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Protective Effect of Electrolyzed Reduced Water on the Paraquat-induced Oxidative Damage of Human Lymphocyte DNA  

Park, Eun-Ju (Department of Food and Nutritional Sciences, Kyungnam University)
Ryoo, Kun-Kul (Division of Material & Chemical Engineering, Soonchunhyang University)
Lee, Yoon-Bae (Division of Material & Chemical Engineering, Soonchunhyang University)
Lee, Jong-Kwon (Division of Material & Chemical Engineering, Soonchunhyang University)
Lee, Mi-Young (Division of Life Science, Soonchunhyang University)
Publication Information
Applied Biological Chemistry / v.48, no.2, 2005 , pp. 155-160 More about this Journal
Abstract
Electrolyzed reduced water (ERW), showing extremely negative oxidation-reduction potential, was used to investigate the effects of paraquat-induced damages on DNA from human lymphocyte. The effect of ERW on paraquat-induced oxidative DNA damage in human lymphocytes was evaluated by Comet assay (single-cell gel electrophoresis) quantified as percentage fluorescence in tail. Comet assay has been used widely to assess the level of the DNA damage in individual cells. Lymphocytes were oxidatively challenged with various concentrations of paraquat for 30 min at $37^{\circ}C$, and were then treated with electrolyzed reduced water for 30 min. The oxidative DNA damage by paraquat, as indicated by the fluorescent tail in DNA, increased in a dose-dependent manner. However, oxidative damage of the DNA was almost completely prevented upon treatment with electrolyzed reduced water.
Keywords
electrolyzed reduced water; paraquat; human lymphocyte DNA;
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