Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Maengjong-Juk Extract Coated Rice Supplementation on Antioxidative System in Rabbit Fed High Cholesterol Diet

맹종죽 추출물 코팅쌀 식이가 고콜레스테롤 섭취 토끼의 항산화 시스템에 미치는 영향

  • 이민자 (인제대학교 식품생명과학부, 바이오헬스소재연구센터, 식품과학연구소) ;
  • 김은영 (인제대학교 식품생명과학부, 바이오헬스소재연구센터, 식품과학연구) ;
  • 문갑순 (인제대학교 식품생명과학부, 바이오헬스소재연구센터, 식품과학연구소)
  • Published : 2004.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

To evaluate the antioxidative effect of rice coated with maengjong-juk extract in vivo system, rice coated with maengjong-juk extract diets were fed to NZW rabbit for 16 weeks and lipid peroxidation, protein oxidation, activities of antioxidative enzymes and total glutathione content in tissues were measured. TBARS contents in liver and spleen were significantly decreased in maengjong-juk extract diet group compared to control group, while those in kidney and heart tissue were not significantly different. Maengjong-juk extract diet suppressed the protein oxidation significantly in liver, spleen, kidney and heart tissues. Hepatic total SOD, Cu$.$Zn-SOD and Mn-SOD activities of maengjong-juk extract diets were significantly higher than those of control diet. GSH-Px and catalase activities of maengjong-juk extract diet were higher than those of control, while GR activities show no significant difference between the two groups. Total hepatic glutathione content was significantly increased by maengjong-juk extract diet. According to this study, many antioxidative materials and phytochemicals in maengjong-juk extracts seems to protect tissues from oxidative stress by stimulating antioxidative systems in atherosclerotic rabbit fed high cholesterol diet.

맹종죽 추출물 코팅쌀 식이가 동맥경화 유발 토끼의 항산화 및 항동맥경화 효과에 미치는 영향을 평가하기 위해 NZW계 토끼에게 고콜레스테롤 식이에 대나무 코팅쌀을 첨가한 식이를 16주간 급여하면서 항산화 시스템에 미치는 영향을 조사하였다. 간, 비장, 신장 및 심장 조직에서의 지질과산화 정도를 TBARS 값으로 측정한 결과 간과 비장에서는 대조군에 비해 맹종죽 코팅쌀 첨가식이군에서 지질과산화가 효과적으로 억제되었고 신장과 심장에서는 대조군에 비해 대나무 코팅쌀 첨가 식이군에서 낮았으나 유의적인 차이는 관찰되지 않았다. 단백질 카르보닐 함량에 있어서도 대조군에 비해 맹종죽 코팅쌀 첨가 식이군에서 현저히 낮은 값을 나타내어 맹종죽 코팅쌀 첨가 식이는 조직 단백질 산화를 상당히 억제하였다. 간에서의 항산화 효소계 활성을 측정한 결과 total SOD, Cu$.$Zn-SOD 및 Mn-SOD 활성은 맹종죽 코팅쌀 첨가 식이군에서의 활성이 대조군에 비해 현저하게 높았으며 GSH-Px, catalase 활성 또한 같은 경향을 나타내었다. GR 활성은 대조군에 비해 대나무 코팅쌀 첨가 식이군에서 높았으나 유의적이 차이는 관찰되지 않았다. 간 조직 내의 총 글루타치온 함량은 대조군에 비해 대나무 코팅쌀 첨가 식이군에서 유의적으로 높은 값을 나타내어 대조군보다 27% 정도 증가하였다. 따라서, 맹종죽 코팅쌀 내에 함유된 항산화 물질들에 의해 동맥 경화 유발 토끼의 항산화 시스템을 적극적으로 보호함을 확인하였다.

Keywords

References

  1. International Congress Series v.1229 Nutritional modification of cardiovascular disease risk Merete O https://doi.org/10.1016/S0531-5131(01)00463-0
  2. Am J Med v.113 Randomized clinical trials on the effects of dietary fat and carbohydrate on plasma lipoproteins and cardiovascular disease Sacks FM;Katan M https://doi.org/10.1016/S0002-9343(01)00987-1
  3. Am J Med v.113 Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer Kris Etherton PM;Hecker KD;Bonanome A;Coval SM;Binkoski AE;Hilpert KF;Griel AE;Etherton TD https://doi.org/10.1016/S0002-9343(01)00995-0
  4. Prev Med v.35 Dietary patterns and the odds of carotid atherosclerosis in women: The Framingham Nutrition Studies Millen BE;Quatromoni PA;Nam B.H.;O'Horo CE;Polak JF;D'Agostino RB https://doi.org/10.1006/pmed.2002.1116
  5. Nutrition Research v.20 The potential role of biotin insufficiency on essential fatty acid metabolism and cardiovascular disease risk Ho RC;Cordain L https://doi.org/10.1016/S0271-5317(00)00201-3
  6. Biomedicine & Pharmacotherapy v.57 Biochemical pharmacology of functional foods and prevention of chronic diseases of aging Ferrari C;Torres E https://doi.org/10.1016/S0753-3322(03)00032-5
  7. Nutrition v.16 Nutraceuticals and functional foods: introductior and meaning Hardy G https://doi.org/10.1016/S0899-9007(00)00332-4
  8. Nutrition v.20 Elucidating the role of nutraceuticals in overexpressing antiapoptotic proteins in prostate cancer Rafi MM https://doi.org/10.1016/j.nut.2003.09.014
  9. Experimental Gerontology v.35 Nutraceutical interventions may delay aging and the age-related diseases Villeponteau B;Cockrell R;Feng J https://doi.org/10.1016/S0531-5565(00)00182-0
  10. Phytochemistry v.60 Genistein Dixon RA;Ferreira D https://doi.org/10.1016/S0031-9422(02)00116-4
  11. Peptides v.24 New antihypertensive peptides isolated from rapeseed Marczak ED;Usui H;Fujita H;Yang Y;Yokoo M;Lipkowski AW;Yoshikawa M https://doi.org/10.1016/S0196-9781(03)00174-8
  12. J Food Sci v.55 Difference between bamboo shoots and vegetables in thermal disintegration fo tissues and polysaccharides fractionated by successive extraction Michiko F https://doi.org/10.1111/j.1365-2621.1990.tb05219.x
  13. J Agric Food Chem v.49 Identifiation and antioxidant activity of novel chlorogenic acid derivatives from bamboo (Phyllostachys edulis) Kweon M.;Hwang H.J.;Sung H.C. https://doi.org/10.1021/jf010514x
  14. J Agric Food Chem v.48 Evaluation of antioxidant and prooxidant activities of bamboo (Phyllostachys nigra var.) Henonis leaf extract in vitro Hu C;Zhang Y;Kitts DD https://doi.org/10.1021/jf0001637
  15. J Korean Soc Food Sci Nutr v.25 Physiological and antibacterial activity of bamboo (Sasa coreana Nakai) leaves Kim M.J.;Byun M.W.;Jang M.S.
  16. Korean J Postharvest Sci Technol v.8 Functional properties and antimicrobial activity of bamboo (Phyllostachys sp.) extracts Kim N.K.;Cho S.H.;Lee S.D.;Ryu J.S.;Shim K.H.
  17. Korean J Food Sci Technol v.34 Antimicrobial activities of ethanol extracts from Korean bamboo culms and leaves Baek J.W.;Jung S.H.;Moon G.S.
  18. J Food Sci Nutr v.1 Effect of bamboo (Pseudosasa japonica Makino) leaves on the quality and sensory characteristics of Dongchimi Kim M.J.;Kim B.K.;Jang M.S.
  19. Korean J Food Sci Technol v.27 Antimicrobial activity of bamboo leaves extract on microorganisms related to kimchi fermentation Chung D.K.;Yu R.N.
  20. Korean J Dietary Culture v.17 Effects of methanol extracts from bamboo (Pseudosasa japonica Makino) leaves extracts on lipid metabolism in rats fed high fat and high cholesterol diet Shin M.K.;Han S.H.
  21. Korean J Food Sci Technol v.35 Antioxidative effects of Korean bamboo trees, wang-dae, som-dae, maengjong-juk, jolitdae and o-juk Lee M.J.;Moon G.S.
  22. Atherosclerosis v.160 Is there a genetic basis for resistance to atherosclerosis? Stein O;Thiery J;Stein Y https://doi.org/10.1016/S0021-9150(01)00664-5
  23. Biomedicine & Pharmacotherapy v.56 Atherosclerosis and cancer: common pathways on the vascular endothelium Morganti M;Carpi A;Nicolini A;Gorini I;Glaviano B;Fini M;Giavaresi G;Mittermayer C;Giardino R https://doi.org/10.1016/S0753-3322(02)00242-1
  24. Cardiovascular Surgery v.9 Development of atherosclerosis and plaque biology Kadar A;Glasz T https://doi.org/10.1016/S0967-2109(00)00097-1
  25. Atherosclerosis v.141 Oxidant and antioxidants in the pathogenesis of atherosclerosis: implications for the oxidized low density lipoprotein hypothesis Heinecke JW
  26. The Research and Extension v.31 Breeding strategy for enhancing the utility of rice Choi H.C.
  27. Analytical Biochemistry v.95 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction Ohkawa H;Ohishi N;Yagi K https://doi.org/10.1016/0003-2697(79)90738-3
  28. J Biol Chem v.262 Age-related changes in oxidized proteins Oliver CN;Ahn BW;Moerman EJ;Goldstein S;Stadtman ER
  29. Methods in Enzymology v.186 Determination of carbonyl content in oxidatively modified proteins Livine RL;Garland D;Oliver CN;Amici A;Climent I;Lenz AG;Ahn BW;Shaltiel S;Stadtman ER https://doi.org/10.1016/0076-6879(90)86141-H
  30. Anal Biochem v.4 Revaluation of assay methods and establishment of kit for superoxide dismutase activity Oyanagui Y
  31. Biochem Biophys Res Commun v.71 Glutathione peroxidase activity in selenium-dificient rat liver Lawrence RA;Burk F https://doi.org/10.1016/0006-291X(76)90747-6
  32. Methods in Enzymology v.113 Glutathione reductase Inger C;Bengt M https://doi.org/10.1016/S0076-6879(85)13062-4
  33. Methods in Enzymology v.105 Catalase in vitro Aebei H https://doi.org/10.1016/S0076-6879(84)05016-3
  34. Anal Biochem v.27 Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: appli-cation to mammalian blood and other tissues Tietze F https://doi.org/10.1016/0003-2697(69)90064-5
  35. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities o protein utilizing the principle of protein-dye binding Bradford MM https://doi.org/10.1016/0003-2697(76)90527-3
  36. Free Rad Biol Med v.32 Oxidized amino acids: culprits in human atherosclerosis and indicators of oxidative stress Heinecke JW https://doi.org/10.1016/S0891-5849(02)00792-X
  37. Pharmacology & Therapeutics v.89 Oxidative pathways in cardioascular disease: roles, mechanisms, and therapeutic implications Wattanapitayakul SK;Bauer JA https://doi.org/10.1016/S0163-7258(00)00114-5
  38. Circulation v.91 Atherosclerosis: Basic mechanisms: Oxidation, inflammation, and genetics Berliner JA;Navab M;Fogelman AM;Frank JS;Demer LL;Edwards PA;Watson AD;Lusis AJ https://doi.org/10.1161/01.CIR.91.9.2488
  39. Atherosclerosis v.167 Autoantibodies against modified low-density lipoproteins in coronary artery disease Tornvall P;Waeg G;Nilsson J;Hamsten A;Regnstrom J https://doi.org/10.1016/S0021-9150(03)00021-2
  40. Clinica Chimica Acta v.317 Comparison of antioxidant effects of naringin and probocol in cholesterol -fed rabbirs Jeon S.M.;Bok S.H.;Jang M.K.;Kim Y.H.;Nam K.T.;Jeong T.S.;Park Y.B.;Choi M.S. https://doi.org/10.1016/S0009-8981(01)00778-1
  41. J Korean Soc Food Sci Nutr v.31 Effects of Korean wheat on LDL oxidation and atherosclerosis in cholesterol-fed rabbits Choe M.;Kim H.S. https://doi.org/10.3746/jkfn.2002.31.1.104
  42. Free Rad Biol Med v.31 Effect of vitamin E on aortic lipid oxidation and intimal proliferation after arterial injury in cholesterol-fed rabbits Upston JM;Witting PK;Brown AJ;Stocker R;Keaney JF https://doi.org/10.1016/S0891-5849(01)00721-3
  43. Atherosclerosis v.141 Dual effects of the antioxidant agents probucol and carvedilol on proliferative and fatty lesions in hypercholesterolemic rabbits Donetti E;Soma MR;Barberi L;Paoletti R;Fumagalli R;Roma P;Catapano AL https://doi.org/10.1016/S0021-9150(98)00146-4
  44. J Biol Chem v.262 Protein damaged by oxygen radicals are rapidly degraded in extracts of red blood cells Davies KJ;Goldberg AL
  45. FEBS Letters v.437 Generation of protein carbonyls by glycoxidation and lipoxidation reactions with autoxidation products of ascorbic acid and polyunsaturated fatty acids Miyata T;Inagi R;Asahi K;Yamada Y;Horie K;Sakai H;Uchida K;Kurokawa K https://doi.org/10.1016/S0014-5793(98)01079-5
  46. Free Rad Biol Med v.29 Antiatherogenic effect of coenzyme Q10 in apolipoprotein E gene knockout mice Witting PK;Pettersson K;Letters J;Stocker R https://doi.org/10.1016/S0891-5849(00)00311-7
  47. Life Sciences v.69 Antioxidant activity of naringin and lovastatin in high cholesterol-fed rabbits Jeon S.M.;Bok S.H.;Jang M.K.;Lee M.K.;Nam K.T.;Jeong T.S.;Park Y.B.;Rhee S.J.;Choi M.S. https://doi.org/10.1016/S0024-3205(01)01363-7
  48. Life Sciences v.58 Effects of probucol on hypercholesterolemia-induced changes in antioxidant enzymes Mantha SV;Kalra J;Prasad K https://doi.org/10.1016/0024-3205(95)02315-1
  49. Life Sciences v.74 Naringin alters the cholesterol biosynthesis and antioxidant enzyme activities in LDL receptor-knockout mice under cholesterol fed condition Kim H.J.;Oh G.T.;Park Y.B.;Lee M.K.;Seo H.J.;Choi M.S. https://doi.org/10.1016/j.lfs.2003.08.026
  50. Prostaglandins & Other Lipid Mediators v.66 Effects of fish oil and vitamin E on the antioxidant defense system in diet-induced hypercholesterolemic rabbits Hsu H.C.;Lee Y.T.;Chen M.F. https://doi.org/10.1016/S0090-6980(01)00146-0
  51. Food Chemistry v.83 Antioxidant action of flavonoids from Mangifera indica and Emblica officinalis in hypercholesterolemic rats Anila L;Vijayalakshmi NR https://doi.org/10.1016/S0308-8146(03)00155-9
  52. Nutrition v.18 Free radicals, antioxidants, and nutrition Fang Y.Z.;Yang S.;Wu G. https://doi.org/10.1016/S0899-9007(02)00916-4
  53. Free Rad Biol Med v.25 Chemical biology of nitric oxide: Insight into regulatory, cytotoxic, and cytoprotective mechanism of nitric oxide Wink DA;Mitchell JB https://doi.org/10.1016/S0891-5849(98)00092-6
  54. Cardiovascular Research v.53 Chronic antioxidant supplementation attenuates nuclear factor-B activation and preserves endothelial function in hypercholesterolemic pigs Rodriguez Porcel M;Lerman LO;Holmes DR;Richardson D;Napoli C;Lerman A https://doi.org/10.1016/S0008-6363(01)00535-1
  55. Clinica Chimica Acta v.328 Changes of oxidative stress in various tissues by long-term administration of vitamin E in hypercholesterolemic rats Cahide G;Tannaz M https://doi.org/10.1016/S0009-8981(02)00388-1
  56. Atherosclerosis v.163 Increased macrophage glutathione content reduces cell-mediated oxidation of LDL and atherosclerosis in apolipoprotein E -deficient mice Rosenblat M;Coleman R;Aviram M https://doi.org/10.1016/S0021-9150(01)00744-4
  57. Arch Med Res v.33 Decreased serum total antioxidant status and erythrocyte-reduced glutathione levels are associated with increased serum malondialdehyde in atherosclerotic patients Tamer L;Sucu N;Polat G;Ercan B;Barlas A;Yucebilgic G;Unlu A;Dikmengil M;Atik U https://doi.org/10.1016/S0188-4409(01)00381-2

Cited by

  1. Effects of grape pomace on the antioxidant defense system in diet-induced hypercholesterolemic rabbits vol.4, pp.2, 2010, https://doi.org/10.4162/nrp.2010.4.2.114