Design of Bacterial Vector Systems for the Production of Recombinant Proteins in Escherichia coli |
Mergulhao
(Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico)
Filipe J.M. (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico) Gabriel A. Monteiro (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico) Joaquim M.S. Cabral (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico) M. Angela Taipa (Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico) |
1 | Abrahmsen, L., T. Moks, B. Nilsson, and M. Uhlen. 1986. Secretion of heterologous gene products to the culture medium of Escherichia coli. Nucleic Acids Res. 14: 7487- 7500 |
2 | Andersson, H. and G. von Heijne. 1991. A 30-residue-long “export initiation domain” adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli. Proc. Natl. Acad. Sci. USA 88: 9751- 9754 |
3 | Balbas, P. 2001. Understanding the art of producing protein and nonprotein molecules in Escherichia coli. Mol. Biotechnol. 19: 251-267 |
4 | Balbas, P. and F. Bolivar. 1990. Design and construction of expression plasmid vectors in Escherichia coli. Methods Enzymol. 185: 14-37 |
5 | Balbas, P. and G. Gosset. 2001. Chromosomal editing in Escherichia coli. Vectors for DNA integration and excision. Mol. Biotechnol. 19: 1-12 |
6 | Barth, S., M. Huhn, B. Matthey, A. Klimka, E. A. Galinski, and A. Engert. 2000. Compatible-solute-supported periplasmic expression of functional recombinant proteins under stress conditions. Appl. Environ. Microbiol. 66: 1572-1579 |
7 | Bayer, M., R. Iberer, K. Bischof, E. Rassi, E. Stabentheiner, G. Zellnig, and G. Koraimann. 2001. Functional and mutational analysis of p19, a DNA transfer protein with muramidase activity. J. Bacteriol. 183: 3176-3183 |
8 | Berg, O. G. and C. G. Kurland. 1997. Growth rate-optimised tRNA abundance and codon usage. J. Mol. Biol. 270: 544- 550 |
9 | Cebolla, A., J. L. Royo, V. De Lorenzo, and E. Santero. 2002. Improvement of recombinant protein yield by a combination of transcriptional amplification and stabilization of gene expression. Appl. Environ. Microbiol. 68: 5034- 5041 |
10 | Chatwin, H. M. and D. K. Summers. 2001. Monomer-dimer control of the ColE1 P(cer) promoter. Microbiol. 147: 3071- 3081 |
11 | Cornelis, P., J. C. Sierra, A. Lim, Jr., A. Malur, S. Tungpradabkul, H. Tazka, A. Leitao, C. V. Martins, C. di Perna, L. Brys, P. De Baetseller, and R. Hamers. 1996. Development of new cloning vectors for the production of immunogenic outer membrane fusion proteins in Escherichia coli. Biotechnol. (N Y). 14: 203-208 DOI ScienceOn |
12 | del Solar, G., R. Giraldo, M. J. Ruiz-Echevarria, M. Espinosa, and R. Diaz-Orejas. 1998. Replication and control of circular bacterial plasmids. Microbiol. Mol. Biol. Rev. 62: 434-464 |
13 | del Tito, B. J., Jr., J. M. Ward, J. Hodgson, C. J. Gershater, H. Edwards, L. A. Wysocki, F. A. Watson, G. Sathe, and J. F. Kane. 1995. Effects of a minor isoleucyl tRNA on heterologous protein translation in Escherichia coli. J. Bacteriol. 177: 7086-7091 |
14 | Dong, H., L. Nilsson, and C. G. Kurland. 1995. Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction. J. Bacteriol. 177: 1497-1504 |
15 | Gavit, P. and M. Better. 2000. Production of antifungal recombinant peptides in Escherichia coli. J. Biotechnol. 79: 127-136 |
16 | Grossman, T. H., E. S. Kawasaki, S. R. Punreddy, and M. S. Osburne. 1998. Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene 209: 95-103 |
17 | Guarente, L., G. Lauer, T. M. Roberts, and M. Ptashne. 1980. Improved methods for maximizing expression of a cloned gene: A bacterium that synthesizes rabbit betaglobin. Cell 20: 543-553 |
18 | Hannig, G. and S. C. Makrides. 1998. Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol. 16: 54-60 |
19 | Hew, Y., C. Lau, Z. Grzelczak, and F. W. Keeley. 2000. Identification of a GA-rich sequence as a proteinbinding site in the 3-untranslated region of chicken elastin mRNA with a potential role in the developmental regulation of elastin mRNA stability. J. Biol. Chem. 275: 24857- 24864 |
20 | Holm, L. 1986. Codon usage and gene expression. Nucleic Acids Res. 14: 3075-3087 |
21 | Ignatova, Z., A. Mahsunah, M. Georgieva, and V. Kasche. 2003. Improvement of posttranslational bottlenecks in the production of penicillin amidase in recombinant Escherichia coli strains. Appl. Environ. Microbiol. 69: 1237-1245 |
22 | Ikemura, T. 1981. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes. J. Mol. Biol. 146: 1- 21 |
23 | Ikemura, T. 1981. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational system. J. Mol. Biol. 151: 389-409 |
24 | Jeong, K. J. and S. Y. Lee. 2003. Enhanced production of recombinant proteins in Escherichia coli by filamentation suppression. Appl. Environ. Microbiol. 69: 1295-1298 |
25 | Joly, J. C., W. S. Leung, and J. R. Swartz. 1998. Overexpression of Escherichia coli oxidoreductases increases recombinant insulin-like growth factor-I accumulation. Proc. Natl. Acad. Sci. USA 95: 2773-2777 |
26 | Kajava, A. V., S. N. Zolov, A. E. Kalinin, and M. A. Nesmeyanova. 2000. The net charge of the first 18 residues of the mature sequence affects protein translocation across the cytoplasmic membrane of gram-negative bacteria. J. Bacteriol. 182: 2163-2169 |
27 | Kane, J. F. 1995. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 6: 494-500 DOI PUBMED ScienceOn |
28 | Keefe, A. D., D. S. Wilson, B. Seelig, and J. W. Szostak. 2001. One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBPTag. Protein Expr. Purif. 23: 440-446 |
29 | Kelley, B. D. 2001. Biochemical engineering: Bioprocessing of therapeutic proteins. Curr. Opin. Biotechnol. 12: 173- 174 DOI PUBMED ScienceOn |
30 | Kim, J., J. Luirink, and D. A. Kendall. 2000. SecB dependence of an exported protein is a continuum influenced by the characteristics of the signal peptide or early mature region. J. Bacteriol. 182: 4108-4112 |
31 | Kurokawa, Y., H. Yanagi, and T. Yura. 2000. Overexpression of protein disulfide isomerase DsbC stabilizes multipledisulfide- bonded recombinant protein produced and transported to the periplasm in Escherichia coli. Appl. Environ. Microbiol. 66: 3960-3965 |
32 | Lee, S., I. Kim, D. Kim, K. Bae, and S. Byun. 1998. High level secretion of recombinant staphylokinase into periplasm of Escherichia coli. Biotechnol. Lett. 20: 113-116 |
33 | Monzavi-Karbassi, B., G. Cunto-Amesty, P. Luo, and T. Kieber-Emmons. 2002. Peptide mimotopes as surrogate antigens of carbohydrates in vaccine discovery. Trends Biotechnol. 20: 207-214 |
34 | Mujacic, M., K. W. Cooper, and F. Baneyx. 1999. Coldinducible cloning vectors for low-temperature protein expression in Escherichia coli: Application to the production of a toxic and proteolytically sensitive fusion protein. Gene 238: 325-332 |
35 | Murby, M., P. A. Nygren, H. Rondahl, U. Hellman, S. O. Enfors, and M. Uhlen. 1991. Differential degradation of a recombinant albumin-binding receptor in Escherichia coli. Eur. J. Biochem. 199: 41-46 |
36 | Nielsen, P. E. 1999. Applications of peptide nucleic acids. Curr. Opin. Biotechnol. 10: 71-75 DOI PUBMED ScienceOn |
37 | Nilsson, B. and L. Abrahmsen. 1990. Fusions to staphylococcal protein A. Methods Enzymol. 185: 144-161 |
38 | Nilsson, B., G. Forsberg, T. Moks, M. Hartmanis, and M. Uhlen. 1992. Fusion proteins in biotechnology. Curr. Opin. Biotechnol. 3: 363-369 |
39 | Nilsson, J., S. Stahl, J. Lundeberg, M. Uhlen, and P. A. Nygren. 1997. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr. Purif. 11: 1-16 |
40 | Polyak, S. W., G. Forsberg, B. E. Forbes, K. A. McNeil, S. E. Aplin, and J. C. Wallace. 1997. Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site-specific cleavage. Protein Eng. 10: 615-619 |
41 | Pugsley, A. P., O. Francetic, O. M. Possot, N. Sauvonnet, and K. Hardie. 1997. Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-neagtive bacteria - a review. Gene 192: 13-19 DOI ScienceOn |
42 | Rinas, U. and J. Bailey. 1992. Protein compositional analysis of inclusion bodies produced in recombinant Escherichia coli. Appl. Microbiol. Biotechnol. 37: 609-614 |
43 | Robinson, M., R. Lilley, S. Little, J. S. Emtage, G. Yarranton, P. Stephens, A. Millican, M. Eaton, and G. Humphreys. 1984. Codon usage can affect efficiency of translation of genes in Escherichia coli. Nucleic Acids Res. 12: 6663-6671 |
44 | Rosenberg, A. H., E. Goldman, J. J. Dunn, F. W. Studier, and G. Zubay. 1993. Effects of consecutive AGG codons on translation in Escherichia coli, demonstrated with a versatile codon test system. J. Bacteriol. 175: 716-722 |
45 | Salmond, G. P. and P. J. Reeves. 1993. Membrane traffic wardens and protein secretion in gram-negative bacteria. Trends Biochem. Sci. 18: 7-12 |
46 | Sharpe, M. E., H. M. Chatwin, C. Macpherson, H. L. Withers, and D. K. Summers. 1999. Analysis of the CoIE1 stability determinant Rcd. Microbiol. 145: 2135-2144 |
47 | Shokri, A., A. M. Sanden, and G. Larsson. 2002. Growth rate-dependent changes in Escherichia coli membrane structure and protein leakage. Appl. Microbiol. Biotechnol. 58: 386-392 |
48 | Siegele, D. A. and J. C. Hu. 1997. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc. Natl. Acad. Sci. USA 94: 8168-8172 |
49 | Swamy, K. H. and A. L. Goldberg. 1982. Subcellular distribution of various proteases in Escherichia coli. J. Bacteriol. 149: 1027-1033 |
50 | Tang, J. and M. Hu. 1993. Production of human proinsulin in E. coli in a non-fusion form. Biotechnol. Lett. 15: 661- 666 |
51 | Thomas, J. G. and F. Baneyx. 1996. Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing heat-shock proteins. J. Biol. Chem. 271: 11141-11147 |
52 | Thomas, J. G. and F. Baneyx. 2000. ClpB and HtpG facilitate de novo protein folding in stressed Escherichia coli cells. Mol. Microbiol. 36: 1360-1370 |
53 | Tomoyasu, T., A. Mogk, H. Langen, P. Goloubinoff, and B. Bukau. 2001. Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol. Mol. Microbiol. 40: 397-413 |
54 | Gottesman, S. 1996. Proteases and their targets in Escherichia coli. Annu. Rev. Genet. 30: 465-506 |
55 | Wang, H., D. J. O’Mahony, D. J. McConnell, and S. Z. Qi. 1993. Optimization of the synthesis of porcine somatotropin in Escherichia coli. Appl. Microbiol. Biotechnol. 39: 324- 328 |
56 | Williams, D. P., D. Regier, D. Akiyoshi, F. Genbauffe, and J. R. Murphy. 1988. Design, synthesis and expression of a human interleukin-2 gene incorporating the codon usage bias found in highly expressed Escherichia coli genes. Nucleic Acids Res. 16: 10453-10467 |
57 | Winter, J., P. Neubauer, R. Glockshuber, and R. Rudolph. 2001. Increased production of human proinsulin in the periplasmic space of Escherichia coli by fusion to DsbA. J. Biotechnol. 84: 175-185 |
58 | Batisson, I. and M. der Vartanian. 2000. Extracellular DsbA-insensitive folding of Escherichia coli heat-stable enterotoxin STa in vitro. J. Biol. Chem. 275: 10582-10589 |
59 | Fargo, D. C., J. E. Boynton, and N. W. Gillham. 1999. Mutations altering the predicted secondary structure of a chloroplast 5' untranslated region affect its physical and biochemical properties as well as its ability to promote translation of reporter mRNAs both in the Chlamydomonas reinhardtii chloroplast and in Escherichia coli. Mol. Cell. Biol. 19: 6980-6990 |
60 | Missiakas, D. and S. Raina. 1997. Protein misfolding in the cell envelope of Escherichia coli: New signaling pathways. Trends Biochem. Sci. 22: 59-63 |
61 | Gautam, A. and D. Bastia. 2001. A replication terminus located at or near a replication checkpoint of Bacillus subtilis functions independently of stringent control. J. Biol. Chem. 276: 8771-8777 |
62 | Rowe, D. C. and D. K. Summers. 1999. The quiescent-cell expression system for protein synthesis in Escherichia coli. Appl. Environ. Microbiol. 65: 2710-2715 |
63 | Summers, R. G., C. R. Harris, and J. R. Knowles. 1989. A conservative amino acid substitution, arginine for lysine, abolishes export of a hybrid protein in Escherichia coli. Implications for the mechanism of protein secretion. J. Biol. Chem. 264: 20082-20088 |
64 | Baker, K. N., M. H. Rendall, A. Patel, P. Boyd, M. Hoare, R. B. Freedman, and D. C. James. 2002. Rapid monitoring of recombinant protein products: A comparison of current technologies. Trends Biotechnol. 20: 149-156 |
65 | Fernandez, L. A., I. Sola, L. Enjuanes, and V. de Lorenzo. 2000. Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system. Appl. Environ. Microbiol. 66: 5024- 5029 |
66 | Pines, O. and M. Inouye. 1999. Expression and secretion of proteins in E. coli. Mol. Biotechnol. 12: 25-34 |
67 | Samuelsson, E., P. Jonasson, F. Viklund, B. Nilsson, and M. Uhlen. 1996. Affinity-assisted in vivo folding of a secreted human peptide hormone in Escherichia coli. Nat. Biotechnol. 14: 751-755 |
68 | Sandkvist, M. 2001. Biology of type II secretion. Mol. Microbiol. 40: 271-283 |
69 | Bonekamp, F. and K. F. Jensen. 1988. The AGG codon is translated slowly in E. coli even at very low expression levels. Nucleic Acids Res. 16: 3013-3024 |
70 | Canals, A., M. Ribo, A. Benito, M. Bosch, E. Mombelli, and M. Vilanova. 1999. Production of engineered human pancreatic ribonucleases, solving expression and purification problems, and enhancing thermostability. Protein Expr. Purif. 17: 169-181 |
71 | Jeong, K. J. and S. Y. Lee. 2001. Secretory production of human granulocyte colony-stimulating factor in Escherichia coli. Protein Expr. Purif. 23: 311-318 |
72 | Lee, S. Y. 1996. High cell-density culture of Escherichia coli. Trends Biotechnol. 14: 98-105 DOI PUBMED ScienceOn |
73 | Mergulhão, F., G. Monteiro, A. Kelly, M. Taipa, and J. Cabral. 2000. Recombinant human proinsulin: A new approach in gene assembly and protein expression. J. Microbiol. Biotechnol. 10: 690-693 |
74 | Simmons, L. C. and D. G. Yansura. 1996. Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli. Nat. Biotechnol. 14: 629-634 |
75 | Panayotatos, N. and K. Truong. 1985. Cleavage within an RNase III site can control mRNA stability and protein synthesis in vivo. Nucleic Acids Res. 13: 2227-2240 |
76 | Summers, D. 1998. Timing, self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol. Microbiol. 29: 1137-1145 |
77 | Nakai, K. and M. Kanehisa. 1991. Expert system for predicting protein localization sites in gram-negative bacteria. Proteins 11: 95-110 |
78 | Palacios, J. L., I. Zaror, P. Martinez, F. Uribe, P. Opazo, T. Socias, M. Gidekel, and A. Venegas. 2001. Subset of hybrid eukaryotic proteins is exported by the type I secretion system of Erwinia chrysanthemi. J. Bacteriol. 183: 1346- 1358 |
79 | Qiu, J., J. R. Swartz, and G. Georgiou. 1998. Expression of active human tissue-type plasminogen activator in Escherichia coli. Appl. Environ. Microbiol. 64: 4891- 4896 |
80 | Bonekamp, F., H. Dalboge, T. Christensen, and K. F. Jensen. 1989. Translation rates of individual codons are not correlated with tRNA abundances or with frequencies of utilization in Escherichia coli. J. Bacteriol. 171: 5812- 5816 |
81 | Bostrom, M. and G. Larsson. 2002. Introduction of the carbohydrate-activated promoter P(malK) for recombinant protein production. Appl. Microbiol. Biotechnol. 59: 231- 238 |
82 | Cooper, K. W. and F. Baneyx. 2001. Escherichia coli FtsH (HflB) degrades a membrane-associated TolAI-II-betalactamase fusion protein under highly denaturing conditions. Protein Expr. Purif. 21: 323-332 |
83 | Sozhamannan, S. and B. L. Stitt. 1997. Effects on mRNA degradation by Escherichia coli transcription termination factor Rho and pBR322 copy number control protein Rop. J. Mol. Biol. 268: 689-703 |
84 | Cranenburgh, R. M., J. A. Hanak, S. G. Williams, and D. J. Sherratt. 2001. Escherichia coli strains that allow antibioticfree plasmid selection and maintenance by repressor titration. Nucleic Acids Res. 29: E26 |
85 | Lederberg, J. 1998. Plasmid (1952-1997). Plasmid 39: 1-9 |
86 | Nygren, P. A., S. Stahl, and M. Uhlen. 1994. Engineering proteins to facilitate bioprocessing. Trends Biotechnol. 12: 184-188 |
87 | Yoon, S. and W. Kang. 1994. Fed-batch operation of recombinant Escherichia coli containing the trp promoter with controlled specific growth rate. Biotechnol. Bioeng. 43 |
88 | Bessette, P. H., F. Aslund, J. Beckwith, and G. Georgiou. 1999. Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc. Natl. Acad. Sci. USA 96: 13703-13708 |
89 | Chang, J. R., J. J. Choi, H. K. Kim, and S. T. Kwon. 2001. Purification and properties of Aquifex aeolicus DNA polymerase expressed in Escherichia coli. FEMS Microbiol. Lett. 201: 73-77 |
90 | Cubarsi, R., M. M. Carrio, and A. Villaverde. 2001. In situ proteolytic digestion of inclusion body polypeptides occurs as a cascade process. Biochem. Biophys. Res. Commun. 282: 436-441 |
91 | Manosroi, J., C. Tayapiwatana, F. Gotz, R. G. Werner, and A. Manosroi. 2001. Secretion of active recombinant human tissue plasminogen activator derivatives in Escherichia coli. Appl. Environ. Microbiol. 67: 2657-2664 |
92 | Swartz, J. R. 2001. Advances in Escherichia coli production of therapeutic proteins. Curr. Opin. Biotechnol. 12: 195-201 DOI PUBMED ScienceOn |
93 | Burgess, R. R. and N. E. Thompson. 2002. Advances in gentle immunoaffinity chromatography. Curr. Opin. Biotechnol. 13: 304-308 |
94 | Chang, S. G., D. Y. Kim, K. D. Choi, J. M. Shin, and H. C. Shin. 1998. Human insulin production from a novel mini-proinsulin which has high receptor-binding activity. Biochem. J. 329: 631-635 |
95 | Economou, A. 1999. Following the leader: Bacterial protein export through the Sec pathway. Trends Microbiol. 7: 315- 320 |
96 | Jordi, B. J., T. A. Owen-Hughes, C. S. Hulton, and C. F. Higgins. 1995. DNA twist, flexibility and transcription of the osmoregulated proU promoter of Salmonella typhimurium. EMBO J. 14: 5690-5700 |
97 | Andersen, D. C. and L. Krummen. 2002. Recombinant protein expression for therapeutic applications. Curr. Opin. Biotechnol. 13: 117-123 |
98 | Han, S. J., H. N. Chang, and J. Lee. 2001. Characterization of an oxygen-dependent inducible promoter, the nar promoter of Escherichia coli, to utilize in metabolic engineering. Biotechnol. Bioeng. 72: 573-576 |
99 | Stahl, S. and P. A. Nygren. 1997. The use of gene fusions to protein A and protein G in immunology and biotechnology. Pathol. Biol. (Paris) 45: 66-76 |
100 | Sauvonnet, N., I. Poquet, and A. P. Pugsley. 1995. Extracellular secretion of pullulanase is unaffected by minor sequence changes but is usually prevented by adding reporter proteins to its N- or C-terminal end. J. Bacteriol. 177: 5238-5246 |
101 | Tsuji, A., H. Koshimoto, Y. Sato, M. Hirano, Y. Sei-Iida, S. Kondo, and K. Ishibashi. 2000. Direct observation of specific messenger RNA in a single living cell under a fluorescence microscope. Biophys. J. 78: 3260-3274 |
102 | Honey, S., B. L. Schneider, D. M. Schieltz, J. R. Yates, and B. Futcher. 2001. A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex. Nucleic Acids Res. 29: E24 |
103 | Dong, H., L. Nilsson, and C. G. Kurland. 1996. Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates. J. Mol. Biol. 260: 649-663 DOI ScienceOn |
104 | Hamilton, S. R., J. B. O’Donnell, Jr., A. Hammet, D. Stapleton, S. A. Habinowski, A. R. Means, B. E. Kemp, and L. A. Witters. 2002. AMP-activated protein kinase kinase: Detection with recombinant AMPK alpha1 subunit. Biochem. Biophys. Res. Commun. 293: 892-898 |
105 | Jeong, K. J. and S. Y. Lee. 2002. Excretion of human betaendorphin into culture medium by using outer membrane protein F as a fusion partner in recombinant Escherichia coli. Appl. Environ. Microbiol. 68: 4979-4985 |
106 | Tunner, J. R. and C. R. Robertson. 1992. Use of glucose starvation to limit growth and induce protein production in Escherichia coli. Biotechnol. Bioeng. 40: 271-279 |
107 | Jonasson, P., J. Nilsson, E. Samuelsson, T. Moks, S. Stahl, and M. Uhlen. 1996. Single-step trypsin cleavage of a fusion protein to obtain human insulin and its C peptide. Eur. J. Biochem. 236: 656-661 |
108 | Koster, M., W. Bitter, and J. Tommassen. 2000. Protein secretion mechanisms in Gram-negative bacteria. Int. J. Med. Microbiol. 290: 325-331 |
109 | McKern, N. M., M. Lou, M. J. Frenkel, A. Verkuylen, J. D. Bentley, G. O. Lovrecz, N. Ivancic, T. C. Elleman, T. P. Garrett, L. J. Cosgrove, and C. W. Ward. 1997. Crystallization of the first three domains of the human insulin-like growth factor-1 receptor. Protein Sci. 6: 2663- 2666 |
110 | Hernan, R. A., H. L. Hui, M. E. Andracki, R. W. Noble, S. G. Sligar, J. A. Walder, and R. Y. Walder. 1992. Human hemoglobin expression in Escherichia coli: Importance of optimal codon usage. Biochem. 31: 8619-8628 |
111 | Middelberg, A. 2002. Preparative protein refolding. Trends Biotechnol. 20: 437 |
112 | Thomas, J. G. and F. Baneyx. 1997. Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli. Protein Expr. Purif. 11: 289-296 |
113 | Nishihara, K., M. Kanemori, M. Kitagawa, H. Yanagi, and T. Yura. 1998. Chaperone coexpression plasmids: Differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli. Appl. Environ. Microbiol. 64: 1694-1699 |
114 | Rosenberg, H. F. 1998. Isolation of recombinant secretory proteins by limited induction and quantitative harvest. Biotechniques 24: 188-191 PUBMED |
115 | Smolke, C. D. and J. D. Keasling. 2002. Effect of copy number and mRNA processing and stabilization on transcript and protein levels from an engineered dual-gene operon. Biotechnol. Bioeng. 78: 412-424 |
116 | Binet, R., S. Letoffe, J. M. Ghigo, P. Delepelaire, and C. Wandersman. 1997. Protein secretion by Gram-negative bacterial ABC exporters - a review. Gene 192: 7-11 |
117 | Brendel, V., P. Bucher, I. R. Nourbakhsh, B. E. Blaisdell, and S. Karlin. 1992. Methods and algorithms for statistical analysis of protein sequences. Proc. Natl. Acad. Sci. USA 89: 2002-2006 |
118 | Huang, H. C., M. Y. Sherman, O. Kandror, and A. L. Goldberg. 2001. The molecular chaperone DnaJ is required for the degradation of a soluble abnormal protein in Escherichia coli. J. Biol. Chem. 276: 3920-3928 |
119 | Puri, N., K. B. Appa Rao, S. Menon, A. K. Panda, G. Tiwari, L. C. Garg, and S. M. Totey. 1999. Effect of the codon following the ATG start site on the expression of ovine growth hormone in Escherichia coli. Protein Expr. Purif. 17: 215-223 |
120 | Taguchi, S., K. I. Nishihama, K. Igi, K. Ito, H. Taira, M. Motoki, and H. Momose. 2000. Substrate specificity analysis of microbial transglutaminase using proteinaceous protease inhibitors as natural model substrates. J. Biochem. (Tokyo) 128: 415-425 |
121 | Summers, R. G. and J. R. Knowles. 1989. Illicit secretion of a cytoplasmic protein into the periplasm of Escherichia coli requires a signal peptide plus a portion of the cognate secreted protein. Demarcation of the critical region of the mature protein. J. Biol. Chem. 264: 20074-20081 |
122 | Cornelis, P. 2000. Expressing genes in different Escherichia coli compartments. Curr. Opin. Biotechnol. 11: 450-454 |
123 | Hammarberg, B., P. A. Nygren, E. Holmgren, A. Elmblad, M. Tally, U. Hellman, T. Moks, and M. Uhlen. 1989. Dual affinity fusion approach and its use to express recombinant human insulin-like growth factor II. Proc. Natl. Acad. Sci. USA 86: 4367-4371 |
124 | Loo, T., M. L. Patchett, G. E. Norris, and J. S. Lott. 2002. Using secretion to solve a solubility problem: High-yield expression in Escherichia coli and purification of the bacterial glycoamidase PNGase F. Protein Expr. Purif. 24: 90-98 |
125 | Baneyx, F. and G. Georgiou. 1991. Construction and characterization of Escherichia coli strains deficient in multiple secreted proteases: Protease III degrades highmolecular- weight substrates in vivo. J. Bacteriol. 173: 2696-2703 |
126 | Kim, B. G. 1990. Analysis of pBR322 replication kinetics and its dependency on growth rate. Biotechnol. Bioeng. 36: 233-242 DOI PUBMED |
127 | Mergulhão, F., G. Monteiro, G. Larsson, A. Sandem, A. Farewell, T. Nystrom, J. Cabral, and M. Taipa. 2003. Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB. Appl. Microbiol. Biotechnol. 61: 495-501 |
128 | Moks, T., L. Abrahmsen, E. Holmgren, M. Bilich, A. Olsson, M. Uhlen, G. Pohl, C. Sterky, H. Hultberg, S. Josephson, et al. 1987. Expression of human insulin-like growth factor I in bacteria: use of optimized gene fusion vectors to facilitate protein purification. Biochem. 26: 5239-5244 |
129 | Rosenbaum, V., T. Klahn, U. Lundberg, E. Holmgren, A. von Gabain, and D. Riesner. 1993. Co-existing structures of an mRNA stability determinant. The 5' region of the Escherichia coli and Serratia marcescens ompA mRNA. J. Mol. Biol. 229: 656-670 |
130 | Ross, W., S. E. Aiyar, J. Salomon, and R. L. Gourse. 1998. Escherichia coli promoters with UP elements of different strengths: Modular structure of bacterial promoters. J. Bacteriol. 180: 5375-5383 |
131 | Workman, C. and A. Krogh. 1999. No evidence that mRNAs have lower folding free energies than random sequences with the same dinucleotide distribution. Nucleic Acids Res. 27: 4816-4822 |
132 | Netzer, W. J. and F. U. Hartl. 1998. Protein folding in the cytosol: Chaperonin-dependent and -independent mechanisms. Trends Biochem. Sci. 23: 68-73 |
133 | Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10: 1-6 |
134 | Prinz, W. A., F. Aslund, A. Holmgren, and J. Beckwith. 1997. The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm. J. Biol. Chem. 272: 15661-15667 |
135 | Baneyx, F. 1999. Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 10: 411-421 |
136 | Chen, G. F. and M. Inouye. 1990. Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes. Nucleic Acids Res. 18: 1465- 1473 |
137 | Grosjean, H. and W. Fiers. 1982. Preferential codon usage in prokaryotic genes: The optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene 18: 199-209 |
138 | Mergulhão, F. J. M., G. A. Monteiro, G. Larsson, M. Bostrom, A. Farewell, T. Nystrom, J. M. S. Cabral, and M. A. Taipa. 2003. Evaluation of inducible promoters on the secretion of a ZZ-Proinsulin fusion protein. Biotechnol. Appl. Biochem. 38: 87-93 |
139 | Schmidt, M., E. Viaplana, F. Hoffmann, S. Marten, A. Villaverde, and U. Rinas. 1999. Secretion-dependent proteolysis of heterologous protein by recombinant Escherichia coli is connected to an increased activity of the energy-generating dissimilatory pathway. Biotechnol. Bioeng. 66: 61-67 |
140 | Spanjaard, R. A. and J. van Duin. 1988. Translation of the sequence AGG-AGG yields 50% ribosomal frameshift. Proc. Natl. Acad. Sci. USA 85: 7967-7971 DOI ScienceOn |
141 | Kweon, D. H., D. H. Lee, N. S. Han, C. S. Rha, and J. H. Seo. 2002. Characterization of polycationic amino acids fusion systems for ion-exchange purification of cyclodextrin glycosyltransferase from recombinant Escherichia coli. Biotechnol. Prog. 18: 303-308 |
142 | Carrier, T., K. L. Jones, and J. D. Keasling. 1998. mRNA stability and plasmid copy number effects on gene expression from an inducible promoter system. Biotechnol. Bioeng. 59: 666-672 |
143 | Dias, A. 1990. Overproduction of proteins in Escherichia coli: Vectors, hosts and strategies. Methods Enzymol. 182: 93-112 DOI PUBMED |
144 | Estrem, S. T., T. Gaal, W. Ross, and R. L. Gourse. 1998. Identification of an UP element consensus sequence for bacterial promoters. Proc. Natl. Acad. Sci. USA 95: 9761-9766 |
145 | Lee, S. B. and J. E. Bailey. 2000. Analysis of growth rate effects on productivity of recombinant Escherichia coli populations using molecular mechanism models. Biotechnol. Bioeng. 26: 66-73 |
146 | Serra, E. C., N. Carrillo, A. R. Krapp, and E. A. Ceccarelli. 1993. One-step purification of plant ferredoxin-NADP+ oxidoreductase expressed in Escherichia coli as fusion with glutathione S-transferase. Protein Expr. Purif. 4: 539-546 |
147 | Terpe, K. 2003. Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60: 523-533 |
148 | Belagaje, R. M., S. G. Reams, S. C. Ly, and W. F. Prouty. 1997. Increased production of low molecular weight recombinant proteins in Escherichia coli. Protein Sci. 6: 1953-1962 |
149 | Chenal, A., P. Nizard, V. Forge, M. Pugniere, M. O. Roy, J. C. Mani, F. Guillain, and D. Gillet. 2002. Does fusion of domains from unrelated proteins affect their folding pathways and the structural changes involved in their function? A case study with the diphtheria toxin T domain. Protein Eng. 15: 383-391 |
150 | Goldman, E., A. H. Rosenberg, G. Zubay, and F. W. Studier. 1995. Consecutive low-usage leucine codons block translation only when near the 5 end of a message in Escherichia coli. J. Mol. Biol. 245: 467-473 |
151 | Levine, A. D., S. H. Rangwala, N. A. Horn, M. A. Peel, B. K. Matthews, R. M. Leimgruber, J. A. Manning, B. F. Bishop, and P. O. Olins. 1995. High level expression and refolding of mouse interleukin 4 synthesized in Escherichia coli. J. Biol. Chem. 270: 7445-7452 |
152 | Mackin, R. B. 1999. Streamlined procedure for the production of normal and altered versions of recombinant human proinsulin. Protein Expr. Purif. 15: 308-313 DOI PUBMED ScienceOn |
153 | Hellebust, H., M. Murby, L. Abrahmsen, M. Uhlen, and S. Enfors. 1989. Different approaches to stabilize a recombinant fusion protein. Bio/Technology 7: 165-168 |
154 | Kang, Y. and J. W. Yoon. 1994. Effect of modification of connecting peptide of proinsulin on its export. J. Biotechnol. 36: 45-54 |
155 | Lee, J., M. Cho, E. Hong, K. Kim, and J. Lee. 1996. Characterization of the nar promoter to use as an inducible promoter. Biotechnol. Lett. 18: 129-134 |
156 | Nakamura, Y. and K. Ito. 2002. A tripeptide discriminator for stop codon recognition. FEBS Lett. 514: 30-33 |
157 | Seffens, W. and D. Digby. 1999. mRNAs have greater negative folding free energies than shuffled or codon choice randomized sequences. Nucleic Acids Res. 27: 1578-1584 |
158 | Prytz, I., A. M. Sandén, T. Nystrom, A. Farewell, A. Wahlstrom, C. Forberg, I. Tubulekas, Z. Pragai, M. Barer, C. Harwood, and G. Larsson. 2002. Fed-batch production of recombinant beta-galactosidase using the universal stress promoters uspA and uspB in high cell density cultivation. Biotechnol. Bioeng. 83: 595-603 |
159 | Sawers, G. and M. Jarsch. 1996. Alternative regulation principles for the production of recombinant proteins in Escherichia coli. Appl. Microbiol. Biotechnol. 46: 1-9 |
160 | Uhlen, M., T. Moks, and L. Abrahmsen. 1988. Protein engineering to optimize recombinant protein purification. Biochem. Soc. Trans. 16: 111-112 |
161 | Makrides, S. C. 1996. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60: 512-538 PUBMED |
162 | Makoff, A. J., M. D. Oxer, M. A. Romanos, N. F. Fairweather, and S. Ballantine. 1989. Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons. Nucleic Acids Res. 17: 10191- 10202 |
163 | Fernandez, L. A. and V. de Lorenzo. 2001. Formation of disulphide bonds during secretion of proteins through the periplasmic-independent type I pathway. Mol. Microbiol. 40: 332-346 DOI ScienceOn |
164 | Kim, Y. S., H. C. Jung, and J. G. Pan. 2000. Bacterial cell surface display of an enzyme library for selective screening of improved cellulase variants. Appl. Environ. Microbiol. 66: 788-793 |
165 | Kitagawa, J., K. Yamamoto, and H. Iba. 2001. Computational analysis of sos response in ultraviolet-irradiated Escherichia coli. Genome Inf. 12: 280-281 |
166 | Mikhaleva, N. I., V. V. Golovastov, S. N. Zolov, M. V. Bogdanov, W. Dowhan, and M. A. Nesmeyanova. 2001. Depletion of phosphatidylethanolamine affects secretion of Escherichia coli alkaline phosphatase and its transcriptional expression. FEBS Lett. 493: 85-90 |
167 | Vasina, J. A. and F. Baneyx. 1997. Expression of aggregation-prone recombinant proteins at low temperatures: A comparative study of the Escherichia coli cspA and tac promoter systems. Protein Expr. Purif. 9: 211-218 |
168 | Stader, J. A. and T. J. Silhavy. 1990. Engineering Escherichia coli to secrete heterologous gene products. Methods Enzymol. 185: 166-187 |
169 | Stahl, S., J. Nilsson, S. Hober, M. Uhlen, and P. Nygren. 1999. Affinity fusions, gene expression, pp. 49-63. In M. Flickinger and Drew S. (eds.), Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. John Wiley & Sons, New York, U.S.A |
170 | Szweda, P., R. Pladzyk, R. Kotlowski, and J. Kur. 2001. Cloning, expression, and purification of the Staphylococcus simulans lysostaphin using the intein-chitin-binding domain (CBD) system. Protein Expr. Purif. 22: 467-471 |
171 | Zahn, K. 1996. Overexpression of an mRNA dependent on rare codons inhibits protein synthesis and cell growth. J. Bacteriol. 178: 2926-2933 |
172 | Herman-Antosiewicz, A., M. Obuchowski, and G. Wegrzyn. 2001. A plasmid cloning vector with precisely regulatable copy number in Escherichia coli. Mol. Biotechnol. 17: 193- 199 |
173 | Choe, W. S., R. H. Clemmitt, H. A. Chase, and A. P. Middelberg. 2002. Comparison of histidine-tag capture chemistries for purification following chemical extraction. J. Chromatogr. A. 953: 111-121 |
174 | Gottesman, M. E. and W. A. Hendrickson. 2000. Protein folding and unfolding by Escherichia coli chaperones and chaperonins. Curr. Opin. Microbiol. 3: 197-202 |
175 | Guisez, Y., I. Fache, L. A. Campfield, F. J. Smith, A. Farid, G. Plaetinck, J. Van der Heyden, J. Tavernier, W. Fiers, P. Burn, and R. Devos. 1998. Efficient secretion of biologically active recombinant OB protein (leptin) in Escherichia coli, purification from the periplasm and characterization. Protein Expr. Purif. 12: 249-258 |
176 | Stahl, S. and M. Uhlen. 1997. Bacterial surface display: Trends and progress. Trends Biotechnol. 15: 185-192 |
177 | Talmadge, K. and W. Gilbert. 1982. Cellular location affects protein stability in Escherichia coli. Proc. Natl. Acad. Sci. USA 79: 1830-1833 |
178 | Su, X., A. K. Prestwood, and R. A. McGraw. 1992. Production of recombinant porcine tumor necrosis factor alpha in a novel E. coli expression system. Biotechniques 13: 756-762 |
179 | Bothmann, H. and A. Pluckthun. 2000. The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines. J. Biol. Chem. 275: 17100-17105 |
180 | Clark, E. D. 2001. Protein refolding for industrial processes. Curr. Opin. Biotechnol. 12: 202-207 DOI PUBMED ScienceOn |
181 | Meerman, H. J. and G. Georgiou. 1994. Construction and characterization of a set of E. coli strains deficient in all known loci affecting the proteolytic stability of secreted recombinant proteins. Biotechnol. (N Y). 12: 1107-1110 |
182 | Mellies, J., R. Brems, and M. Villarejo. 1994. The Escherichia coli proU promoter element and its contribution to osmotically signaled transcription activation. J. Bacteriol. 176: 3638-3645 |
183 | Mergulhão, F. J., G. A. Monteiro, J. M. Cabral, and M. A. Taipa. 2001. A quantitative ELISA for monitoring the secretion of ZZ-fusion proteins using SpA domain as immunodetection reporter system. Mol. Biotechnol. 19: 239-244 |
184 | Zavialov, A. V., N. V. Batchikova, T. Korpela, L. E. Petrovskaya, V. G. Korobko, J. Kersley, S. MacIntyre, and V. P. Zav’yalov. 2001. Secretion of recombinant proteins via the chaperone/usher pathway in Escherichia coli. Appl. Environ. Microbiol. 67: 1805-1814 |
185 | Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57: 50- 108 PUBMED |
186 | Schenborn, E. and D. Groskreutz. 1999. Reporter gene vectors and assays. Mol. Biotechnol. 13: 29-44 |
187 | Shokri, A., A. M. Sandén, and G. Larsson. 2003. Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli. Appl. Microbiol. Biotechnol. 60: 654-664 |
188 | Tomme, P., A. Boraston, B. McLean, J. Kormos, A. L. Creagh, K. Sturch, N. R. Gilkes, C. A. Haynes, R. A. Warren, and D. G. Kilburn. 1998. Characterization and affinity applications of cellulose-binding domains. J. Chromatogr. B. Biomed. Sci. Appl. 715: 283-296 |
189 | Schaffner, J., J. Winter, R. Rudolph, and E. Schwarz. 2001. Cosecretion of chaperones and low-molecular-size medium additives increases the yield of recombinant disulfidebridged proteins. Appl. Environ. Microbiol. 67: 3994- 4000 |
190 | Stacey, S. N., D. Jordan, A. J. Williamson, M. Brown, J. H. Coote, and J. R. Arrand. 2000. Leaky scanning is the predominant mechanism for translation of human papillomavirus type 16 E7 oncoprotein from E6/E7 bicistronic mRNA. J. Virol. 74: 7284-7297 |
191 | Vasina, J. A., M. S. Peterson, and F. Baneyx. 1998. Scaleup and optimization of the low-temperature inducible cspA promoter system. Biotechnol. Prog. 14: 714-721 |
192 | Zhang, Y., D. R. Olsen, K. B. Nguyen, P. S. Olson, E. T. Rhodes, and D. Mascarenhas. 1998. Expression of eukaryotic proteins in soluble form in Escherichia coli. Protein Expr. Purif. 12: 159-165 |
193 | Misoka, F., T. Miyake, K. Miyoshi, M. Sugiyama, S. Sakamoto, and T. Fuwa. 1989. Overproduction of human insulin-like growth factor-II in Escherichia coli. Biotechnol. Lett. 11: 839-844 |
194 | Hasenwinkle, D., E. Jervis, O. Kops, C. Liu, G. Lesnicki, C. Haynes, and D. Kilburn. 1997. Very high-level production and export in Escherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system. Biotechnol. Bioeng. 55: 854-863 |
195 | Lai, W. B. and A. P. Middelberg. 2002. The production of human papillomavirus type 16 L1 vaccine product from Escherichia coli inclusion bodies. Bioprocess Biosyst. Eng. 25: 121-128 |
196 | Choi, J., K. Ra, and Y. Lee. 1999. Enhancement of bovine growth hormone gene expression by increasing the plasmid copy number. Biotechnol. Lett. 21: 1-5 |
197 | Guisez, Y., J. Robbens, E. Remaut, and W. Fiers. 1993. Folding of the MS2 coat protein in Escherichia coli is modulated by translational pauses resulting from mRNA secondary structure and codon usage: a hypothesis. J. Theor. Biol. 162: 243-252 |
198 | Lellouch, A. C. and R. A. Geremia. 1999. Expression and study of recombinant ExoM, a beta1-4 glucosyltransferase involved in succinoglycan biosynthesis in Sinorhizobium meliloti. J. Bacteriol. 181: 1141-1148 |
199 | Yokoyama, S. 2003. Protein expression systems for structural genomics and proteomics. Curr. Opin. Chem. Biol. 7: 39-43 |
200 | Murby, M., E. Samuelsson, T. N. Nguyen, L. Mignard, U. Power, H. Binz, M. Uhlen, and S. Stahl. 1995. Hydrophobicity engineering to increase solubility and stability of a recombinant protein from respiratory syncytial virus. Eur. J. Biochem. 230: 38-44 |