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http://dx.doi.org/10.5012/bkcs.2005.26.2.278

Nitrogen Isotope Labeled Tetraheme Cytochrome c3 on a Defined Medium  

Kim, Andre (Department of Chemistry and Center for Innovative Bio-physio Sensor Technology, Pusan National University)
Park, Jang-Su (Department of Chemistry and Center for Innovative Bio-physio Sensor Technology, Pusan National University)
Publication Information
Bulletin of the Korean Chemical Society / v.26, no.2, 2005 , pp. 278-280 More about this Journal
Abstract
To obtain cytochrome $c_3$ labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with $N_2$ and under pH control. DvMF was able to go on a defined medium without natural products. The composition of medium containing a small amount of $NH_4C$l as sole nitrogen source was established. Then, uniformly $^{15}N$labeled cytochrome $c_3$ was obtained during the culture of DvMF in a defined medium with $^{15}NH_4$Cl; it was confirmed by $^1H-^{15}N$ HMQC.
Keywords
Cytochrome $c_3$; $^1H-^{15}N$ HMQC; Stable isotope;
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