Antioxidant Activity of Aqueous Extract of Coscinium fenestratum in STZ-Nicotinamide Induced Diabetic Rats

  • Punitha, I.S.R. (Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences) ;
  • Bhat, Nalini (Department of Biochemistry, Kasturba Medical College) ;
  • Rajendran, K. (Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences) ;
  • Shirwaikar, Arun (Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences) ;
  • Shirwaikar, Annie (Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences)
  • Published : 2005.09.01

Abstract

The aqueous extract of Coscinium fenestratum was studied for its antioxidant status in STZ-nicotinamide induced type 2 diabetic rats at two dose levels of 250 mg/kg and 500 mg/kg. At the end of the experimental period, diabetic rats treated with aqueous extract at both dose levels showed a significant increase in the levels of enzymatic antioxidants such as glutathione peroxidase, glutathione synthetase, peroxidase, superoxide dismutase and catalase as compared to the untreated control. Similarly, a significant increase was also observed in the levels of the non enzymatic antioxidants ceruloplasmin, ascorbic acid and tocopherol. The results suggest that the aqueous stem extract of C. fenestratum prevents type 2 diabetes mellitus induced oxidative stress.

Keywords

References

  1. Aebi, E.H., Catalase In: Bergmeyer Methods of Enzymatic analysis, Vol. III, 3rd Edition, Verlag Chemie, Deerfield Beach, Florida, p.273 (1983)
  2. Atanasiu, R.L., Stea, D., and Mateescu, M.A. Direct evidence of ceruloplasmin antioxidant properties. Mol. Cell. Biochem. 189, 127-129 (1998) https://doi.org/10.1023/A:1006945713860
  3. Bruce, A., Freeman, D., and James, C. Biology of disease-free radicals and tissue injury. Lab. Invest. 47, 412-426 (1982)
  4. Chatterjee, I.B. and Nandi, A., Ascorbic acid: a scavenger of oxy radicals. Ind. J. Biochem. Biophys. 28, 233-236 (1991)
  5. Coscinium fenestratum. In: The Wealth of India, Publication, Information and Directorate, CSIR, India, Vol. 2, p. 360 (1950)
  6. Datta, S.C., Mathur R.K., and Baruah, J.N., Minor alkaloids of Coscinium fenestratum root. Indian Drugs 25, 8, 350 (1988)
  7. Desai, L.D., Vitamin E analysis methods for animal tissues. Methods in Enzymol. 105, 138-142 (1984) https://doi.org/10.1016/S0076-6879(84)05019-9
  8. Ewis, S.A. and Abdel Rahman, M.S., Effect of metformin on glutathione and magnesium in normal and TSZ induced diabetic rats. J. Appl. Toxicol. 15, 387-390 (1995) https://doi.org/10.1002/jat.2550150508
  9. Halliwell, B. and Gutteridge, J.M.C., Oxygen toxicity: oxygen radical, transition metal and disease. Biochem. J. 219, 1-14 (1984) https://doi.org/10.1042/bj2190001
  10. Hallliwell, B. and Gutteridge, J.M.C., Free radicals in Biology and Medicine, 2nd edition, Clarendon Press Publications, Oxford, pp. 1-27 (1989)
  11. Harborne, J.B., In: Phytochemical methods, Chapmann & Hall, London, pp.60-66 (1988)
  12. Inefers, H. and Sies, H., The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E. Eur. J. Biochem. 174, 353-357 (1988) https://doi.org/10.1111/j.1432-1033.1988.tb14105.x
  13. Johann, P. and Reinhild, B., Peroxidase, In: Bergmeyer Methods of Enzymatic analysis, Vol. III 3rd Edition, Verlag Chemie, Deerfield Beach, Florida, p. 286 (1983)
  14. Kakkar, P., Dos, B. and Viswanathan, P.N. A., Modified spectrophotometric assay of superoxide dismutase. Ind J. Biochem. Biophys. 21, 130-132 (1984)
  15. Kalavincela, T., In: Studies on rural medicine of Kanyakumari diatrict with special reference to medicinal plants. M. Phil Thesis, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India, 185 (1998)
  16. Kokate, C.K., Purohit, A.P., and Gokhale, S.B., In: Practical Pharmacognosy, Vallabh Prakashan, New Delhi, pp. 107-113 (1994)
  17. Loven, D., Sched, H., and Wilson, H., Effect of insulin and oral glutathione on glutathione levels and superoxide dismutase activities in organs of rats with STZ induced. Diabetes. 35, 503-507 (1986) https://doi.org/10.2337/diabetes.35.5.503
  18. Lowry, O.H., Roesborough, M.J., and Farr, AL., Protein measurement with Folin- Phenol reagent. J. Biol. Chem. 193, 265-275 (1951)
  19. Mahapatra, B., Hypoglycemic activity of Coscinium fenestratum. J. Res. Ayu. Siddha, 18, 89-91 (1997)
  20. Masiello, P., Broca, C; Gross, R., Roye, M., Manteghetti M, Hillaire-Buys, D., Novelli, M., and Ribes, G., Development of a new model of type 2 diabetes in adult rats administered with Streptozotocin and nicotinamide. Diabetes. 47, 224-228 (1998) https://doi.org/10.2337/diabetes.47.2.224
  21. Omaye, S.T., Tumball, J.D., and Sauberlich, H.E., Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. In: McCormick DB, Wright CD (Eds.), Methods in Enzymology, Vol.62, Academic press, New York, pp.1-11 (1979)
  22. Ravin, H.A, An improved colorimetric assay of ceruloplasmin. J. Labor. Clin. Med. 589, 161-168 (1961)
  23. Rotruck, J.T., Pope, A.L., and Ganther, H.E., Selenium biochemical role as a component of glutathione peroxide purification and assay. Science 179, 588-590 (1973) https://doi.org/10.1126/science.179.4073.588
  24. Saxena, A.K., Srivastava, P., and Kale, R.K., Impaired antioxidant status in diabetic rat liver: effect of vandate. Biochem. Pharmacol. 45, 539-542 (1993) https://doi.org/10.1016/0006-2952(93)90124-F
  25. Searle, A.J. and Wilson, R., Glutathione peroxide effect of superoxide, hydroxyl and bromine free radicals on enzyme activity. Int. J. Radiat. Biol. 37, 213-217 (1980) https://doi.org/10.1080/09553008014550261
  26. Selvam, R. and Anuradha, C.V., Effect of oral methionine on blood lipid peroxidation and antioxidants in alloxan induced diabetic rats. J. Nutr. Biochem. 1, 653-658 (1990) https://doi.org/10.1016/0955-2863(90)90027-I
  27. Shirwaikar, A., Rajendran, K., and Punitha, I.S.R., Antidiabetic activity of alcoholic stem extract of C. fenestratum in Streptozotocin nicotinamide induced type 2 diabetic rats. J. Ethnopharmacol. 97, 369-374 (2005) https://doi.org/10.1016/j.jep.2004.11.034
  28. Singh, G.B., Singh, S., Bani, S., and Malhotra, S., Hypotensive action of a Coscinium fenestratum stem extract. J. Ethnopharmacol. 38, 151-155 (1990)
  29. Sinha, K.A., Colorimetric assay of catalase. Anal Biochem. 47, 389-384 (1972) https://doi.org/10.1016/0003-2697(72)90132-7
  30. Stanely Mainzen Prince, P., and Kamalakannan, N., Hypoglycaemic effect of water extracts of Aegle marmelose fruits in Streptozotocin diabetic rats. J. Ethnopharmacol. 87, 207-210 (2003) https://doi.org/10.1016/S0378-8741(03)00148-X
  31. Takenaka, Y., Miki, M., and Yasudo, H., The effect of alpha tocopherol as an antioxidant on the oxidation membrane protein thiols induced by free radicals generated in different sites. Arch. Biochem. Biophys. 283, 344-350 (1991)
  32. Varier, P.S. Coscinium fenestratum, In: Indian Medicinal Plants, Compendium of 500 species, Orient Longman Ltd., Hyderabad, India, Vol. 2, pp. 191-193 (1994)
  33. Venukumar, M.R., and Latha, M.S., Effect of Coscinium fenestratum on hepatotoxicity in rats. Ind. J. Exp. Biol., 42, 792-797 (2004)
  34. Wohaieb, S.A. and Godin, D.V., Alterations in free radical tissue defense mechanism in STZ induced diabetes in rats. Effects of insulin treatment. Diabetes. 36, 1014-1018 (1987) https://doi.org/10.2337/diabetes.36.9.1014