Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Characterization of Fetal Gonad-Derived Cells by Stem Cell Markers

줄기세포 Marker를 이용한 돼지 태아 생식선 유래 세포의 특성화

  • Choi, S. C. (School of Life Sciences and Biotechnology, Korea University) ;
  • H. H. Yeon (School of Life Sciences and Biotechnology, Korea University) ;
  • S. K. Choi (School of Life Sciences and Biotechnology, Korea University) ;
  • H. Lee (School of Life Sciences and Biotechnology, Korea University) ;
  • S. Hong (School of Life Sciences and Biotechnology, Korea University) ;
  • C. S. Park (Division of Animal Science ＆ Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • S. H. Lee (Kongju University) ;
  • S. H. Lee (School of Life Sciences and Biotechnology, Korea University)
  • Published : 2004.03.01
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Abstract

In mammals, male and female germline stem cells are derived from primodial germ cells. Despite many efforts to identify stem cells from gonads, there has been little successe to identify germline stem cells yet. In this study, we isolate and characterized porcine germline stem cells using only stem cell markers that are prevalently expressed in various tissues. Gonadal cells derived from both male and female formed colonies and showed AP activities and different lectin binding properties. Pluripotency of germline stem cells was also identified by positive signals against putative stem cells markers such as SSEA-1 and SSEA-3. In addition, nestin was also found in primary gonad cells that have a similar morphology to the AP-positive cells. The nestin expression suggests that the germline stem cells may have similar expression of the prevalent stem cell markers found in other tissues. The demonstration of nestin expression together with pluripotent cell markers calls further investigation of the possible differentiation of nestin-positive cells into neurons.

Keywords

References

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