동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제18권4호
  • /
  • Pages.1129-1133
  • /
  • 2004
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

Human Immunodeficiency Virus Type Ⅰ에 대한 음나무 추출물의 억제활성

The Extracts of Kalopanax pictus Nakai. for Inhibitory Effects on HIV-1 and Its Essential Enzymes

  • 유영법 (한국한의학연구원 의료연구부) ;
  • 심범상 (경희대학교 한의과대학 병리학교실) ;
  • 안규석 (경희대학교 한의과대학 병리학교실) ;
  • 최승훈 (WHO 서태평양지역사무국 전통의약담당) ;
  • 박종철 (순천대학교 한약자원학과) ;
  • ;
  • Yu Young Beob (Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Shim Bum Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Ahn Kyoo Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Choi Seung Hoon (WHO Western Pacific Regional Office) ;
  • Park Jong Cheol (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Miyashiro H. (Research Institute for Wakan Yaku, Toyama Medical and Pharmaceutical University) ;
  • Hattori M. (Research Institute for Wakan Yaku, Toyama Medical and Pharmaceutical University)
  • 발행 : 2004.08.01
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초록

For the purpose of developing new anti-HIV agents from natural sources, the extracts of Kalopanax pictus were tested for their inhibitory effects on HIV-1 replication and its essential enzymes as the reverse transcriptase (RT). protease and α-glucosidase. In the assay of HIV-1-infected human T-cell line, water extracts of stem and leafstalk inhibited the HIV-1-induced cytopathic effects with Ie (inhibitory concentration) of 25 and 50㎍/㎖, respectively. Moreover water extracts (100㎍/㎖) of stem and leafstalk showed strong activity of 80% and 90% on anti-HIV-1 RT using Enzyme Linked Oligonucleotide Sorbent Assay (ELOSA) method. In the HIV-1 protease inhibition assay, aqueous stem extract inhibited the activity of the enzyme to cleave an oligopeptide, resembling one of the cleavage sites in the viral polyprotein which can only be processed by HIV-1 protease with 58%, but no glucosidase inhibitory activities. We found out this result, for these samples it is possible that the inhibition of the viral replication in vitro is due to the inhibition at least one of RT and protease. It would be of great interest to identify the compounds which are responsible for this inhibition, since all therapeutically useful agent up to date are RT, PR and α-glucosidase inhibitors.

키워드

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