Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Protective Effect of Electrolyzed Reduced Water on the Paraquat-induced Oxidative Damage of Human Lymphocyte DNA

Paraquat에 의한 사람 임파구 DNA 손상에 대한 환원전리수의 보호효과

  • Park, Eun-Ju (Department of Food and Nutritional Sciences, Kyungnam University) ;
  • Ryoo, Kun-Kul (Division of Material & Chemical Engineering, Soonchunhyang University) ;
  • Lee, Yoon-Bae (Division of Material & Chemical Engineering, Soonchunhyang University) ;
  • Lee, Jong-Kwon (Division of Material & Chemical Engineering, Soonchunhyang University) ;
  • Lee, Mi-Young (Division of Life Science, Soonchunhyang University)
  • 박은주 (경남대학교 식품영양학과) ;
  • 류근걸 (순천향대학교 신소재화학공학부) ;
  • 이윤배 (순천향대학교 신소재화학공학부) ;
  • 이종권 (순천향대학교 신소재화학공학부) ;
  • 이미영 (순천향대학교 생명과학부)
  • Published : 2005.06.30
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Abstract

Electrolyzed reduced water (ERW), showing extremely negative oxidation-reduction potential, was used to investigate the effects of paraquat-induced damages on DNA from human lymphocyte. The effect of ERW on paraquat-induced oxidative DNA damage in human lymphocytes was evaluated by Comet assay (single-cell gel electrophoresis) quantified as percentage fluorescence in tail. Comet assay has been used widely to assess the level of the DNA damage in individual cells. Lymphocytes were oxidatively challenged with various concentrations of paraquat for 30 min at $37^{\circ}C$, and were then treated with electrolyzed reduced water for 30 min. The oxidative DNA damage by paraquat, as indicated by the fluorescent tail in DNA, increased in a dose-dependent manner. However, oxidative damage of the DNA was almost completely prevented upon treatment with electrolyzed reduced water.

본 연구에서는 매우 낮은 음의 산화환원전위를 나타내는 환원전리수가 paraquat에 의한 사람 임파구 DNA의 손상에 미치는 영향을 Comet assay를 사용하여 조사하였다. 환원전리수 처리에 의해서 paraquat에 의해 산화적으로 손상된 DNA가 회복되는 정도를 손상된 DNA로 인한 꼬리부분의 형광광도의 %비율로 나타내었다. Comet assay는 개별 세포의 DNA의 산화적 손상을 측정하는데 널리 사용되고 있다. 사람 임파구에 다양한 농도의 paraquat을 $37^{\circ}C$에서 30분간 처리한 후, 환원전리수를 첨가하여 30분간 반응시켰다. 그 결과 paraquat에 의한 임파구 DNA의 손상은 paraquart 농도증가에 의존적으로 증가하였다. 그러나 환원전리수를 처리한 결과 DNA의 산화적 손상이 paraquat 미처리 대조군 수준으로 거의 다 복구되었다.

Keywords

References

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