Bulletin of the Korean Chemical Society

  • 제26권2호
  • /
  • Pages.278-280
  • /
  • 2005
  • /
  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
권호 표지
DOI QR코드

DOI QR Code

Nitrogen Isotope Labeled Tetraheme Cytochrome c3 on a Defined Medium

  • Kim, Andre (Department of Chemistry and Center for Innovative Bio-physio Sensor Technology, Pusan National University) ;
  • Park, Jang-Su (Department of Chemistry and Center for Innovative Bio-physio Sensor Technology, Pusan National University)
  • 발행 : 2005.02.20
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

To obtain cytochrome $c_3$ labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with $N_2$ and under pH control. DvMF was able to go on a defined medium without natural products. The composition of medium containing a small amount of $NH_4C$l as sole nitrogen source was established. Then, uniformly $^{15}N$labeled cytochrome $c_3$ was obtained during the culture of DvMF in a defined medium with $^{15}NH_4$Cl; it was confirmed by $^1H-^{15}N$ HMQC.

키워드

참고문헌

  1. Postagte, J. R. The Sulphate-Reducing Bacteria, 2nd ed.; Cambridge University: Cambridge, UK., 1984
  2. Tamura, A.; Kawate, T.; Ogata, M.; Yagi, T. J. Biochem. (Tokyo) 1988, 104, 722-726
  3. Yagi, T.; Inoguchi, H.; Kimura, K. Acc. Chem. Res. 1983, 16, 2-7 https://doi.org/10.1021/ar00085a001
  4. Higuchi, Y.; Kusunoki, M.; Matsuura, Y.; Yasuoka, N.; Kakudo, M. J. Mol. Biol. 1984, 172, 109-139 https://doi.org/10.1016/0022-2836(84)90417-0
  5. Niki, K.; Kobayashi, Y.; Matsuda, H. J. Electronal. Chem. 1984, 178, 333-341 https://doi.org/10.1016/S0022-0728(84)80011-X
  6. Fan, K.; Akutsu, H.; Kyogoku, Y.; Niki, N. Biochemistry 1990, 29, 2257-2263 https://doi.org/10.1021/bi00461a008
  7. Turner, D. L.; Salgueiro, C. A.; LeGall, J.; Xavier, A. V. Eur. J. Biochem. 1992, 210, 931-936 https://doi.org/10.1111/j.1432-1033.1992.tb17497.x
  8. Marion, D.; Kay, L. E.; Sparks, S. W.; Torchia, D. A.; Bax, A. J. Am. Chem. Soc. 1989, 111, 1515-1517 https://doi.org/10.1021/ja00186a066
  9. Gooley, P. R.; Caffrey, M. S.; Cusanovich, M. A.; Mackenzie, N. E. Biochemistry 1990, 29, 2278-2290 https://doi.org/10.1021/bi00461a011
  10. Li, M.; Park, D. S.; Park, J.-S. Bull. Korean Chem. Soc. 2003, 24(10), 1427-1428 https://doi.org/10.5012/bkcs.2003.24.10.1427
  11. Kim, A.; Jeong, I.-C.; Shim, Y.-B.; Kang, S.-W.; Park, J.-S. Bull. Korean Chem. Soc. 2001, 22(11), 1197-1201
  12. Kobayashi, K.; Skyring, G. W. J. Gen. Appl. Microbiol. 1982, 28, 45-54 https://doi.org/10.2323/jgam.28.45
  13. Park, J.-S.; Kang, S.-W. J. Biochem. Mol. Biol. 1995, 28(5), 433-436
  14. Bax, A.; Griffey, R. H.; Hawkins, B. L. J. Magn. Reson. 1983, 55, 301-315
  15. Reis, M. A. M.; Almeida, J. S.; Lemos, P. C.; Carrondo, M. J. T. Biotechnol. Bioeng. 1992, 40, 593-600 https://doi.org/10.1002/bit.260400506
  16. Tang, I. C.; Okos, M. R.; Yang, S. T. Biotechnol. Bioeng. 1989, 34, 1063-1074 https://doi.org/10.1002/bit.260340807
  17. Stans, A. J. M.; Hansen, T. A.; Skyring, G. W. FEMS Microbiol. Ecol. 1985, 31, 11-15 https://doi.org/10.1016/0378-1097(85)90041-2
  18. Khosrovi, B.; Miller, J. D. A. Plant. Soil. 1975, 43, 171-187 https://doi.org/10.1007/BF01928484
  19. Tsuji, K.; Yagi, T. Arch. Microbiol. 1980, 125, 35-42 https://doi.org/10.1007/BF00403195
  20. Shinkai, W.; Hase, T.; Yagi, T.; Matsubara, H. J. Biochem. (Tokyo) 1980, 87, 1747-1756