Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Purification of Oxytocin-related Peptide, Isotocin from the Brain of Conger Eel Conger myriaster

붕장어(Conger myriaster)의 뇌로부터 Oxytocin-related Peptide, Isotocin의 정제

  • GO Hye-Jin (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM Chan-Hee (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM Eun Jung (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM In Hae (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • AN Sang Hyun (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • SOHN Hee-Young (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • PARK Jin-IL (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • PARK HEE Yun (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • YOON Ho Dong (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • PARK Nam Gyu (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
  • 고혜진 (부경대학교 수산과학대학 생물공학과) ;
  • 김찬희 (부경대학교 수산과학대학 생물공학과) ;
  • 김은정 (부경대학교 수산과학대학 생물공학과) ;
  • 김인혜 (부경대학교 수산과학대학 생물공학과) ;
  • 안상현 (부경대학교 수산과학대학 생물공학과) ;
  • 손희영 (부경대학교 수산과학대학 생물공학과) ;
  • 박진일 (국립수산과학원 생명공학연구단) ;
  • 박희연 (국립수산과학원 생명공학연구단) ;
  • 윤호동 (국립수산과학원 생명공학연구단) ;
  • 박남규 (부경대학교 수산과학대학 생물공학과)
  • Published : 2005.10.01
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Abstract

Oxytocin (OT)-related peptide, isotocin was purified from the brain extract of conger eel (Conger myriaster) using reverse-phase, ion-exchange and size exclusion high performance liquid chromatography (HPLC). The sequence of the peptide, with a molecular weight of 967.30 Da, was determined as Cys-Tyr-Ile-Ser-Asn­Cys-Pro-Ile-Gly-$NH_2$, where the Cys between 1st and 6th residues made an intramolecular disulfide bridge by the automated amino acid sequence analysis and MALDI-TOF mass spectrometry. The sequence was confirmed by identical elution with the purified and synthetic peptide using the HPLC system. As a result of homology investigation, the primary structure of this peptide was the same as that of OT -superfamily member, isotocin. The synthetic peptide showed a contractile activity at a minimal effective concentration of $10^{-7}M$ on the intestinal smooth muscle of goldfish (Carassius auratus).

Keywords

References

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