Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
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Clinical Significance of p53 Gene and nm23 Gene Expression in Esophageal Cancer

식도암 조직에시 p53 및 nm23 유전자 발현의 임상적 의의

  • Park, Kuhn (Dept. of Thoracic and Cardivascular Surgery, Catholic University Medical College) ;
  • Lee, Jong-Ho ( Dept. of Thoracic and Cardivascular Surgery, Catholic University Medical College) ;
  • Sa, Young-Jo ( Dept. of Thoracic and Cardivascular Surgery, Catholic University Medical College) ;
  • Jin, Ung ( Dept. of Thoracic and Cardivascular Surgery, Catholic University Medical College) ;
  • Kwon, Jong-Bum ( Dept. of Thoracic and Cardivascular Surgery, Catholic University Medical College) ;
  • Park, Jae-Gil ( Dept. of Thoracic and Cardivascular Surgery, Catholic University Medical College) ;
  • Lee, Sun-He ( Dept. of Thoracic and Cardivascular Surgery, Catholic University Medical College) ;
  • Kwak, Moon-Sub ( Dept. of Thoracic and Cardivascular Surgery, Catholic University Medical College)
  • 박건 (가톨릭대학교 의과대학 흉부외과학교실) ;
  • 이종호 (가톨릭대학교 의과대학 흉부외과학교실) ;
  • 사영조 (가톨릭대학교 의과대학 흉부외과학교실) ;
  • 진웅 (가톨릭대학교 의과대학 흉부외과학교실) ;
  • 권종범 (가톨릭대학교 의과대학 흉부외과학교실) ;
  • 박재길 (가톨릭대학교 의과대학 흉부외과학교실) ;
  • 이선희 (가톨릭대학교 의과대학 흉부외과학교실) ;
  • 곽문섭 (가톨릭대학교 의과대학 흉부외과학교실)
  • Published : 2004.03.01
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Abstract

Although significant progress has been made in the surgical treatment of esophageal carcinoma as well as in the detection of early stage esophageal carcinoma by diagnostic techniques, the prognosis of the esophageal carcinoma patients remain poor. The p53 gene product is known to regulate cell growth and proliferation. And the nm23 gene was identified originally as an anti-metastatic influence whose expression was correlated inversely with tumor metastatic potential in murine melanoma cell lines. This experiment was intended to know the relationship among the p53 and nm23 gene expression versus clinicopahologic characteristics of the esophageal cancer. Total 40 cases were collected from patients who had undergone esophagectomy at St. Mary's Hospital, Catholic university of Korea. Immunohistochemical stain for p53 mutant-type protein and nm23 protein was graded as <10% positive tumor cells: negative; 10∼30% positive tumor cells: ＋ ; 30∼50% positive tumor cells: ＋＋, and >50% positive tumor cells: ＋＋＋. The tumor invasion was grades as none:－ ; mild:＋ ; moderate:＋＋ ; severe: ＋＋＋. Overexpression of p53 protein and nm23 was not associated with the survival and cliniocopathologic characteristics of the esophageal cancer. Moreover, the combination analysis of p53 and nm23 revealed that there was no relationship between the gene expression and the clinicopatholic characteristics of the esophageal cancer.

식도암 환자에서 조기진단 및 수술적 치료 방법의 상당한 진전에도 불구하고 환자의 예후는 여전히 좋지 않다. p53 종양 억제유전자는 세포의 성장과 증식을 조절하는 것으로 알려져 있고 nm23 유전자는 설치류 흑색종에서 종양의 전이억제 효과가 있다고 알려져 있다. 이 실험은 p53과 nm23유전자 발현과 식도암 환자의 임상병리학적인 특징상의 관련성을 알아보고자 하였다. 가톨릭대학교 의과대학 부속 성모병원에서 수술한 식도암 환자 40명의 조직을 대상으로 하였고, p53 변이형 단백질과 nm23단백질을 면역화학적 염색을 시행하여 <10% 양성 종양세포 : negative ; 10∼30% 양성 종양세포: ＋; 30∼50% 양성 종양세포 : ＋＋; >50% 양성 종양세포: ＋＋＋의 4개의 군으로 분류하였고, 또한 종양의 침습 정도는 none, mild, moderate, severe로 분류하여 평가하였다. p53 변이형 단백질과 nm23 단백질의 과발현은 생존율 및 임상병리학적 특징과 연관성이 없었고, 또한 p53 및 nm23유전자 발현의 조합 분석에서도 유의한 상관관계를 발견하지 못하였다.

Keywords

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