한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제21권1호
  • /
  • Pages.23-28
  • /
  • 2004
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개 말초혈액 탐식세포의 탐식반응에 대한 1,2-benzopyrone의 면역자극 효과

Immunostimulating Effect of 1,2-Benzopyrone on Phagocytic Response of Canine Peripheral Blood Phagocytes

  • 신정화 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 나기정 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 양만표 (충북대학교 수의과대학 및 동물의학연구소)
  • 발행 : 2004.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

1,2-benzopyrone has been shown to affect on the activation and stimulation of macrophage. To examine the immunostimulating effect of 1,2-benzopyrone on the phagocytic response of canine peripheral blood mononuclear cells (PBMC) as well as polymorphonuclear cells (PMN), the phagocytic activity of phagocytes was analyzed by flow cytometry system using FITC-labelled latex. The 1,2-benzopyrone did not show any direct effect on phagocytic response of PBMC and PMN. But it showed an enhanced effect on the phagocytic response of monocyte-rich cells fractioned by cell size from dot plot profile in flowcytometric cytography of PBMC. The phagocytic activity of these cells was also enhanced by addition of culture supernatant from PBMC treated with 1,2-benzopyrone. Similarly, the phagocytic activity of PMN but not PBMC in the same procedures was enhanced by culture supernatant from PBMC treated with 1,2-benzopyrone. However, the culture supernatant from PMN treated with 1.2-benzopyrone did not show the enhancing effect on phagocytic activity for monocyte-rich cells and PMN. These results, therefore, suggested that enhanced phagocytic activity of canine peripheral blood PMN and monocytes may be mainly mediated by humoral factor(S) released from PBMC treated with 1,2-benzopyrone.

키워드

참고문헌

  1. J Immunol v.149 Production of hemolymphopoietic cytokines (IL-6, IL-8, colony stimulating factors) by normal human astrocytes in response to Il-1β and tumor necrosis factor-α Aloisi,F.;Care,A.;Borsellino,G.;Gallo,P.;Rosa,S.;Bassani,A.;Cabibbo,A.;Testa,U.;Levi,G.;Peschle,C.
  2. N Engl J Med v.316 Cachetin: more than a tumor necrosis factor Beutler,B.;Carami,A.
  3. Ann Nutr Metab v.42 Protein malnutrition: some aspects of the in vitro adhesion of peritoneal mouse macrophages Borelli,P.;Souza,I.P.;Borojevic,R.;Dagli,M.L.;Kang,H.C.
  4. BMJ v.307 Treatment of filiarial lymphoedema and elephantiasis with 5,6 benzo-α-pyrone (coumarin) Casley-Smith,J.R.;Wang,C.T.;Zi-hai,C.
  5. Immunology v.69 Role of TNF and IL-1 in infections with Toxoplasma gondii Chang,H.r.;Grau,G.e.;Pechere,J.C.
  6. Vet J v.162 Evaluation in vitro of canine neutrophil function Comazzi,S.;Paltrinieri,S.;Spagnolo,V.;Sartorelli,P.
  7. Kuby immunology(4th ed.) Generation of B-cell and T-cell responses Goldsby,R.A.;Kindt,T.J.;Osborne,B.A.
  8. J Vet Clin v.20 Supplement of conjugated linoleic acid increases neutrophil phagocytosis in pigs Han,S.C.;Kang,J.H.;Son,S.M.;Chung,C.S.;Lee,C.Y.;Yang,M.P.
  9. Methods Find Exp Clin Phamacol v.5 The effect of coumarin and 7-hydroxycoumarin on in vitro macrophage phagocytosis of latex particles Hardt,T.J.;Ritschel,W.A.
  10. J Vet Med Sci v.57 Enhancing effects of chicken egg white derivatives on the phagocytic response in the dog Hirota,Y.;Yang,M.P.;Araki,S.;Yoshihara,K.;Furusawa,S.;Yasuda,M.;Mohamed,A.;Matsumoto,Y.;Onodera,T.
  11. Gen Pharmacol v.27 Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential Hoult,J.R.;Paya,M.
  12. J Vet Clin v.19 A case of malignant lymphedema in a dog Kang,J.H.;Lee,J.Y.;Han,S.C.;Son,S.M.;Na,K.J.;Mo,I.P.;Yang,M.P.
  13. Clin Sci v.77 Coumarin and 7-hydroxyocoumarin treatment of canine obstructive lymphoedema Knight,K.R.;Khazanchi,R.K.;Pederson,W.C.;McCann,J.J.;Coe,S.A.;O'Brien,B.M.
  14. thesis of master degree, Dept. Veterinary Medicine, Chungbuk National University tumor necrosis factor - α expression of porcine peripheral blood mononuclear cells induced by egg white derivatives Ko,I.K.
  15. Food Chem Toxicol v.37 Coumarin metabolism, toxicity and carcinogenicity: relevance for human risk assessment Lake,B.G.
  16. Exp Gerontol v.37 Effect of aging on macrophage function Lloberas,J.;Celada,A.
  17. J Vet Clin v.15 Effect of ginseng saponins on phagocytosis of feline peripheral blood phagocytes Park,S.H.;Yun,Y.w.;Yang,M.P.
  18. Lymphology v.13 Lymphoedema, macrophages and benzopyrones Piller,N.B.
  19. Methods Find Exp Clin Pharmacol v.13 Interspecies scaling of the pharmacokinetic parameters of coumarin among six different mammalian species Ritschel,W.A.;Vachharajani,N.N.;Johnson,R.D.;Hussain,A.S.
  20. Am J Med v.110 Lymphedema Rockson,S.G.
  21. Mech Ageing Dev v.124 neutrophil immunity of the elderly Schroder,A.K.;Rink,L.
  22. J Immunol Methods v.232 The use of flow cytometry to measure neutrophil function van Eeden SF;Klut,M.E.;Walker,B.A.;Hogg,J.c.
  23. J Vet Clin v.16 Detection of phagocytosis-promoting factor of culture supernatant from feline peripheral blood mononuclear cells cultured with egg white dervates Yang,M.P.;Kim,K.H.
  24. J Vet Clin v.16 Effect of egg white derivatives on phagocytic response of feline peripheral blood phagocytes Yang,M.P.;Kim,K.H.