Journal of Microbiology

  • 제43권1호
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  • Pages.49-53
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  • 2005
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Identification of Two-Component Regulatory Genes Involved in o-Xylene Degradation by Rhodococcus sp. Strain DK17

  • Kim, Doc-Kyu (Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Microbial Resources Bank, Microbial Genomics and Applications Center Korea Research Institute of Bioscience and Biotechnology) ;
  • Chae Jong-Chan (Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University) ;
  • Zylstra Gerben J. (Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University) ;
  • Sohn Ho-Yong (Department of Food and Nutrition, Andong National University) ;
  • Kwon, Gi-Seok (The School of Bioresource Science, Andong National University) ;
  • Kim, Eung-Bin (Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University)
  • 발행 : 2005.02.28
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초록

Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.

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참고문헌

  1. Asturias, J.A. and K.N. Timmis. 1993. Three different 2,3-dihydroxy biphenyl-1,2-dioxygenase genes in the gram-positive polychlorobiphenyl-degrading bacterium Rhodococcus globerulus P6. J. Bacteriol. 175, 4631-4640 https://doi.org/10.1128/jb.175.15.4631-4640.1993
  2. Choi, K.Y., D. Kim, S.-C. Koh, J.-S. So, J.-S. Kim, and E. Kim. 2004. Molecular cloning and identification of a novel oxygenase gene specifically induced during the growth of Rhodococcus sp. strain T104 on limonene. J. Microbiol. 42, 160-162
  3. Finnerty, W.R. 1992. The biology and genetics of the genus Rhodococcus. Annu. Rev. Microbiol. 46, 193-218 https://doi.org/10.1146/annurev.mi.46.100192.001205
  4. Haddad, S., D.M. Eby, and E.L. Neidle. 2001. Cloning and expression of the benzoate dioxygenase genes from Rhodococcus sp. strain 19070. Appl. Environ. Microbiol. 67, 2507-2514 https://doi.org/10.1128/AEM.67.6.2507-2514.2001
  5. Kesseler, M., E.R. Dabbs, B. Averhoff, and G. Gottschalk. 1996. Studies on the isopropylbenzene 2,3-dioxygenase and the 3-isopropylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of Rhodococcus erythropolis BD2. Microbiology 142, 3241-3251 https://doi.org/10.1099/13500872-142-11-3241
  6. Kim, D., Y-S. Kim, J.W. Jung, G.J. Zylstra, Y.M. Kim, S.-K. Kim, and E. Kim. 2003. Regioselective oxidation of xylene isomers by Rhodococcus sp. strain DK17. FEMS Microbiol. 223, 211-214 https://doi.org/10.1016/S0378-1097(03)00379-3
  7. Kim, D., Y-S. Kim, S-K. Kim, S.W. Kim, G.J. Zylstra, Y.M. Kim, and E. Kim. 2002. Monocyclic aromatic hydrocarbon degradation by Rhodococcus sp. strain DK17. Appl. Environ. Microbiol. 68, 3270-3278 https://doi.org/10.1128/AEM.68.7.3270-3278.2002
  8. Kim, D., J-C. Chae, G. J. Zylstra, Y-S. Kim, S-K. Kim, M. H. Nam, Y. M. Kim, and E. Kim. 2004. Identification of a novel dioxygenase involved in metabolism of o-xylene, toluene, and ethylbenzene by Rhodococcus sp. strain DK17. Appl. Environ. Microbiol. 70, 7086-7092 https://doi.org/10.1128/AEM.70.12.7086-7092.2004
  9. Kim, D., J-C. Chae, J. Y. Jang, G. J. Zylstra, Y. M. Kim, B. S. Kang, and E. Kim. 2005. Functional characterization and molecular modeling of methylcatechol 2,3-dioxygenase from o-xylenedegrading Rhodococcus sp. strain DK17. Biochem. Biophys. Res. Commun. 326, 880-886 https://doi.org/10.1016/j.bbrc.2004.11.123
  10. Labbe, D., J. Garnon, and P.C. Lau. 1997. Characterization of the genes encoding a receptor-like histidine kinase and a cognate response regulator from a biphenyl/polychlorobiphenyl-degrading bacterium, Rhodococcus sp. strain M5. J. Bacteriol. 179, 2772-2776 https://doi.org/10.1128/jb.179.8.2772-2776.1997
  11. Larkin, M.J., R. De Mot, L.A. Kulakov, and I. Nagy. 1998. Applied aspects of Rhodococcus genetics. Antonie Van Leeuwenhoek. 74, 133-153 https://doi.org/10.1023/A:1001776500413
  12. Mahenthiralingam, E. 1998. Extraction of RNA from mycobacteria. Methods Mol. Biol. 101, 65-75
  13. Parkinson, J.S. and E.C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26, 71-112 https://doi.org/10.1146/annurev.ge.26.120192.000443
  14. Sakai, M., K. Miyauchi, N. Kato, E. Masai, and M. Fukuda. 2003. 2-Hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 69, 427-433 https://doi.org/10.1128/AEM.69.1.427-433.2003
  15. Stanier, R.Y., N.J. Palleroni, and M. Duodoroff. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43, 159-271 https://doi.org/10.1099/00221287-43-2-159
  16. Stecker, C., A. Johann, C. Herzberg, B. Averhoff, and G. Gottschalk. 2003. Complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of Rhodococcus erythropolis BD2. J. Bacteriol. 185, 5269-5274 https://doi.org/10.1128/JB.185.17.5269-5274.2003
  17. Stock, A.M., V.L. Robinson, and P.N. Goudreau. 2000. Two-component signal transduction. Annu. Rev. Biochem. 69, 183-215 https://doi.org/10.1146/annurev.biochem.69.1.183
  18. Takeda, H., A. Yamada, K. Miyauchi, E. Masai, and M. Fukuda. 2004. Characterization of transcriptional regulatory genes for biphenyl degradation in Rhodococcus sp. strain RHA1. J. Bacteriol. 186, 2134-2146 https://doi.org/10.1128/JB.186.7.2134-2146.2004
  19. Volz, K. 1993. Structural conservation in the CheY superfamily. Biochemistry 32, 11741-11753 https://doi.org/10.1021/bi00095a001
  20. Walker, J.E., M. Saraste, M.J. Runswick, and N.J. Gay. 1982. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1, 945-951
  21. Wang, Y., J. Garnon, D. Labbe, H. Bergeron, and P. C. Lau. 1995. Sequence and expression of the bpdC1C2BADE genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by Rhodococcus sp. M5. Gene 164, 117-122 https://doi.org/10.1016/0378-1119(95)00448-F
  22. Warhurst, A.M. and C.A. Fewson. 1994. Biotransformations catalyzed by the genus Rhodococcus. Crit. Rev. Biotechnol. 14, 29-73 https://doi.org/10.3109/07388559409079833