Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Induction of Hariy Root and Bioreactor Culture of Lycium chinense

구기자나무 (Lycium chinense)의 모상근 유도 및 생물반응기 배양

  • 배기화 (마이크로프랜츠 부설연구소) ;
  • 김윤수 (중앙대학교 인삼산업연구센터) ;
  • 정재훈 (국립산림과학원 생물공학과) ;
  • 김영선 (남도대학 약용자원원예개발과) ;
  • 최용의 (강원대학교 산림과학대학 산림자원부) ;
  • 윤의수 (공주대학교 생물학과)
  • Published : 2004.12.01
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Abstract

This article was conducted to induce the transgenic hairy roots and determine the effect of culture conditions on optimum growth of hairy roots by Agrobacterium rhizogenes strain, 15834 in Lycium chinense Miller. Hairy roots of L. chinense Miller. were induced from leaf segments by co-cultivation with A. rhizogenes. When the hairy roots were cultured in various MS medium strength and sucrose concentrations, the highest growth of hairy roots was observed in half-strength MS media supplemented with 3% sucrose, respectively. In air lift bioreactor cultures, the liquid medium contained with 1/2 MS and 3% sucrose was also the best for optimum growth of hairy roots.

구기자나무의 잎 절편을 A. rhizogenes strain, 15834와 공동 배양하여 유도된 모상근의 최적배양조건을 결정하기 위하여 MS배지와 sucrose의 농도를 다양하게 처리한 결과, 1/2 MS 배지에서 생체중 (12.3 g)과 건물중 (1.4 g)으로 가장 높게 나타났으며, 탄소원으로 사용된 sucrose에서는 3% 농도에서 생체중과 건물중이 가장 양호하게 나타났다. 이와 같이 결정된 MS농도와 sucrose의 농도를 이용하여 대량배양을 하고자 10 L의 air lift bioreactor에서 대량 배양한 결과 역시 1/2 MS와 3% sucrose의 농도를 사용한 처리구에서 가장 높은 생체중과 건물중의 증가를 보였다. 하지만, 1/2 MS에 5% sucrose를 처리한 bioreactor배양에서도 3% sucrose처리에서와 마찬가지로 생체중과 건물중이 높게 나타났다.

Keywords

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