Repellent and Pesticidal Effect of Ginkgo biloba Leaves Extracts on the Tetranichus urticae, Aphis gossypii and Myzus persicae

점박이응애(Tetranichus urticae), 목화진딧물(Aphis gossypii)과 복숭아혹진딧물(Myzus persicae)에 대한 은행잎 추출물의 살충 및 기피효과

  • Lee, In-Hwa (Department of Environmental Engineering, College of EngineeringScience, Chosun University) ;
  • Seol, Myung-Su (Department of Environmental Engineering, College of EngineeringScience, Chosun University) ;
  • Park, Jong-Dae (Jeollanam-do Agricultural Research and Extension Services)
  • Published : 2005.06.30

Abstract

To overcome the problems associated with chemical pesticides, biological pest controls agent extracted from Ginkgo biloba was studied. Insecticidal activities components in Ginkgo biloba extracts were analyzed using high performance liquid chromatography (HPLC). This results of HPLC analysis, GG-W80 were included bilobalide $611\;{\mu}g/kg$, ginkgolide A $37\;{\mu}g/kg$ and ginkgolide B $243\;{\mu}g/kg$, while YG-W80 were included bilobalide $214\;{\mu}g/kg$ and ginkgolide B $46\;{\mu}g/kg$. The biological activity of Ginkgo biloba extracts were conducted to repellent and pesticidial effect of Tetranichus urticae, Aphis gossypii and Myzus persicae treated with Ginkgo biloba leaves extracts. Mortalities of adult T. urticae to green Ginkgo biloba extracts (GG-W80) and yellow Ginkgo biloba extracts (YG-W80) were shown 98.3% and 20.0%, respectively. From these results, terpenes components in Ginkgo biloba extracts could be use for biological controls for T. urticae.

천연식물자원으로부터 생물농약을 개발하기 위하여 점박이응애(Tetranichus urticae), 목화진딧물(Aphis gossypii)과 복숭아혹진딧물(Myzus persicae)에 대해 푸른은행잎 추출물(GG-W80)과 노란은행잎 추출물(YG-W80)의 살충효과와 기피효과를 leaf disk법과 유묘검정법에 의해 조사하였다. 점박이응애에 대한 살충효과는 GG-W80이 98.3%로 YG-W80의 20.0%보다 우수하였다. 점박이응애에 강한 살충효과를 보이는 생리활성물질은 high performance liquid chromatography(HPLC)를 이용해 동정하였다. 푸른은행잎 추출물에서는 각각 bilobalide $611\;{\mu}g/kg$, ginkgolide A $37\;{\mu}g/kg$와 ginkgolide B $243\;{\mu}g/kg$이 동정되었으며, 노란은행잎 추출물에서는 bilobalide $214\;{\mu}g/kg$와 ginkgolide B $46\;{\mu}g/kg$이 동정되었으나 ginkgolide A는 확인되지 않았다. 이상의 결과는 은행잎에 존재하는 terpene계 화합물인 bilobalide와 ginkgolide A, B가 점박이응애에 살충력을 갖고 있음을 알수 있으며, 이들 성분을 포함한 은행잎 추출물이 점박이응애에 대한 생물학적 방제 가능성을 가지고 있음을 시사하였다.

Keywords

References

  1. Nam, S. J., Kim K. U., Shin. D. H. and Hwang, S. J. (1997) Identification of biologically active substances from Ginkgo biloba L. Kor. J. Weed Sci. 17, 421-430
  2. Kang, S. S., Koh, Y. M., Kim, J. S. and Lee, M. W. (1995) Phytochemical analysis of Ginkgo biloba yellow leaves. Kor. J. Pharmacogn. 26, 23-26
  3. Fukuda, T. (1932) The pharmacological effects of flavone groups. Arch. Exptl. Path. Pharmacol. 164, 685-696 https://doi.org/10.1007/BF01860264
  4. Jeney, A. V. and Czimmer, A. (1936) The action of quercetin and quercitrin on the ununjured and poisoned frog heart. Arch. Exptl. Path. Pharmacol. 183, 571-579 https://doi.org/10.1007/BF01867514
  5. Lang, Q., and Wai, C. M. (1999) An extraction method for determination of ginkgolides and bilobalide in ginkgo leaf extracts. Anal. Chem. 71, 2929-2933 https://doi.org/10.1021/ac981251h
  6. Lee, K. S. (1997) Evaluation on the effects of pesticide residues to agroecosystem in Korea. Kor. J. Environ. Agri. 16, 80-93
  7. Sawacki, R. M. (1979) Resistance of insects to insecticides. Span. 22, 51-55
  8. Lee, S. R. (1982) Overall assessment of organochlorine insecticide residues in Korean foods. Kor. J. Food Sci. Technol. 14, 82-94
  9. Koshihara, T. (1988) Chemical control of the diamondback moth in Japan. Jap. Pesti. Information. 53, 14-17
  10. Yang, H. S., Lee, D. H. and Lee, S. C. (1988) In New agricultural chemicals, Hyangmunsa, Seoul, Korea
  11. Song, S. S. (1991) Resistance of Diamondback moth (Plutella xylostella L. : Yponomeutidae : Lepidoptera) against Bacillus thuringiensis Berliner. Korean J. Appl. Entomol. 30, 291-293
  12. Rhim, S. L. (1994) Development and trend of microbial pesticide using Bacillus thuringiensis, Kor. J. Biotechnol. Bioeng. Bioeng. News 1, 24-35
  13. Lee, S. Y., Yoo, J. S., Moon, S. J., Le, S. G. and Kim, C. S. (2003) Fumigant and repellency effects of terpence against the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae). Kor. J. Appl. Entomol. 42, 249-255
  14. Jang, C., Hwang, I. C., Yu, Y. M. and Choe, K. R. (1998) Action properties and insecticidal effects of thiamethoxam to the melon aphid, Aphis gossypii, and diamondback moth, plutella xylostella, Kor. J. Pesticide Sci. 2, 126-136
  15. Kim, G. H. and Lee, Y. S. (1999) Selective toxictiy of aphicides to the predator Harmonia axyridis (Coleoptera: Coccinellidae) and Myzus persicae (Homoptera: Aphididae). Kor. J. Pesti. Sci. 3, 84-89
  16. Matsumoto, T. and Sei, T. (1987) Antifeedant activities of Ginkgo biloba L. components against the larva of pieris rapae crucivora. Agric. Biol. Chem. 51, 249-250
  17. Ahn, Y. J., Kwon, M., Park, H. M. and Han, C. K. (1997) Potent insecticidal activity of Ginkgo biloba derived trilactone terpenes against Nilaparvata lugens. In Phytochemicals for Pest Control. ACS Symp. Ser. No. 658, Am. Chem. Soc., Washington, DC, pp. 90-105