Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Activity of Extract

삼백초(Saururus chinensis Baill) 추출물의 항균활성

  • 고무석 (전남대학교 사범대학 가정교육과)
  • Published : 2004.08.01
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Abstract

In order to develop natural food preservatives, the ethanol and water extracts of the Saururus chinensis Baill were prepared. Antimicrobial activity was examined against 10 kinds of harmful microorganisms. The ethanol and water extracts showed the most active antimicrobial activity against B. subtilis and E. coli. The ethanol extract showed stronger antimicrobial activity than that of the water extract. However, the extracts did not show any antimicrobial activity against lactic acid bacteria and yeast. The minimum inhibitory concentrations (MIC) of ethanol extracts against B. subtilis and E. coli were 5 to 10 mg/mL, respectively. Antimicrobial activity of the ethanol extract was not destroyed at 40-12$0^{\circ}C$ and pH 3∼11. The ethanol extract was fractionated in the order of hexane, diethyl ether, ethyl acetate, and water fractions. The highest antimicrobial activity was found in the diethyl ether fraction.

삼백초 잎과 줄기의 에탄올 추출물과 그의 분획물을 일반세균, 젖산균 및 효모 총 10 균주에 대한 항균활성을 검색하고, 항균활성 물질의 미생물에 대한 최소저해농도와 증식에 미치는 영향 및 열과 pH에 대한 안정성, 용매분획 후 각 분획별 항균활성을 조사한 결과는 다음과 같다. 삼백초 에탄올추출물과 물추출물에서 항균활성 이 가장 높게 나타난 것은 그람양성균인 B. subtilis와 그람음성균인 E. coli이었으며, 에탄올추출물의 경우에는 물추출물보다 항균활성은 모든 균주에서 대부분 더 높게 나타났다. 삼백초 에탄올추출물에 대한 그람양성균과 그람음성균의 최소저해농도는 5∼10 mg/mL으로 나타났으며, 젖산균과 효모는 20 mg/mL 이하의 농도에서는 항균활성이 나타나지 않았다. 에탄올추출물이 미생물 증식에 미치는 영향은 B. subtilis와 E. coli이 경우 5mg/mL 이상의 농도에서 균증식이 현저하게 억제되었다. 젖산균과 효모는 에탄올 추출물의 농도가 증가함에 따라 항균활성이 증가하였으나,1.5 mg/mL 이하의 저농도에서는 균증식이 8시간까지 억제되다가 그 후 시간이 경과함에 따라 현저하게 균증식이 증가하였다. 에탄올 추출물이 대수 증식기에 미치는 영향은 5. aureus, E. coil의 경우 대조구에 비하여 균증식이 억제되었으며 2.5 mg/mL 이상의 농도에서는 농도에 따른 차이는 거의 나타나지 않았다. 항균활성 물질의 열 및 pH 안정성은 40∼ 12$0^{\circ}C$ 및 pH 3∼11까지 모든 균주의 생육환의 크기가 대조구와 비슷하게 나타나 열과 pH에 안정한 것으로 확인되었다. 에탄올추출물을 용매 분획한 결과 에테르 분획물의 항균활성이 가장 높았고, 그람양성균과 그람음성균의 항균활성은 젖산균과 효모보다 더 높게 나타났다.

Keywords

References

  1. 박종희, 이정규. 2000. 상용약용식물도감. 도서출판 신일상사, 서울. p 202-203.
  2. Lee YN. 2002. Flora of Korea. Kyo-Hak Publishing Co., Ltd., Seoul. p 218-219.
  3. 권설안. 1999. 속 삼백초와 어성초에 대하여. 대한한약 2: 18-20.
  4. 권설안. 1999. 속 삼백초와 어성초에 대하여. 대한한약 3: 28-31.
  5. Choe KH. 1999. A study on chemical composition and antimicrobialactivity of Saururaceae growing in Korea. PhD Dissertation. Kyung Hee University, Korea.
  6. 한국약용식물학 연구회. 2001. 종합약용식물학. 학창사, 서울. p 176.
  7. Park HK. 2002. A study on the antimicrobial activity ofHouttuynia cordata Thunb. MS Thesis. Sunchon NationalUniversity, Korea.
  8. Choi YH, Kim EY, Park KY, Rhee SH, Lee WH. 1994.Antimutagenic effects of the juice and boiling water extractof Houttuynia cordata thunb. J Korean Soc Food Nutr 23:916-921.
  9. Kim KY, Chung DO, Chung HJ. 1997. Chemical compositionand antimicrobial activities of Houttuynia cordata thunb.Korean J Food Sci Technol 29: 400-406.
  10. Kong YJ, Park BK, Oh DH. 2001. Antimicrobial activity ofQuercus mongolica leaf ethanol extract and organic acidsagainst food-borne microorganisms. Korean J Food SciTechnol 33: 178-183.
  11. Kim YD, Kang SK, Choi OJ, Lee HC, Jang MJ, Shin SC. 2000. Screening of antimicrobial activity of Chopi (Zanthoxylum piperitum A.P. DC.) extract. J Korean Soc Food Sci Nutr 29: 1116-1122.
  12. Kim YD, Kang SK, Choi OJ. 2001. Antimicrobial activity of coriander (Coriandrum sativum L.) extract. J KoreanSoc Food Sci Nutr 30: 692-696.
  13. Kong YJ, Jong GP, Kwon HJ, Hong JK, Park BK, Oh DH.2001. Antimicrobial activities of Quercus spp. leaf ethanolextract against foodborne disease microorganism. J KoreanSoc Food Sci Nutr 30: 415-420.
  14. Oh DH, Lee MK, Park BK. 1999. Antimicrobial activites of commercially available tea on the harmful foodborne organism.J Korean Soc Food Sci Nutr 28: 100-106.
  15. Lee HY, Kim CK, Sung TK, Mun TK, Lim CJ. 1992. Antimicrobial activity of Ulmus pumila L. extract. Korean JAppl Microbial Biotechnol 20: 1-5.
  16. Han JS, Shin DH. 2001. Antimicrobial activity of Lysimachiaclethroides Duby extracts on food-borne microorganisms.Korean J Food Sci Technol 33: 774-783.
  17. Kim YD, Kim HK, Kim KJ. 2003. Antimicrobial activity of solvent fraction from Cornus officianalis. J Korean SocFood Sci Nutr 32: 829-832. https://doi.org/10.3746/jkfn.2003.32.6.829
  18. Cho DB. 2001. A study on the constituents and antimicrobial substance during the growth stage of Lentinus edodes sing.PhD Dissertation. Sunchon Nation University, Korea.
  19. Lee JY, Min YK, Kim HY. 2001. Isolation of antimicrobial substance from Schizandra chinensis Baillon and antimicrobialeffect. Korean J Food Sci Technol 33: 389-394.
  20. Kong YJ, Kang TS, Lee MK, Park BK, Oh DH. 2001. Antimicrobialand antioxidative activities of solvent fractionsof Quercus mongolica leaf. J Korean Soc Food Sci Nutr30: 338-343.
  21. Shin GJ. 1999. Synergistic effect of Panax ginseng C.A. Meyer, Saururus Chinensis (Lour.) Baill and Rubus coreanus Miq. extracts on antioxidative activity in rats. MS Thesis. Kangwon National University, Korea.
  22. Seong SH. 1998. Hepatoprotective lignans of Saururus Chinensis. PhD Dissertation. Seoul National University,Korea.
  23. Jin SW. 1998. Rhizoma Saururi (RS) induced the morphological change of the peritoneal macrophages through nitric oxide (NO). MS Thesis. Wonkwang University, Korea.
  24. Jeon GH. 1998. Studies on the mechanism of nitric oxide (NO) induction in the pertoneal macrophages by Herba Saurui (HS). PhD Dissertation. Wonkwang University,Korea.
  25. Kwak JW, Kwon CH. 1988. Pharmacological studies on Saururus chinensis Baill. Kyung Hee University Bulletinof Kyung Hee Pharmaceutical Science 16: 137-154.
  26. Kim BH, Song WS. 2000. The dyeability and antimicrobialactivity of Saururus chinensis (I). J Korean Home EconomicsAssoc 38: 1-9.
  27. Park SK, Oh GJ, Kim HT, Kim HJ, Chung SG, Cho EH.1998. Analgesic constituent from the herba of Saururus chinensis (Lour.) Baill. Yakhak Hoeji 42: 238-239.
  28. Park JH. 1998. Effects of seed tuber position and number of nodes on growth and yield of Saururus Chinensis Baill. MS Thesis. Chungbuk National University, Korea.
  29. Bauer AW, Kibby MM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk metheod. AM J Clin Pathol 45: 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  30. Piddock LJV. 1990. Techniques used for the determinationof antimicrobial resistance and sensitivity in bacteria. JAppl Bacteriol 68: 307-318. https://doi.org/10.1111/j.1365-2672.1990.tb02880.x
  31. Branch A, Starkey DH, Power EE. 1965. Diversificationsin the tube dilution test for antibiotic sensitivity of microorganisms.Appl Microbiol 13: 469-472.
  32. MacLowry JD, Jaqua MJ. 1970. Detailed methodology andimplementation semiautomated serial dilution microtechniquefor antimicrobial susceptibility testing. Appl Microbiol20: 46-53.
  33. EI-Shenawy MA, Marth EH. 1989. Behavior of Listeriamonocytogenes in the presence of sodium propionate. JFood Microbiol 8: 85-89. https://doi.org/10.1016/0168-1605(89)90084-6
  34. Nakamura S, Kato Am, Kobayashi K. 1991. New antimicrobialcharacteristics of lysozyme-dextran conjugate. JAgric Food chem 39: 647-650. https://doi.org/10.1021/jf00004a003

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