Browse > Article

Implantation of Fetal Hepatocytes on Biodegradable Polymer Scaffolds  

곽소정 (한양대학교 공과대학 응용화학공학부)
최동호 (순천향대학교 의과대학 외과학교)
백승삼 (한양대학교 의과대학 병리학교)
김상수 (서울대학교 생물화학공학 협동과)
최차용 (서울대학교 생물화학공학 협동과)
김병수 (한양대학교 공과대학 응용화학공학부)
Publication Information
KSBB Journal / v.19, no.3, 2004 , pp. 210-214 More about this Journal
Abstract
Whole liver transplantation, the currently available treatment of end-stage liver disease, has limitations including serious donor shortage, fatal surgical complications, risk of allograft rejection, and the requirement of life-long immunosuppression. In this study, we investigated the possibility of reconstructing liver tissues in vivo by implanting fetal hepatocytes on polymer scaffolds as a potential method to replace the current treatments. Fetal hepatocytes were freshly isolated from mice and seeded onto porous mesh scaffolds fabricated from polyglycolic acid, a biodegradable synthetic polymer. The seeded scaffolds were implanted into peritoneal cavity of athymic mice for one week. As a control, fetal hepatocytes were implanted without scaffold. One week after transplantation, liver-like tissues formed. Histological and immunohistochemical analyses indicated that the hepatocyles and liver tissue structures (bile ducts) were present in the newly formed tissues. In the control group, no transplanted hepatocytes were observed. Theses preliminary results suggest that liver tissues may be regeneration by transplanting fetal hepatocytes on polymer scaffolds.
Keywords
Fetal hepatocyte; polyglycolic acid; polymer scaffold; bile duct; liver;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Indications for orthotopic liver transplantation in fulminant liver failure /
[ Williams, R.;J. Wendon ] / Hepatology   DOI
2 Immunosuppression after liver transplantation /
[ Lucey, M. R. ] / J. Hepatology   DOI   ScienceOn
3 In situ split liver transplantation for two adult recipients /
[ Sommacale, D.;O. farges;G. M. Ettorre;P. Lebigot; A. Sauvanet; J. Marty;F. Durand;J. Belghiti ] / Transplantation
4 Local delivery of basic fibroblast growth factor increases both angiogenesis and engraftment of hepatocytes in tissue-engineered polymer devices /
[ Lee. H.; R. A. Cusick;F. Browne;T. H. Kim;P. X ma;H. Utsunomiya;R. Langer;J. P. Vacanti ] / Transplantation   DOI   ScienceOn
5 Hepatocyte transplantation using biodegradable matrices in ascorbic acid-deficient rats: comparison with heterotopically transplanted liver grafts /
[ Uyama, S.;P. M. Kaufmann;U. Kneser; H. C. Fiegel;J. M. Pollok;D. Kluth;J. P Vacanti;X. Rogiers ] / Transplantation   DOI   ScienceOn
6 Mouse hepatocytes migrate to liver parenchyma and function indefinitely after intrasplenic transplantation /
[ Ponder, K. P.;S. Gupta; F. Leland;G. Darlington;M. Finegold;J. DeMayo;F. D. Ledley;J. R. Chowdhury; S. L. Woo ] / Proc. Natl. Acad. Sci. USA
7 High yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study /
[ Berry, M. N. ] / J. Cell. Biol.   DOI   ScienceOn
8 Transfer of bilirubin uridine diphosphate-glucuronyltransferase to enzyme deficient rats /
[ Rugstad, H. E.;S. H. Robinson;C. Yannoni;H. Armen ] / Science   DOI
9 Intrahepatic hepatocyte transplantation following subtotal hepatectomy in the recipient: A possible medel in the treatment of hepatic enzyme deficiency /
[ Zhang, H.;E. Miescher-Clemens;G. Drugas;S. M. Lee;P. Colombani ] / J. Pediatr. Surg.   DOI   ScienceOn
10 The growth of transplanted liver cells within the pancreas /
[ Jaffe, V.;H. Darby;C. Selden;H. J. F. Hodgson ] / Tramsplantation   DOI   ScienceOn
11 Transplantation of isolated hepatocytes into the pancreas /
[ Vroemen, J. P.;W. A. Buurman;C. J. van der Linden;R. Visser;K. P. Heirwegh;G. Kootstra ] / Eur. Surg. Res.
12 Trophic factors from pancreatic islets in combined hepatocyte-islet allografts enhance hepatocellular survival /
[ Ricordi, C.;P. E. Lacy;M. P. Callery;P. W. Park;M. W. Flye ] / Surgery
13 Effects of partial hepatectomy on transplanted hepatocytes /
[ Jirtle, R. L.;G. Michalopoulos ] / Cnacer Res.
14 Selective cell transplantation using bioabsorbable artificial polymers as matrices /
[ Vacanti, J. P.;M. A. Morse;W. M. Saltzman;A. J. Domb;A. Perez-Atayde;R. Langer ] / J. Pediatr. Surg.   DOI   ScienceOn
15 Human fetal tissue research: practice, prospects, and policy /
[ Fine, A. ] / Cell Transplant.   DOI
16 Diversity of hepatic stem cells in the fetal and adult liver /
[ Zheng, Y. W.;H. Taniguchi ] / Semin. Liver Dis.   DOI   ScienceOn
17 Hepatocellular transplantation in acute liver failure /
[ Sutherland, D. E.;M. Numata;A. J. Matas;R. L. Simmons;J. S. Najarian ] / Surgery
18 Mouse hepatocytes migrate to liver parenchyma and function indefinitely after intrasplenic transplantation /
[ Ponder, K. P.;S. Gupta; F. Leland;G. Darlington;M. Finegold;J. DeMayo;F. D. Ledley;J. R. Chowdhury; S. L. C. Woo ] / Proc. Natl. Acad. Sci. USA   DOI   ScienceOn
19 Permanant engraftment and function of hepatocytes delivered to the liver: implications for gene therapy and liver regeneration /
[ Gupta, S.;E. Aragona;R. P. Vemuru;K. K. Bharava;R. D. Burk;J. R. Chowdhury ] / Hepatology   DOI
20 Hepatocellular transplantation for metabolic deficiencies: Decrease of plasma bilirubin in Gunn rats /
[ Matas, A. J.;D. E. Sutherland;M. W. Steffes;S. M. Mauer;A. Lowe;R. L. Simmons;J. S. Najarian ] / Science   DOI
21 Hepatocellular transplantation for treatment of D-galactosamine-induced acute liver failure in rats /
[ Sommer, B. G.;D. E. Sutherland;A. J. Matas;R. L. Simmons;J. S. Najarian ] / Transplant. Proc.
22 Selective intraportal hepatocyte transplantation in analbuminemic and Gunn rats /
[ Holzman, M. D.;J. Rozga;D. F. Neuzil;D. Griffin;A. D. Moscioni;A. A. Demetriou ] / Transplantation   DOI   ScienceOn
23 Enhancement of proliferation of intrasplenically transplanted hepatocytes in cirrhotic rats by hepatic stimulatory substance /
[ Jiang, B.;M. Sawa;T. Yamamoto; S. Kasai ] / Transplantation   DOI   ScienceOn
24 Observations on the fine structure of long-survived isolated hepatocytes inoculated into rat spleen /
[ Kusano, M.;M. Mito ] / Gastroenterology
25 Hepatocellular transplantation: Morphological study in hepatocytes transplanted into rat spleen /
[ Mito, M.;M. Kusano;T. Onishi;T. Saito;H. Ebata ] / Gastroenterol. Jpn.
26 Allogeneic and Xenogeneic hepatocyte transplantation /
[ Makowka, L.;L. E. Rotstein;R. E. Falk;J. A. Falk;R. Zuk;B. Langer;L. M. Blendis;M. J. Philips ] / Transplant. Proc.
27 Intrahepatic hepatocyte transplantation following subtotal hepatectomy in the recipient : A possible model in the treatment of hepatic enzyme deficiency /
[ Zhang, H.;E. M. Clemens;G. Drugas;S. M. Lee; P. Colombani ] / J. Pediatr. Surg.   DOI   ScienceOn
28 Delivery of whole liver-equivalent hepatocyte mass using polymer devices and hepatotrophic stimulation /
[ Uyama, S.;P. M. Kaufmann;T. Takeda;J. P. Vacanti ] / Tramsplantation   DOI   ScienceOn
29 The mesentery as a laminated vascular bed for hepatocyte transplantation /
[ Johnson, L. B.;J. Aiken;D. Mooney;B. L. Schoo;L. Griffith-Cima;R. Langer;J. P. Vacanti ] / Cell Transplant.   DOI
30 Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes /
[ Demetriou, A. A.;J. F. Whiting; D. Feldman;S. M .Levenson;N. R. Chowdhury;A. D. Moscioni;M. Kram;J. R. Chowdhury ] / Science   DOI