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In Vitro Propagation of Alocasia cadieri Chantrier

Alocasia cadieri Chantrier의 기내번식

  • Han, Bong-Hee (Horticulture Biotechnology Division, Horticulture Research Institute) ;
  • Yae, Byeoung-Woo (Research Coordination Division, Rural development Administration) ;
  • Goo, Dae-Hoe (Floriculture Research Division, Horticulture Research Institute) ;
  • Yu, Hee-Ju (Horticulture Biotechnology Division, Horticulture Research Institute)
  • 한봉희 (원예 연구소 원예생명공학과) ;
  • 예병우 (농촌진흥청 연구관리과) ;
  • 구대회 (원예연구소 화훼과) ;
  • 유희주 (원예 연구소 원예생명공학과)
  • Published : 2004.03.01

Abstract

In order to micropropagate uniform plantlets of Alocasia cadieri Chantrier in vitro, the shoot tips were cultured on media containing various concentrations of BA and thidiazuron (TDZ). Multiple shoot formation from shoot tips was very effective on medium containing 0.1mg/L TDZ. The formed shoots from shoot tips were separated into a shoot, and cultured on media with BA, TDZ, and NM combination for proliferation. The shoots were multiplied very vigorously on medium with 0.5mg/L TDZ and 0.5mg/L NAA. The rooting and growth of multiplied shoots were more effective on medium with 2.0g/L activated charcoal, rather than those with IBA and NAA. Rooted plantlets show high survival in soil mixed with perlite 1: vermiculite 1 or vermiculite alone.

본 실험은 Alocasia cadieri Chantrier를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Alocasia의 경정을 TDZ 0.1mg/L가 첨가된 배지에 배양하였을 때 신초증식이 가장 높았으며, 형성된 신초에서 신초의 증식은 TDZ과 NAA가 각각 0.5mg/L첨가된 배지에서 효과적이었다. 형성된 신초의 발근은 IBA또는 NAA가 첨가된 배지보다는 활성탄 2.0g/L가 첨가된 배지에서 양호하였으며, 소식물체의 순화는 perlite : vermiculite가 1:1로 혼합된 용토 또는 vermiculite가 적합하였다.

Keywords

References

  1. Earle ED, Langhans RW (1974) Propagation of Chrysanthemum in vitro. I. Multiple plantlets from shoot tips and the establishment of tissue culture. J Amer Soc Hort Sci 99:128-132
  2. Ebert A, Taylor HF (1990) Assessment of changes of 2,4-dichlorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal. Plant Cell Tiss Org Cult 32:223-225
  3. Fellman CD, Read PE, Hosier MA (1987) Effect of thidiazuron and CPPU on meristem formation and shoot proliferation. HortScience 22:1197-1200
  4. Fridborg G, Pedersen M, Landstrom LE, Eriksson T (1978) The effects of activated charcoal on tissue culture: absorption of metabolites inhibiting motphogenesis. Physiol Plant 43: 104-106 https://doi.org/10.1111/j.1399-3054.1978.tb01575.x
  5. Hackett WP (1985) Juvenility, maturation and rejuvenation in wood plants. Hort Rev 7:109-158
  6. Han BH, Joung HY, Ko JY (1997) In vitro propagation of Ficus benjamina by shoot tip culture. J Kor Soc Hort Sci 38:315-319
  7. Kusey WE, Hammer PA, Weiler TC (1980) In vitro propagation of Gypsophila paniculata L. 'Bristol Fairy'. Hort Science 15:600-601
  8. Maesato K, Sharada K, Fukui H, Hara T, Sarma KS (1994) in vitro bulblet regulation from bulbscale explants of Litlium japonicum Thunb. Effect of plant growth regulators and culture environment. J Hort Sci 69: 289-297
  9. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  10. Novak FJ, Petru E (1981) Tissue culture propagation of Lilium hybrids. Sci Hort 14:191-199 https://doi.org/10.1016/0304-4238(81)90012-1
  11. Pennazio S (1975) Effect of adenine and kinetin on development of camation tips cultured in vitro. J Hort Sci 50:161-164
  12. Reynolds JF (1987) Chemical regulation in tissue culture: An overview. Hort Science 22:1192-1194
  13. Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation. Sym Soc Exp Biol 11:118-131
  14. Takayama S, Misawa M (1982) Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in uitro. Plant Cell Physiol 23: 67-74
  15. Thao NTP, Ozaki Y, Okubo H (2003a) Callus induction and plantlet regeneration in ornamental Alocasia micholitziana. PIant Cell Tiss Org CuIt 73: 285-289 https://doi.org/10.1023/A:1023025717271
  16. Thao NTP, Ureshino K, Miyajima I, Ozaki Y, Okubo H (2003b) Introduction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell Tiss Org Cult 72:19 25 https://doi.org/10.1023/A:1021292928295
  17. Weatherhead MA, Burdon J, Henshaw GG (1978) Some effects of activated charcoal as an additive to plant tissue culture media. Z Pflanzenphysiol 89:141-147 https://doi.org/10.1016/S0044-328X(78)80054-3