fossa and the asymmetrical arrangement of mitochondria. The sperm mid-piece contains a large mitochondrion characteristic enclosed by membranous vesicles. The mitochondria aspect is different from that of other cyprinid spermatozoa, which their mitochondria have a conventional aspect and never fuse to form a mitochondrial derivative. In term of sperm evolution, the fused mitochondria are regarded as the apomorphic character in comparison with the separate mitochondria. The single mitochondrion is not found in cyprinid spermatozoon except for Rodeus and Pungtungia.

• Korean Journal of Ichthyology
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• v.2 no.1
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• pp.47-52
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• 1990

Anew species of cyprinid fish Acheilognathus koreensis is described from specimens collected in several rivers of southern part of Korea. In general aspect it is similar to A. liηzbata but differs form this by having large snout length, narrow interorbital width and characters of early developmental stage. The new species is distinguished from A. signifer by color parttern of dorsal and anal fin ray in male, and egg form and ovipositor length in female.

• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.159-166
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• 2004

The effects of polychlorinated biphenyls (PCBs) on the reproduction of the oily bitterling, Acheilognathus koreensis were investigated using histological methods. The experimental fishes were exposed to 0. 3.4, 9.3 and 25.8 ${\mu}g\; L^{-1}$ PCBs fey 8 weeks. Dead fishes and abnormal behavior were not observed in control and PCBs exposure group. In compared with control group, PCBs exposure fishes showed differences of gonadal development, gonadosomatic index (GSI), hepatosomatic index (BSI), nuptial color index (NCI) and ovipositor length index (OLI). These results showed that PCBs could disrupt reproductive process in the fish.

• Animal Systematics, Evolution and Diversity
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• v.7 no.2
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• pp.189-194
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• 1991

A new speices of cyprinid fish, Acheilognathus somjinensis is described based on the specimens collected in the Somjin River, Sinpyong-myon, Imsil -gun , Chollabuk-do province of Korea. In general morphology it is similar to A. koreensis. however, the new speices differs from the latter in having longer ovipositor of female in spawing season and showing pear-shaped eggs. In hybridization experiments, it was found that A.somjinensis is repoductively isolated from A.koreensis.

• Korean Journal of Ichthyology
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• v.23 no.2
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• pp.150-157
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• 2011

In this study we described reproduction study of Korean endemic species, Acheilognathus koreensis and three other acheilognathinae species A. signifer, Rhodeus pseudosericeus, and R. uyekii. Morphology of egg shape and larvae just hatched is unique by the species respectively. There is no interrelation factor between size of female and number of spawned eggs per one time. Minium water temperature for spawning of A. koreensis was observed in $13.0^{\circ}C$ having low hatching rate with 43.5 percent. The egg of A. koreensis began to hatch approximately 79 hours after insemination. As higher as water temperature until $22.0^{\circ}C$ hatching elapsed time for developing eggs of A. koreensis and three species in this study was shortened. A. koreensis was grew up to be a adult enough which can be join to new production for three hundred days after hatching with 52.4 mm in total length. This reproduction study will be applied to preservation of Korean endemic fresh water fishes basically.

• Korean Journal of Ichthyology
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• v.18 no.2
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• pp.87-96
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• 2006

Reproduction and organ system of the oily bitterling, Acheilognathus koreensis in Isa stream were investigated by means of histological methods. Sex ratio was observed 1 : 1 in female to male. The gonadosomatic index (GSI) of the female showed two peak in December and April. The ovipositor length index (OLI) represented irregular change. From the histological analysis of the organ system, epidermal atrophy and hyperplasia of pigment cell were observed in the skin. Epithelial layer lifting of the lamella and bifurcation of the filament were observed in the gill. Also histological changes as congestion, cytoplasmic degeneration of hepatic cell, glomerular dilatation, hyaline droplet degeneration of renal tubule epithelium and pycnosis of interstitial cell were identified in the liver and kidney, respectively.

• Animal cells and systems
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• v.16 no.4
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• pp.321-328
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• 2012

The oily bittering Acheilognathus koreensis is a freshwater species that is endemic to Korea and is experiencing severe declines in natural populations as a result of habitat fragmentation and water pollution. For the conservation and restoration of this species, it is necessary to assess its genetic diversity at the population level. We developed 13 polymorphic microsatellite loci that were used to analyze the genetic diversity of two populations collected from the Kum River and the Tamjin River in Korea. All loci exhibited Mendelian inheritance patterns when examined in controlled crosses. Both populations revealed high levels of variability, with the number of alleles ranging from 3 to 20 and observed and expected heterozygosities ranging from 0.500 to 0.969 and from 0.529 to 0.938, respectively. None of the loci showed significant deviation from Hardy-Weinberg equilibrium, and one pair of loci showed significant linkage disequilibrium after Bonferroni correction. Pairwise $F_{ST}$ and genetic distance estimation showed significant differences between two populations. These results suggest that the microsatellites developed herein can be used to study the genetic diversity, population structure and conservation measure of A. koreensis.

• Journal of Aquaculture
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• v.18 no.4
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• pp.272-279
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• 2005

The efficacy of lidocaine hydrochloride and Clove oil as anaesthetics was evaluated in the Korean rose bitterling, Rhodeus uyekii (Mori, 1935) and oily bitterling, Acheilognathus koreensis (Kim and Kim, 1990) at four different temperatures of $10^{\circ}C,\;15^{\circ}C,\;20^{\circ}C$ and $25^{\circ}C$. When complete anaesthesia was acquired less than 3 min and recovery was acquired less than 10 min, the optimal dose range of lidocain hydrochloride at $20^{\circ}C$ was 250${\~}$550 ppm in Korean rose bitterling, and 150${\~}$550 ppm in oily bitterling, respectively. In case of Clove oil, the optimal dose range at $20^{\circ}C$ was 40${\~}$200 ppm in Korean rose bitterling and 80${\~}$240 ppm in oily bitterling, respectively. Both of lidocaine hydrochloride and Clove oil resulted in a negatively dose-dependent manner for anaesthesia induction time in these two species. Recovery times were more variable in relation to anaesthetic doses, but in general higher anaesthetic doses resulted in similar or longer recovery time. As expected, the lower temperature resulted in longer anaesthesia induction and recovery time. The study demonstrated that lidocaine hydrochloride and Clove oil can be used as effective anaesthetics in these two species. The results from this study could be useful for aquaculturists industry and other related husbandry practices that require anaesthesia of Korean rose bitterling and oily bitterling.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2004.12a
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• pp.86-86
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• 2004

• Korean Journal of Ichthyology
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• v.18 no.3
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• pp.170-177
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• 2006

Morphology and distribution of the minute tubercles projected on the skin surface of larvae with its development was observed in the Korean bitterling, Acheilognathus koreensis, known as an endemic freshwater fish. The epidermis of the larvae consisted of a thin single layer, having smaller basophilic flat or round-flattened basal cells. In between the single cell layer, two or three layers were added and they consisted mainly of large epidermal cells just above basal cells. These large unicellular epidermal cells were mainly scale-shaped and rarely cone-shaped, and do not give any histochemical tests for mucosubstances. They were present in anterior region and most region of yolk sac. Whereas, vestigial epidermal cells were distributed in the body region and the caudal fin-fold region. These two kinds of epidermal cells, called minute tubercles, increased in number and height from Just to 8 days after hatching, but as the larvae develop gradually, they became to reduce. At 31days after hatching of free swimming stage and absolute absorption stage of the yolk sac, the minute tubercles did not exist on the whole skin of the larvae.