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Characterization of Fetal Gonad-Derived Cells by Stem Cell Markers  

Choi, S. C. (School of Life Sciences and Biotechnology, Korea University)
H. H. Yeon (School of Life Sciences and Biotechnology, Korea University)
S. K. Choi (School of Life Sciences and Biotechnology, Korea University)
H. Lee (School of Life Sciences and Biotechnology, Korea University)
S. Hong (School of Life Sciences and Biotechnology, Korea University)
C. S. Park (Division of Animal Science ＆ Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
S. H. Lee (Kongju University)
S. H. Lee (School of Life Sciences and Biotechnology, Korea University)
Publication Information
Reproductive and Developmental Biology / v.28, no.1, 2004 , pp. 65-70 More about this Journal
Abstract
In mammals, male and female germline stem cells are derived from primodial germ cells. Despite many efforts to identify stem cells from gonads, there has been little successe to identify germline stem cells yet. In this study, we isolate and characterized porcine germline stem cells using only stem cell markers that are prevalently expressed in various tissues. Gonadal cells derived from both male and female formed colonies and showed AP activities and different lectin binding properties. Pluripotency of germline stem cells was also identified by positive signals against putative stem cells markers such as SSEA-1 and SSEA-3. In addition, nestin was also found in primary gonad cells that have a similar morphology to the AP-positive cells. The nestin expression suggests that the germline stem cells may have similar expression of the prevalent stem cell markers found in other tissues. The demonstration of nestin expression together with pluripotent cell markers calls further investigation of the possible differentiation of nestin-positive cells into neurons.
Keywords
Porcine; Germline stem cells; SSEA-1; SSEA-3; Nestin;
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