KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Implantation of Fetal Hepatocytes on Biodegradable Polymer Scaffolds

생분해성 고분자 담체를 이용한 태아 간세포의 이식

  • 곽소정 (한양대학교 공과대학 응용화학공학부) ;
  • 최동호 (순천향대학교 의과대학 외과학교) ;
  • 백승삼 (한양대학교 의과대학 병리학교) ;
  • 김상수 (서울대학교 생물화학공학 협동과) ;
  • 최차용 (서울대학교 생물화학공학 협동과) ;
  • 김병수 (한양대학교 공과대학 응용화학공학부)
  • Published : 2004.06.01
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Abstract

Whole liver transplantation, the currently available treatment of end-stage liver disease, has limitations including serious donor shortage, fatal surgical complications, risk of allograft rejection, and the requirement of life-long immunosuppression. In this study, we investigated the possibility of reconstructing liver tissues in vivo by implanting fetal hepatocytes on polymer scaffolds as a potential method to replace the current treatments. Fetal hepatocytes were freshly isolated from mice and seeded onto porous mesh scaffolds fabricated from polyglycolic acid, a biodegradable synthetic polymer. The seeded scaffolds were implanted into peritoneal cavity of athymic mice for one week. As a control, fetal hepatocytes were implanted without scaffold. One week after transplantation, liver-like tissues formed. Histological and immunohistochemical analyses indicated that the hepatocyles and liver tissue structures (bile ducts) were present in the newly formed tissues. In the control group, no transplanted hepatocytes were observed. Theses preliminary results suggest that liver tissues may be regeneration by transplanting fetal hepatocytes on polymer scaffolds.

본 연구에서는 생분해성 고분자 담체인 PGA 담체에 부착된 간세포의 이식을 통해서 이식된 간세포가 괴사하지 않고 남아 있으며 간 조직 구조의 일종인 담세관 유사구조를 확인하였다. 조직공학적인 간세포 이식 방법의 개발은 간 질환에 새로운 치료방법 개발의 가능성을 열어줄 수 있다.

Keywords

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