Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of the Crude Polysaccharide of Pleurotus eryngii on the Activation of Immune Cells

큰느타리버섯(Pleurotus eryngii) 조다당체의 면역세포 활성화 효과

  • Published : 2004.08.01
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Abstract

The objective of the current study was to determine the effects of the crude polysaccharide isolated from fruit body of Pleurotus eryngii on mouse splenocytes, B cells, and macrophages in vitro. The crude polysaccharides directly induced the proliferation of spleen cells in a dose-dependent manner and increased IL-6 and IFN-${\gamma}$ synthesis. The crude polysaccharides also increased the proliferation of B cells in a dose-dependent manner. The production of immunoglobulin Gl, G2a and IgG3 in the presence of the crude polysaccharides was increased progressively in the culture supernatant. When the crude polysaccharide were used in macrophage cell line (RA W264.7) stimulation, there were marked induction of NO synthesis in a dose-dependent manner and IL-6, TNF- r and GM-CSF synthesis. These results suggest that the crude polysaccharide isolated from fruit body of Pleurotus eryngii seem to act as a potent immunomodulator causing augmentation of immune cell activity, and thus could be used as a biological response modifier having possible therapeutic effects against immunological disorders, without any side effects.

큰느타리버섯의 기능성 식품으로서 활용도를 높이기 위해 동결 건조된 자실체에서 분리한 조다당체 추출물이 면역세포 활성에 미치는 효과를 조사한 결과는 다음과 같다. 조다당체 추출물은 300 및 1,000 $\mu$g/mL 농도에서 비장세포의 증식을 유도하였으며, 이 때 비장세포는 IL-6와 IFN-${\gamma}$ 분비를 유도하는 것으로 나타났다 조다당체 추출물은 농도 의존적으로B세포의 증식을 유도하였으며, 특히 100 $\mu$g/mL농도 이상에서는B세포의 증식이 현저히 증가하는 것으로 나타났다. 그리고 조다당체 추출물 1,000 $\mu$g/mL 농도에서 B세포가 생산하는 IgGl, IgG2a, IgG3의 분비량이 현저히 증가하였다. 또한 농도 의존적으로 대식세포주의 일산화질소 생산을 유도하였으며, 대식세포가 분비하는 IL-6, TNF-$\alpha$, GM-CSF의 생산도 현저히 증가하는 것을 확인할 수 있었다.

Keywords

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