Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Rapid Quantitative Analysis of Isoflavones using TLC

TLC를 이용한 이소플라본의 신속한 정량 분석

  • Kim, Kyung-Seon (Department of Food Science and Biotechnology, KyungHee University) ;
  • Park, Kwan-Hwa (Department of Food Science and Technology, Seoul National University) ;
  • Baik, Moo-Yeol (Department of Food Science and Biotechnology, KyungHee University) ;
  • Kang, Kil-Jin (Containers and Packaging Division, Korea Food and Drug Administration) ;
  • Park, Cheon-Seok (Department of Food Science and Biotechnology, KyungHee University)
  • 김경선 (경희대학교 생명과학대학 식품공학전공) ;
  • 박관화 (서울대학교 농생명과학대학 식품공학과) ;
  • 백무열 (경희대학교 생명과학대학 식품공학전공) ;
  • 강길진 (식품의약품안전청 용기포장과) ;
  • 박천석 (경희대학교 생명과학대학 식품공학전공)
  • Published : 2004.08.31
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Abstract

Conditions for rapid quantification of isoflavones were studied. Rapid and clear separation of isoflavones (genistin and daidzin) was obtained using solvent system of chloroform : methanol : water : acetic acid (60 : 30 : 10 : 0.5, v/v/v/v). Quantification of each isoflavone separated by TLC was conducted by densitometry analysis. Genistin and daidzin were quantified in $0.15-1.80\;{\mu}g/{\mu}L$ range with 99% confidence. Concentrations of isoflavones in soybeans and kudzu roots originated from Korea were determined, and validity of TLC method for quantification of isoflavones was confirmed by comparison with HPLC analysis.

TLC를 이용하여 간편하고 신속하게 정량적인 이소플라본의 분석을 수행할 수 있는 조건을 개발하였다. 전개 용매로는 chloroform : methanol : water : acetic acid를 각각 60 : 30 : 10 : 0.5의 비율로 하였을 때 최적의 분리를 보였으며, 정량적인 분석은 UV에서의 흡광도를 densitometer를 사용하여 spot의 intensity를 계산함으로 가능하였다. HPLC분석법과의 비교 결과 $0.15-1.80{\mu}g/{\mu}L$의 범위에서 이소플라본을 정량적으로 분석할 수가 있었다. 또한 TLC를 이용하여 일반 콩과 식물에 포함되어 있는 이소플라본의 함량 측정 결과 HPLC의 결과와 상당히 근소한 값으로 일치하여 TLC방법으로 간편하고 빠르게 정량적인 측정이 가능함을 확인하였다.

Keywords

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