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The Extracts of Kalopanax pictus Nakai. for Inhibitory Effects on HIV-1 and Its Essential Enzymes  

Yu Young Beob (Department of Medical Research, Korea Institute of Oriental Medicine)
Shim Bum Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Ahn Kyoo Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Choi Seung Hoon (WHO Western Pacific Regional Office)
Park Jong Cheol (Department of Oriental Medicine Resources, Sunchon National University)
Miyashiro H. (Research Institute for Wakan Yaku, Toyama Medical and Pharmaceutical University)
Hattori M. (Research Institute for Wakan Yaku, Toyama Medical and Pharmaceutical University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.4, 2004 , pp. 1129-1133 More about this Journal
Abstract
For the purpose of developing new anti-HIV agents from natural sources, the extracts of Kalopanax pictus were tested for their inhibitory effects on HIV-1 replication and its essential enzymes as the reverse transcriptase (RT). protease and α-glucosidase. In the assay of HIV-1-infected human T-cell line, water extracts of stem and leafstalk inhibited the HIV-1-induced cytopathic effects with Ie (inhibitory concentration) of 25 and 50㎍/㎖, respectively. Moreover water extracts (100㎍/㎖) of stem and leafstalk showed strong activity of 80% and 90% on anti-HIV-1 RT using Enzyme Linked Oligonucleotide Sorbent Assay (ELOSA) method. In the HIV-1 protease inhibition assay, aqueous stem extract inhibited the activity of the enzyme to cleave an oligopeptide, resembling one of the cleavage sites in the viral polyprotein which can only be processed by HIV-1 protease with 58%, but no glucosidase inhibitory activities. We found out this result, for these samples it is possible that the inhibition of the viral replication in vitro is due to the inhibition at least one of RT and protease. It would be of great interest to identify the compounds which are responsible for this inhibition, since all therapeutically useful agent up to date are RT, PR and α-glucosidase inhibitors.
Keywords
Kalopanax pictus; anti HIV-1; reversetranscriptase; protease; α-glucosidase;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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