Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation of Mutants Susceptible to Rice Blast from DEB-treated Rice Population

DEB 처리에 의해 유도된 벼 돌연변이 집단으로부터 도열병 감수성 돌연변이 분리

  • Kim, Hye-Kyung (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Sang-Kyu (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Han, Mu-Ho (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Jeon, Yong-Hee (National Institute of Crop Sciences, Rural Development Administration) ;
  • Lee, Gi-Hwan (Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Youn-Hyung (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Bhoo, Seong-Hee (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Hahn, Tae-Ryong (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Jeon, Jong-Seong (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • 김혜경 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이상규 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 한무호 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 전용희 (농촌진흥청 작물과학원) ;
  • 이기환 (농촌진흥청 작물과학원 영남농업연구소) ;
  • 이윤형 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 부성희 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 한태룡 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 전종성 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2005.12.31
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Abstract

Rice blast, which is caused by the fungus Magnaporthe grisea, is one of the most destructive diseases of rice. To identify genes involving in the signal transduction pathways that mediate rice blast resistance, we screened over 2,000 mutant lines of a highly resistant variety RIL260 that were generated by using a DEB (1, 3-Butadiene diepoxide) treatment method. In the mutant population, the frequency of albino plants was 6.7%, indicating that this population has a high frequency of mutations in the genome. The primary screening identified 29 mutant plants that exhibit a complete or partial loss of the resistance to rice blast. Among them, M5465, the most susceptible line, was subsequently examined by DNA gel-blot experiments using DNA molecular markers of Pi5(t) that has been previously identified as a durable resistance locus in RIL260. The result revealed that a large deletion and rearrangement of genomic DNA occurred in the Pi5(t) locus. The results suggest that DEB can be used as an efficient mutagen to induce large scale mutations in the rice genome. The isolated mutants should be useful for elucidating the Pi5(t)-mediated signaling pathways of rice blast resistance.

도열병은 곰팡이 균(Magnaporthe grisea)에 의해 발병되는 것으로, 벼 수확량에 가장 큰 손실을 일으킨다. 본 연구에서는 벼 도열병 저항성 신호전달 체계에 관여하는 유전자를 분리하기 위하여 DEB(1, 3-Butadiene diepoxide) 처리를 통하여 벼 도열병 저항성 품종인 RIl260의 돌연변이 2,000여 종을 생산하고, 이들로부터 병 저항성 변이 개체를 조사하였다. 돌연변이 집단에서 백변종 돌연변이의 비율은 6.7%로 매우 높았으며, 이것은 DEB 처리에 의해서 생산된 집단 내에 돌연변이가 높은 빈도로 발생하였음을 보여준다. 돌연변이 집단의 병 저항성 분석을 통하여 완전히 혹은 부분적으로 벼 도열병에 저항성을 상실한 29개의 돌연변이체를 분리하였다. 이들 중에서 가장 심한 이병성 라인으로 확인된 M5465는 DNA 혼성화 반응 분석을 사용하여 분석하였으며, RIL260 품종에서 도열병 병 저항성과 밀접한 관련을 갖고 있는 것으로 보고된 Pi5(t) 유전좌위의 DNA 표지들이 실험에 사용되었다. 이 결과들은 M5465에서 Pi5(t) 유전좌위 내부에 DNA의 큰 결손 및 재배열이 있었음을 보여준다. 분리된 병 저항성 돌연변이 라인들은 Pi5(t)에 의해 매개된 도열병 저항성의 신호 전달 과정을 이해하는 데 유용하게 사용 될 수 있을 것이다.

Keywords

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