Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant Regeneration from Adventitious Roots of Rehmannia glutinosa Liboschitz and Bioreactor Culture

지황 부정근을 이용한 식물체 재분화 및 생물반응기 배양

  • Jeong, Jae-Hun (Division of Forest Genetics Resources, Korea Forest Research Institute) ;
  • Yu, Kee-Won (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Kim, Sun-Ja (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Choi, Yong-Eui (Division of Forest Resources, College of Forest Sciences, Kangwon National University) ;
  • Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
  • 정재훈 (국립산림과학원 생물공학과) ;
  • 유기원 (충북대학교 첨단원예기술개발연구센터) ;
  • 김선자 (충북대학교 첨단원예기술개발연구센터) ;
  • 최용의 (강원대학교 산림과학대학 산림자원학부) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • Published : 2004.03.01
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Abstract

This experiment was carried out to develop rapid mass propagation via shoot organogenesis system from adventitious roots of Rehmannia glutinosa. The induction of adventitious roots from leaf explants was most favorable to MS solid medium supplemented with 2mg/L IBA. However, the growth of adventitious roots was highest when they were cultured on 1/3 strength MS liquid medium supplemented with 2mg/L IBA. When the adventitious roots were grown in 10L bioreactor, 10g roots as initial inoculum was increased to 225g after 6 weeks of culture. The harvested roots were cultured onto solid medium to induce plant regeneration. The optimal adventitious shoot formation was observed on MS medium supplemented with 2mg/L BA. Rooting of individual shoots was induced after transfer to half strength MS medium without growth regulators. Plantlets after acclimatization were successfully transplanted in the field and no phenotypic variation was observed among them.

지황의 부정근을 이용한 기내묘 생산 체계를 확립하였다. 먼저 지황의 잎절편을 2 mg/L IBA혹은 2mg/L NAA를 첨가한 MS고체배지에 치상하여 부정근을 유도하였다. 고체배지에서의 효율적인 증식조건을 확인하기 위해 다양한 옥신 농도에 따른 배지에 부정근을 치상하여 4주간 배양한 후 증식된 부정근의 수와 길이를 측정한 결과 2mg/L IBA조건에서 최적의 생장을 보였다. 상기실험에서 확립된 부정근 증식조건을 이용하여 액체배양을 실시하였으며, 이를 액체 현탁배양하여 액체조건에서의 증식조건을 확립하였다. 또한, 2mg/L IBA가 첨가된 1/3 MS배지 조건에서 생물반응기에 10g의 부정근을 접종하여 6주간 암배양하여 255g의 부정근을 생산할 수 있었으며, 이렇게 생산된 부정근은 2mg/L BA가 첨가된 MS 고체배지에서 신초를 유도하였다. 신초들은 모두 정상적인 생육을 보이며, 식물체로 생장하였으며, 이들 식물체들은 토양으로 옮겨 순화되었다. 따라서 이러한 증식 체계를 이용하여 지황식물체의 대량증식체계를 확립하였다.

Keywords

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