Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Qualitative PCR Detection of GM rices (Milyang 204 and Iksan 483) developed in Korea

국내에서 개발된 GM 쌀 (밀양 204호, 익산 483호)에 대한 정성 PCR 분석법 개발

  • Kim, Jae-Hwan (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Song, Hee-Sung (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Jee, Sang-Mi (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Ryu, Tae-Hun (Division of Biosafety, National Institute of Agricultural Biotechnology) ;
  • Kim, Dong-Hern (Division of Biosafety, National Institute of Agricultural Biotechnology) ;
  • Kim, Hae-Yeong (Department of Food Science and Biotechnology, Kyung Hee University)
  • 김재환 (경희대학교 생명공학원) ;
  • 송희성 (경희대학교 생명공학원) ;
  • 지상미 (경희대학교 생명공학원) ;
  • 류태훈 (농촌진흥청 농업생명공학연구원 생물안전성과) ;
  • 김동헌 (농촌진흥청 농업생명공학연구원 생물안전성과) ;
  • 김해영 (경희대학교 생명공학원)
  • Published : 2005.12.31
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Abstract

For the development of qualitative PCR detection method of genetically modified rice (Oryza sativa L.), rice species specific gene, SAMDC1 (S-adenosylmethionine decarboxylase), was selected and validated as suitable for use as an endogenous reference gene in rice. The primer pair OsSAMDC1-5'/3' with 110 bp amplicon was used for amplification of the rice endogenous gene, SAMDC1 and no amplified product was observed from 19 different plants as templates. Qualitative PCR method was assayed with 2 different GM rices (Milyang 204 and Iksan 483) developed in Korea. For the qualitative PCRs, the construct-specific detection primer pairs were constructed. Os204-5'/OsNOS-3' amplifying the junction region of GUS gene and NOS terminator introduced in Milyang 204 gave rise to an amplicon 172 bp; also, Os483-5'/OsNOS-3' amplifying the junction region of Bar gene and NOS terminator introduced in Iksan 483 gave rise to an amplicon 161 bp.

국내에서 개발된 GM 쌀인 밀양 204호, 익산 483호의 정성 PCR 분석법의 개발을 위해 쌀의 내재 유전자로써 SAMDC1(S-adenosylmethionine decarboxylase)에 특이적인 primer (OsSAMDC1-5'/3')쌍을 제작하여 쌀을 포함한 20개 작물에 대해 PCR을 수행하여 쌀에 특이적으로 증폭되는 것을 확인하였다. 또한, 밀양 204호에 삽입된 GUS 유전자와 NOS terminator 연결 부위를 증폭시켜 172 bp의 PCR 산물을 얻을 수 있는 primer(Os204-5'/OsNOS-3')와 익산 483호에 삽입된 bar 유전자와 NOS terminator 연결 부위를 증폭시켜 161 bp의 PCR 산물을 얻을 수 있는 primer(Os483-5'/OsNOS-3')을 이용하여 국내 개발된 GM 쌀인 밀양 204호, 익산 483호의 PCR 정성 분석법을 확립하였다.

Keywords

References

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