한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제18권2호
  • /
  • Pages.240-245
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  • 2005
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지

더덕에서 Glutathione S-transferase (ClGST) 유전자의 분리

Isolation of Gglutatihone S-Ttransferase(ClGST) Gene from Codonopsis lanceolata

  • 김진주 (경희대학교 생명과학대학 한방재료가공학과) ;
  • 양덕춘 (경희대학교 생명과학대학 한방재료가공학과)
  • Kim Jin-Ju (Dept. of Oriental Medicinal Materal and Processing, College of Life Science Kyung Hee University) ;
  • Yang Deok-Chun (Dept. of Oriental Medicinal Materal and Processing, College of Life Science Kyung Hee University)
  • 발행 : 2005.06.01
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초록

더덕 뿌리에서 유래한 EST cDNA library로부터 GST(glutatione S-transferase)유전자와 높은 상동성을 나타내는 full clone cDNA를 얻었다. 더덕의 GST(glutatione S-transferase), CIGST은 761 bp의 cDNA로 173개의 아미노산을 코딩하는 522 bp의 ORF를 가지고 있으며, A. thaliana(AAC63629) $71\%$, C. chinense(CAI51314) $73\%$, E. esula(AAE65767) $75\%$, H. muticus(CAA55039) $70\%$, N. plumbaginifolia(CAA96431) $77\%$, S. commersonii(AAB65163) $76\%$등 다른 식에서 밝혀져 있는 GST(glutatione S-transferase)와 유의한 상동성을 나타내였다.

A cDNA clone homologous to glutathione S-transferase gene was isolated and characterized from Codonopsis lanceolata(ClGST). The ClGST is 761 nucleotides long and has an open reading frame of 522 bp with a deduced amino acid sequence of 173 residues. The ClGST shows meaning homology to A. thaliana(AAC63629) $71\%$, C. chinense(CAI51314) $73\%$, E. esula(AAE65767) $75\%$, H. muticus(CAA55039) $70\%$, N. plumbaginifolia(CAA96431) $77\%$, S. commersonii(AAB65163).

키워드

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