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Effects of Adsorption Condition on Fat-binding Characteristics of Chitosan

흡착조건이 키토산의 지방질 흡착 특성에 미치는 영향

  • LEE Keun-Tai (Division of Food Science and Biotechnology, Pukyong National University) ;
  • SONG Ho-Su (Division of Food Science and Biotechnology, Pukyong National University) ;
  • PARK Seong-Min (Coorperative Laboratoy Center of Pukyong National University) ;
  • KANG Ok-Ju (Division of Life science of Kyungnam University) ;
  • CHEONG Hyo-Sook (Division of Life science of Kyungnam University)
  • 이근태 (부경대학교 식품생명공학부) ;
  • 송호수 (부경대학교 식품생명공학부) ;
  • 박성민 (부경대학교 공동실험실습관) ;
  • 강옥주 (경남대학교 생명과학부) ;
  • 정효숙 (경남대학교 생명과학부)
  • Published : 2004.10.01

Abstract

To study the lipid adsorption characteristic of chitosans with different molecular weights and the degrees of deacetylation, in vitro test and near-infrared (NIR) spectroscopic analysis have been performed for the measurement of lipid adsorption characteristics of chitosan. The degrees of deacetylation in chitosans were $70{\%},\;85{\%}\;and\;92{\%}$ at different deacetylation times (1 hr, 2 hrs, 3 hrs), respectively. The molecular weight of each chitosan was controlled by enzymatic hydrolysis, and then the molecular weight of the chitosan was 4 kDa. The bulk density, water holding capacity and fat binding capacity of each chitosan powder were $96.2-504.0{\%},\;374.4-1217.9{\%},\;and\;307.0-659.3{\%}$, respectively. The higher molecular weight of chitosan was exhibited the lower bulk density and the higher water and fat binding capacities. Bindinf capacities of chitosan powders to bile salts, cholesterol and linoleic acid were $41.2-63.3{\%},\;40.8-67.4{\%},\;42.6-72.6{\%}$, respectively. In NIR spectrum of lipid adsorbed chitosan the occurrence static eletronical binding between chitosan and lipid was identified by NIR spectrum peak induced from combination of carboxylic group in lipid and amino group in chitosan. In conclusion, the higher degree of deacetylation and molecular weight of chitosan showed the higher lipid binding capacity and the lipid adsorption of chitosan were occurred by combination of carboxylic group in lipids and amino group in chitosan.

Keywords

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