Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Design of Bacterial Vector Systems for the Production of Recombinant Proteins in Escherichia coli

  • Mergulhao (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico) ;
  • Filipe J.M. (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico) ;
  • Gabriel A. Monteiro (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico) ;
  • Joaquim M.S. Cabral (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico) ;
  • M. Angela Taipa (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico)
  • Published : 2004.02.01
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Abstract

More than twenty years have passed since the approval of the first recombinant DNA product for therapeutic use (recombinant human insulin, 1982). However, the biotechnology industry is still facing a shortage of manufacturing capacity due to the increasing demand of therapeutic proteins. This demand has prompted the search for a growing number of biological production systems but, nevertheless, the Gram-negative bacterium Escherichia coli remains one of the most attractive production hosts. This review highlights the most important features and developments of plasmid vector design, emphasizing the different reported strategies for improving the expression and secretion of heterologous proteins using the cellular machinery of E. coli.

Keywords

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