십전대보탕이 신경교세포의 산화적 손상에 미치는 영향

Effects of Sibjeondaebo-Tang on Oxidative Stress of C6 Glial Cells

  • 류지용 (원광대학교 한의과대학 심계내과학교실) ;
  • 윤종민 (원광대학교 한의과대학 심계내과학교실, 원광대학교 한의학전문대학원 제3의학과) ;
  • 조광호 (원광대학교 한의학전문대학원 제3의학과) ;
  • 문병순 (원광대학교 한의과대학 심계내과학교실, 원광대학교 한의학전문대학원 제3의학과)
  • Ryu Ji Yong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Yun Jong Min (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University, Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho Kwang Ho (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Moon Byung Soon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University, Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 발행 : 2004.08.01

초록

Zinc ion has both essential and toxic effects on mammalian cells. The results demonstrated that the ability of zinc to act as an inducer of apoptosis in C6 glial cells. Incubation with 0.2 mM ZnCl₂ caused cell death that was characterized as apoptosis by internucleosomal DNA fragmentation, formation of apoptotic bodies, nuclear fragmentation and breakdown of the mitochondrial membrane potential. ZnCl₂-induced apoptosis of C6 glial cells was prevented by the addition of Sibjeondaebo-Tang and antioxidants including reduced glutathione, N-acetyl-L-cysteine. We further confirmed that ZnCl₂ decreased the intracellular levels of GSH as well as generation of H₂O₂ in C6 glial cells. In 2D-electrophoresis, computer-assisted comparative analysis of the respective silver stained spot patterns revealed 3 groups with strongly decreased intensity by ZnCl₂. Whereas, 3 groups with increasing intensity were recovered by Sibjeondaebo-Tang. These results suggest that Sibjeondaebo-Tang may function as an antioxidant against free radicals and be applicable to the treatment of brain cells against oxidative stress.

키워드

참고문헌

  1. 노인보건 조유향
  2. 노인의학 배철영;이영진
  3. 中西醫臨床老年病學 杜建主
  4. 臨床中醫腦病學 施杞;周康
  5. 太平惠民和劑局方 陳師文
  6. 慶熙韓醫大論文集 v.1 no.1 十全大補湯 Extract 투여가 Rat의 성장에 미치는 영향 金吉萱(등)
  7. 十全大補湯이 生肌作用에 미치는 影響 辛美香
  8. 十全大補湯加味方이 Hydrogen Peroxide에 의해 損傷된 培養 脊髓感覺細胞에 미치는 影響 韓東元
  9. 十全大補湯 煎湯엑기스가 생쥐의 細胞性 및 體液性免疫反應에 미치는 影響 金在燮
  10. 十全大補湯加味方이 마우스의 貪食細胞機能 및 腫瘍 免疫反應에 미치는 영향 辛京兒
  11. 十全大補湯의 抗酸化作用에 關한 硏究 許埈寧
  12. J Neurosci v.15 Mitochondria production of reactive oxygen species in cortical neurons following exposure to N-methyl-D-aspartate Dugan L.L;Sensi S.L;Canzoniero L.M.T;Choi D.W.
  13. 黃帝內經素問 洪元植(編)
  14. 類經(下) 張介賓
  15. 惠和醫學 v.6 no.1 內經의 腦學說에 대한 文獻的 考察 許美晶(外)
  16. 中醫雜誌 v.38 no.10 謝海洲治療腦萎縮經驗 郭字鵬(등)
  17. 難經入門 최승훈
  18. 한의학 기초 이론 羅昌洙(등)
  19. 韓方處方解說 矢敷導明
  20. 中醫方劑學 許濟群;王綿之
  21. Biochem Biophys Res Commun v.187 Inhibition of apoptosis by zinc. A reappraisal Barbieri D;Troiano L;Grassilli E;Agnesini C;Cristofalo E;Monit D;Capri M; Cossarizza A;Franceshi C.
  22. J Cell Biol v.141 The regulation of reactive oxygen species production during programmed cell death Shirlee Tan;Yutaka Sagara;Yuanbin Liu.
  23. J Biol Chem v.272 Epidermal growth factor (EGF)-induced generation of hydrogen peroxide Bae Y.S;Kang S.W;Seo M.S;Bains I.C;Tekle E;Chock P.B.;Rhee S.G.
  24. Science v.270 Requirement for Generation of $H_2O_2$ for Platelet Derived Growth Factor Signal Transduction Sundaresan M;Yu Z.Y;Ferrans V.J;Irani K.;Finkel T.
  25. Biochem Biophys Res Commun v.181 Apoptosis and DN degeneration induced by 1-methyl-4-phenylpyridinium in neurons Dipasquale B;Marini A.M;Youle R.J.
  26. J Cereb Blood Flow Metab v.22 no.4 Activation of mitochondrial ATP-dependent potassium channels protects neurons against ischemia-induced death by a mechanism involving suppression of Bax translocation and cytochrome c release Liu D;Lu C;Wan R;Auyeung W.W;Mattson M.P.
  27. Biol Chem v.277 no.12 MEK kinase 1 induces mitochondrial permeability transition leading to apoptosis independent of cytochrome c release Gibson E.M;Henson E.S;Villanueva J;Gibson S.B.
  28. Proc Natl Acad Sci USA v.98 no.26 Granzyme B. can cause mitochondrial depolarization and cell death in the absence of BID, BAX and BAK Thomas D.A;Scorrano L;Putcha G.V;Korsmeyer S.J;Ley T.J.
  29. Hum Mol Genet v.10 no.21 Bcl-2-L-10, a novel anti-apoptotic member of the Bcl-2 family, blocks apoptosis in the mitochondria death pathway but not in the death receptor pathway Zhang H;Holzgreve W;De Geyter C.